sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis.
BACKGROUND:CD163, receptor for the haptoglobin-hemoglobin complex, is expressed on monocytes/macrophages and neutrophils. A soluble form of CD163 (sCD163) has been associated with the M2 macrophage phenotype, and M2 macrophages have been shown to down-modulate inflammatory responses. In particular,...
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ftdoajarticles:oai:doaj.org/article:3bd58a6b43854163afaeb1537a98a6a7 2023-05-15T15:11:17+02:00 sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. Ricardo Luís Louzada Silva Marcio B Santos Priscila L S Almeida Thayse S Barros Lucas Magalhães Rodrigo A Cazzaniga Patrícia R M Souza Nívea F Luz Jaqueline França-Costa Valeria M Borges Djalma S Lima-Junior Michael W Lipscomb Malcolm S Duthie Steven G Reed Roque Pacheco Almeida Amélia Ribeiro Jesus 2017-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005486 https://doaj.org/article/3bd58a6b43854163afaeb1537a98a6a7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5386291?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005486 https://doaj.org/article/3bd58a6b43854163afaeb1537a98a6a7 PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005486 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005486 2022-12-31T11:01:37Z BACKGROUND:CD163, receptor for the haptoglobin-hemoglobin complex, is expressed on monocytes/macrophages and neutrophils. A soluble form of CD163 (sCD163) has been associated with the M2 macrophage phenotype, and M2 macrophages have been shown to down-modulate inflammatory responses. In particular, previous studies have shown that M2 is closely associated with the most severe clinical presentation of leprosy (i.e. lepromatous leprosy (LL)), as well as tuberculosis. We hypothesized that sCD163 correlates with severity of diseases caused by intracellular pathogens. METHODOLOGY/PRINCIPAL FINDINGS:To assess this hypothesis, sCD163 levels were measured in the serum of leprosy and visceral leishmaniasis (VL) patients stratified by severity of the clinical presentation. sCD163 levels were significantly higher in patients with these diseases than those observed in healthy control individuals. Further analyses on infection and disease status of leprosy and VL patients revealed a clear association of sCD163 levels with clinical parameters of disease severity. In vitro culture assays revealed that Leishmania infection induced CD163 expression on the surface of both monocyte/macrophages and neutrophils, suggesting these cells as possible sources of sCD163. FACS analyses shows that the cells expressing CD163 produces both TNF-α and IL-4. CONCLUSIONS/SIGNIFICANCE:Taken together, our results reveal sCD163 as a potential biomarker of severity of diseases caused by intracellular pathogens M. leprae and Leishmania spp. and have a modulatory role, with a mix of an inflammatory property induced by TNF-α release, but that potentially induces an anti-inflammatory T cell response, related to IL-4 release. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 3 e0005486 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ricardo Luís Louzada Silva Marcio B Santos Priscila L S Almeida Thayse S Barros Lucas Magalhães Rodrigo A Cazzaniga Patrícia R M Souza Nívea F Luz Jaqueline França-Costa Valeria M Borges Djalma S Lima-Junior Michael W Lipscomb Malcolm S Duthie Steven G Reed Roque Pacheco Almeida Amélia Ribeiro Jesus sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:CD163, receptor for the haptoglobin-hemoglobin complex, is expressed on monocytes/macrophages and neutrophils. A soluble form of CD163 (sCD163) has been associated with the M2 macrophage phenotype, and M2 macrophages have been shown to down-modulate inflammatory responses. In particular, previous studies have shown that M2 is closely associated with the most severe clinical presentation of leprosy (i.e. lepromatous leprosy (LL)), as well as tuberculosis. We hypothesized that sCD163 correlates with severity of diseases caused by intracellular pathogens. METHODOLOGY/PRINCIPAL FINDINGS:To assess this hypothesis, sCD163 levels were measured in the serum of leprosy and visceral leishmaniasis (VL) patients stratified by severity of the clinical presentation. sCD163 levels were significantly higher in patients with these diseases than those observed in healthy control individuals. Further analyses on infection and disease status of leprosy and VL patients revealed a clear association of sCD163 levels with clinical parameters of disease severity. In vitro culture assays revealed that Leishmania infection induced CD163 expression on the surface of both monocyte/macrophages and neutrophils, suggesting these cells as possible sources of sCD163. FACS analyses shows that the cells expressing CD163 produces both TNF-α and IL-4. CONCLUSIONS/SIGNIFICANCE:Taken together, our results reveal sCD163 as a potential biomarker of severity of diseases caused by intracellular pathogens M. leprae and Leishmania spp. and have a modulatory role, with a mix of an inflammatory property induced by TNF-α release, but that potentially induces an anti-inflammatory T cell response, related to IL-4 release. |
format |
Article in Journal/Newspaper |
author |
Ricardo Luís Louzada Silva Marcio B Santos Priscila L S Almeida Thayse S Barros Lucas Magalhães Rodrigo A Cazzaniga Patrícia R M Souza Nívea F Luz Jaqueline França-Costa Valeria M Borges Djalma S Lima-Junior Michael W Lipscomb Malcolm S Duthie Steven G Reed Roque Pacheco Almeida Amélia Ribeiro Jesus |
author_facet |
Ricardo Luís Louzada Silva Marcio B Santos Priscila L S Almeida Thayse S Barros Lucas Magalhães Rodrigo A Cazzaniga Patrícia R M Souza Nívea F Luz Jaqueline França-Costa Valeria M Borges Djalma S Lima-Junior Michael W Lipscomb Malcolm S Duthie Steven G Reed Roque Pacheco Almeida Amélia Ribeiro Jesus |
author_sort |
Ricardo Luís Louzada Silva |
title |
sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
title_short |
sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
title_full |
sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
title_fullStr |
sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
title_full_unstemmed |
sCD163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
title_sort |
scd163 levels as a biomarker of disease severity in leprosy and visceral leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005486 https://doaj.org/article/3bd58a6b43854163afaeb1537a98a6a7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005486 (2017) |
op_relation |
http://europepmc.org/articles/PMC5386291?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005486 https://doaj.org/article/3bd58a6b43854163afaeb1537a98a6a7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005486 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
3 |
container_start_page |
e0005486 |
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1766342164899430400 |