A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos
Between 1992 and 1997, 790 blood donors with anti-HCV EIA-2 strongly reagent (relationship between the sample optical density/cut-off > 3) detected at the blood bank serological screening, were evaluated in ambulatory environment. They were all negative for Chagas disease, syphilis, hepatitis B (...
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ftdoajarticles:oai:doaj.org/article:3bef711177cb40048b39fe69e88aa879 2024-09-09T19:28:39+00:00 A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos Fernando L. GONÇALES JR Raquel S. B. STUCCHI Maria Helena P. PAVAN Cecília A. F. ESCANHOELA Ademar YAMANAKA Luís Alberto MAGNA Neiva S. L. GONÇALES 2000-06-01T00:00:00Z https://doi.org/10.1590/S0036-46652000000300007 https://doaj.org/article/3bef711177cb40048b39fe69e88aa879 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000300007 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652000000300007 0036-4665 1678-9946 https://doaj.org/article/3bef711177cb40048b39fe69e88aa879 Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 3, Pp 147-152 (2000) Anti-HCV Blood donors Viral Hepatitis Hepatitis C Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2000 ftdoajarticles https://doi.org/10.1590/S0036-46652000000300007 2024-08-05T17:49:30Z Between 1992 and 1997, 790 blood donors with anti-HCV EIA-2 strongly reagent (relationship between the sample optical density/cut-off > 3) detected at the blood bank serological screening, were evaluated in ambulatory environment. They were all negative for Chagas disease, syphilis, hepatitis B (HBsAg) and AIDS. Blood samples were collected at the first ambulatorial evaluation, for hemogram, biochemical tests and new serological tests for HCV (anti-HCV EIA-2). In blood samples of 226 repeatedly reagent anti-HCV EIA-2 blood donors, supplementary "immunoblot" test for HCV (RIBA-2) was used. In 209 donors, the presence of HCV-RNA was investigated by the PCR test. The abdominal ultrasonography was realized in 366 donors. In 269 patients liver biopsy was performed for the histopathological study. The follow-up of blood donors showed that 95.6% were repeatedly EIA-2 reagent, 94% were symptomless and denied any hepatitis history, with only 2% mentioning previous jaundice. In 47% of this population at least one risk factor has been detected for the HCV transmission, the use of intravenous drugs being the main one (27.8%). Blood transfusion was the second factor for HCV transmission (27.2%). Hepatomegaly was detected in 54% of the cases. Splenomegaly and signs of portal hypertension have seldom been found in the physical examination, indicating a low degree of hepatic compromising in HCV. Abdominal ultrasound showed alterations in 65% of the subjects, being the steatosis the most frequent (50%). In 83.5% of the donors submitted to the liver biopsy, the histopathological exam showed the presence of chronic hepatitis, usually classified as active (89%) with mild or moderate grade in most of the cases (99.5%). The histopathological exam of the liver was normal in 1.5% of blood donors. The RIBA-2 test and the HCV-RNA investigation by PCR were positive in respectively 91.6 and 75% of the anti-HCV EIA-2 reagent donors. The HCV-RNA research was positive in 82% of the RIBA-2 positive subjects, in 37.5% of the indeterminate ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eia ENVELOPE(7.755,7.755,63.024,63.024) Revista do Instituto de Medicina Tropical de São Paulo 42 3 147 152 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anti-HCV Blood donors Viral Hepatitis Hepatitis C Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Anti-HCV Blood donors Viral Hepatitis Hepatitis C Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Fernando L. GONÇALES JR Raquel S. B. STUCCHI Maria Helena P. PAVAN Cecília A. F. ESCANHOELA Ademar YAMANAKA Luís Alberto MAGNA Neiva S. L. GONÇALES A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos |
topic_facet |
Anti-HCV Blood donors Viral Hepatitis Hepatitis C Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Between 1992 and 1997, 790 blood donors with anti-HCV EIA-2 strongly reagent (relationship between the sample optical density/cut-off > 3) detected at the blood bank serological screening, were evaluated in ambulatory environment. They were all negative for Chagas disease, syphilis, hepatitis B (HBsAg) and AIDS. Blood samples were collected at the first ambulatorial evaluation, for hemogram, biochemical tests and new serological tests for HCV (anti-HCV EIA-2). In blood samples of 226 repeatedly reagent anti-HCV EIA-2 blood donors, supplementary "immunoblot" test for HCV (RIBA-2) was used. In 209 donors, the presence of HCV-RNA was investigated by the PCR test. The abdominal ultrasonography was realized in 366 donors. In 269 patients liver biopsy was performed for the histopathological study. The follow-up of blood donors showed that 95.6% were repeatedly EIA-2 reagent, 94% were symptomless and denied any hepatitis history, with only 2% mentioning previous jaundice. In 47% of this population at least one risk factor has been detected for the HCV transmission, the use of intravenous drugs being the main one (27.8%). Blood transfusion was the second factor for HCV transmission (27.2%). Hepatomegaly was detected in 54% of the cases. Splenomegaly and signs of portal hypertension have seldom been found in the physical examination, indicating a low degree of hepatic compromising in HCV. Abdominal ultrasound showed alterations in 65% of the subjects, being the steatosis the most frequent (50%). In 83.5% of the donors submitted to the liver biopsy, the histopathological exam showed the presence of chronic hepatitis, usually classified as active (89%) with mild or moderate grade in most of the cases (99.5%). The histopathological exam of the liver was normal in 1.5% of blood donors. The RIBA-2 test and the HCV-RNA investigation by PCR were positive in respectively 91.6 and 75% of the anti-HCV EIA-2 reagent donors. The HCV-RNA research was positive in 82% of the RIBA-2 positive subjects, in 37.5% of the indeterminate ... |
format |
Article in Journal/Newspaper |
author |
Fernando L. GONÇALES JR Raquel S. B. STUCCHI Maria Helena P. PAVAN Cecília A. F. ESCANHOELA Ademar YAMANAKA Luís Alberto MAGNA Neiva S. L. GONÇALES |
author_facet |
Fernando L. GONÇALES JR Raquel S. B. STUCCHI Maria Helena P. PAVAN Cecília A. F. ESCANHOELA Ademar YAMANAKA Luís Alberto MAGNA Neiva S. L. GONÇALES |
author_sort |
Fernando L. GONÇALES JR |
title |
A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos |
title_short |
A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos |
title_full |
A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos |
title_fullStr |
A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos |
title_full_unstemmed |
A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos |
title_sort |
clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-hcv eia-2 positive blood donors avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-hcv eia-2 positivos |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2000 |
url |
https://doi.org/10.1590/S0036-46652000000300007 https://doaj.org/article/3bef711177cb40048b39fe69e88aa879 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Arctic Eia |
geographic_facet |
Arctic Eia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 3, Pp 147-152 (2000) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000300007 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652000000300007 0036-4665 1678-9946 https://doaj.org/article/3bef711177cb40048b39fe69e88aa879 |
op_doi |
https://doi.org/10.1590/S0036-46652000000300007 |
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