Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System
The Atlantic cod’s unusual immune system, entirely lacking the Major Histocompatibility class II pathway, has prompted intriguing questions about what mechanisms are used to combat bacterial infections and how immunological memory is generated. By single-cell RNA sequencing we here report an in-dept...
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ftdoajarticles:oai:doaj.org/article:3bfaf14431304939a4a3652f9b77b580 2023-05-15T15:26:42+02:00 Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao 2020-10-01T00:00:00Z https://doi.org/10.3389/fimmu.2020.559555 https://doaj.org/article/3bfaf14431304939a4a3652f9b77b580 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fimmu.2020.559555/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.559555 https://doaj.org/article/3bfaf14431304939a4a3652f9b77b580 Frontiers in Immunology, Vol 11 (2020) Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker Immunologic diseases. Allergy RC581-607 article 2020 ftdoajarticles https://doi.org/10.3389/fimmu.2020.559555 2022-12-30T22:57:42Z The Atlantic cod’s unusual immune system, entirely lacking the Major Histocompatibility class II pathway, has prompted intriguing questions about what mechanisms are used to combat bacterial infections and how immunological memory is generated. By single-cell RNA sequencing we here report an in-depth characterisation of cell types found in immune tissues, the spleen and peripheral blood leukocytes of Atlantic cod. Unbiased transcriptional clustering revealed eleven distinct immune cell signatures. Resolution at the single cell level enabled characterisation of the major cell subsets including the cytotoxic T cells, B cells, erythrocytes, thrombocytes, neutrophils, and macrophages. Additionally, to our knowledge we are the first to uncover cell subsets in Atlantic cod which may represent dendritic cells, natural killer-like cells, and a population of cytotoxic cells expressing GATA-3, a master transcription factor of T helper 2 cells. We further identify putative gene markers for each cluster and describe the relative proportions of each cell type in the spleen and peripheral blood leukocytes. Of the major haematopoietic cell populations, the lymphocytes make up 55 and 68% of the spleen and peripheral blood leukocytes respectively, while the myeloid cells make up 45 and 32%. By single-cell analysis, this study provides the most detailed molecular and cellular characterisation of the immune system of the Atlantic cod so far. Article in Journal/Newspaper atlantic cod Gadus morhua Directory of Open Access Journals: DOAJ Articles Gata ENVELOPE(-19.702,-19.702,63.540,63.540) Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker Immunologic diseases. Allergy RC581-607 Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System |
topic_facet |
Atlantic cod (Gadus morhua) single-cell sequencing GATA-3 immune system gene marker Immunologic diseases. Allergy RC581-607 |
description |
The Atlantic cod’s unusual immune system, entirely lacking the Major Histocompatibility class II pathway, has prompted intriguing questions about what mechanisms are used to combat bacterial infections and how immunological memory is generated. By single-cell RNA sequencing we here report an in-depth characterisation of cell types found in immune tissues, the spleen and peripheral blood leukocytes of Atlantic cod. Unbiased transcriptional clustering revealed eleven distinct immune cell signatures. Resolution at the single cell level enabled characterisation of the major cell subsets including the cytotoxic T cells, B cells, erythrocytes, thrombocytes, neutrophils, and macrophages. Additionally, to our knowledge we are the first to uncover cell subsets in Atlantic cod which may represent dendritic cells, natural killer-like cells, and a population of cytotoxic cells expressing GATA-3, a master transcription factor of T helper 2 cells. We further identify putative gene markers for each cluster and describe the relative proportions of each cell type in the spleen and peripheral blood leukocytes. Of the major haematopoietic cell populations, the lymphocytes make up 55 and 68% of the spleen and peripheral blood leukocytes respectively, while the myeloid cells make up 45 and 32%. By single-cell analysis, this study provides the most detailed molecular and cellular characterisation of the immune system of the Atlantic cod so far. |
format |
Article in Journal/Newspaper |
author |
Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao |
author_facet |
Naomi Croft Guslund Monica Hongrø Solbakken Marine S. O. Brieuc Sissel Jentoft Kjetill S. Jakobsen Shuo-Wang Qiao |
author_sort |
Naomi Croft Guslund |
title |
Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System |
title_short |
Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System |
title_full |
Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System |
title_fullStr |
Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System |
title_full_unstemmed |
Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System |
title_sort |
single-cell transcriptome profiling of immune cell repertoire of the atlantic cod which naturally lacks the major histocompatibility class ii system |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fimmu.2020.559555 https://doaj.org/article/3bfaf14431304939a4a3652f9b77b580 |
long_lat |
ENVELOPE(-19.702,-19.702,63.540,63.540) |
geographic |
Gata |
geographic_facet |
Gata |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Frontiers in Immunology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fimmu.2020.559555/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2020.559555 https://doaj.org/article/3bfaf14431304939a4a3652f9b77b580 |
op_doi |
https://doi.org/10.3389/fimmu.2020.559555 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1766357183608389632 |