Isolation and characterization of Arctic microorganisms decomposing bioplastics
Abstract The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Amo...
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ftdoajarticles:oai:doaj.org/article:3bc5b0ce55e24e97acf00ec05f2d6e09 2023-05-15T14:47:06+02:00 Isolation and characterization of Arctic microorganisms decomposing bioplastics Aneta K. Urbanek Waldemar Rymowicz Mateusz C. Strzelecki Waldemar Kociuba Łukasz Franczak Aleksandra M. Mirończuk 2017-07-01T00:00:00Z https://doi.org/10.1186/s13568-017-0448-4 https://doaj.org/article/3bc5b0ce55e24e97acf00ec05f2d6e09 EN eng SpringerOpen http://link.springer.com/article/10.1186/s13568-017-0448-4 https://doaj.org/toc/2191-0855 doi:10.1186/s13568-017-0448-4 2191-0855 https://doaj.org/article/3bc5b0ce55e24e97acf00ec05f2d6e09 AMB Express, Vol 7, Iss 1, Pp 1-10 (2017) Biodegradation Biodegradable plastics (BP) Arctic microorganisms Microbial degradation Biotechnology TP248.13-248.65 Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.1186/s13568-017-0448-4 2022-12-31T14:29:36Z Abstract The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Among the isolated microorganisms, 121 (38.66%) showed biodegradation activity. The ability of clear zone formation on emulsified poly(butylene succinate-co-adipate) (PBSA) was observed for 116 microorganisms (95.87%), on poly(butylene succinate) (PBS) for 73 microorganisms (60.33%), and on poly(ɛ-caprolactone) (PCL) for 102 microorganisms (84.3%). Moreover, the growth of microorganisms on poly(lactic acid) (PLA) agar plates was observed for 56 microorganisms (46.28%). Based on the 16S rRNA sequence, 10 bacterial strains which showed the highest ability for biodegradation were identified as species belonging to Pseudomonas sp. and Rhodococcus sp. The isolated fungal strains were tested for polycaprolactone films and commercial corn and potato starch bags degradation under laboratory conditions. Strains 16G (based on the analysis of a partial 18S rRNA sequence, identified as Clonostachys rosea) and 16H (identified as Trichoderma sp.) showed the highest capability for biodegradation. A particularly high capability for biodegradation was observed for the strain Clonostachys rosea, which showed 100% degradation of starch films and 52.91% degradation of PCL films in a 30-day shake flask experiment. The main advantage of the microorganisms isolated from Arctic environment is the ability to grow at low temperature and efficient biodegradation under this condition. The data suggest that C. rosea can be used in natural and laboratory conditions for degradations of bioplastics. Article in Journal/Newspaper Arctic Spitsbergen Directory of Open Access Journals: DOAJ Articles Arctic AMB Express 7 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Biodegradation Biodegradable plastics (BP) Arctic microorganisms Microbial degradation Biotechnology TP248.13-248.65 Microbiology QR1-502 |
spellingShingle |
Biodegradation Biodegradable plastics (BP) Arctic microorganisms Microbial degradation Biotechnology TP248.13-248.65 Microbiology QR1-502 Aneta K. Urbanek Waldemar Rymowicz Mateusz C. Strzelecki Waldemar Kociuba Łukasz Franczak Aleksandra M. Mirończuk Isolation and characterization of Arctic microorganisms decomposing bioplastics |
topic_facet |
Biodegradation Biodegradable plastics (BP) Arctic microorganisms Microbial degradation Biotechnology TP248.13-248.65 Microbiology QR1-502 |
description |
Abstract The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Among the isolated microorganisms, 121 (38.66%) showed biodegradation activity. The ability of clear zone formation on emulsified poly(butylene succinate-co-adipate) (PBSA) was observed for 116 microorganisms (95.87%), on poly(butylene succinate) (PBS) for 73 microorganisms (60.33%), and on poly(ɛ-caprolactone) (PCL) for 102 microorganisms (84.3%). Moreover, the growth of microorganisms on poly(lactic acid) (PLA) agar plates was observed for 56 microorganisms (46.28%). Based on the 16S rRNA sequence, 10 bacterial strains which showed the highest ability for biodegradation were identified as species belonging to Pseudomonas sp. and Rhodococcus sp. The isolated fungal strains were tested for polycaprolactone films and commercial corn and potato starch bags degradation under laboratory conditions. Strains 16G (based on the analysis of a partial 18S rRNA sequence, identified as Clonostachys rosea) and 16H (identified as Trichoderma sp.) showed the highest capability for biodegradation. A particularly high capability for biodegradation was observed for the strain Clonostachys rosea, which showed 100% degradation of starch films and 52.91% degradation of PCL films in a 30-day shake flask experiment. The main advantage of the microorganisms isolated from Arctic environment is the ability to grow at low temperature and efficient biodegradation under this condition. The data suggest that C. rosea can be used in natural and laboratory conditions for degradations of bioplastics. |
format |
Article in Journal/Newspaper |
author |
Aneta K. Urbanek Waldemar Rymowicz Mateusz C. Strzelecki Waldemar Kociuba Łukasz Franczak Aleksandra M. Mirończuk |
author_facet |
Aneta K. Urbanek Waldemar Rymowicz Mateusz C. Strzelecki Waldemar Kociuba Łukasz Franczak Aleksandra M. Mirończuk |
author_sort |
Aneta K. Urbanek |
title |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_short |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_full |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_fullStr |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_full_unstemmed |
Isolation and characterization of Arctic microorganisms decomposing bioplastics |
title_sort |
isolation and characterization of arctic microorganisms decomposing bioplastics |
publisher |
SpringerOpen |
publishDate |
2017 |
url |
https://doi.org/10.1186/s13568-017-0448-4 https://doaj.org/article/3bc5b0ce55e24e97acf00ec05f2d6e09 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
AMB Express, Vol 7, Iss 1, Pp 1-10 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s13568-017-0448-4 https://doaj.org/toc/2191-0855 doi:10.1186/s13568-017-0448-4 2191-0855 https://doaj.org/article/3bc5b0ce55e24e97acf00ec05f2d6e09 |
op_doi |
https://doi.org/10.1186/s13568-017-0448-4 |
container_title |
AMB Express |
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7 |
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1 |
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1766318218956242944 |