A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species
This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For th...
Published in: | Potravinarstvo Slovak Journal of Food Sciences |
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Language: | English |
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HACCP Consulting
2022
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Online Access: | https://doi.org/10.5219/1777 https://doaj.org/article/3bb542c0b1824287af8eab45f71b251b |
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ftdoajarticles:oai:doaj.org/article:3bb542c0b1824287af8eab45f71b251b 2023-05-15T16:19:24+02:00 A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno 2022-10-01T00:00:00Z https://doi.org/10.5219/1777 https://doaj.org/article/3bb542c0b1824287af8eab45f71b251b EN eng HACCP Consulting https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 https://doaj.org/toc/1337-0960 doi:10.5219/1777 1337-0960 https://doaj.org/article/3bb542c0b1824287af8eab45f71b251b Potravinarstvo, Vol 16 (2022) fishery products fish frauds Multiplex PCR COI gene Nutrition. Foods and food supply TX341-641 article 2022 ftdoajarticles https://doi.org/10.5219/1777 2022-12-30T20:23:00Z This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For this study, a local market survey on fish was carried out to evaluate the application of labelling laws and to detect fraudulent actions using the developed PCR protocols. Ten specimens of each fish species of interest were obtained from wholesale fishery plants and were utilized for the protocol development. DNA was extracted from the individual samples and quantified. DNA isolates were subjected to end-point PCR and the PCR products were sequenced. For the identification of fish species, novel species-specific primers were developed by the program "Primer Express 3.0" and by the software “Primer-BLAST” to amplify fragments of 200 bp, 250 bp, 300 and 562 bp, 350 bp, 400 bp and 522 bp within the COI gene for M. merluccius, L. niloticus, G. morhua, R. pretiosus, P. hypophthalmus, Epinephelus spp., respectively. Single PCR was performed using DNA isolates and developed primers for each fish species of interest. After sequencing, the isolates were compared with the selected sequences of the COI gene and showed a similarity ranging from 99 to 100%. Among 43 samples obtained for the survey, 19 (44.2%) were mislabelled, with 18 (41.9%) mislabelled samples from local fisheries and fish marketplaces and 1 (2.32%) from hypermarket stores. Among fish samples purchased at local fisheries and fish marketplaces, fraudulent actions were observed more frequently in fish slices (100%) than fish fillets (65%). Regarding fish fillets, out of four samples labelled as grouper, three were L. niloticus and one P. hypophthalmus. Two fillets marketed as cod were substituted with L. niloticus. Five samples labelled as “fillet” and two samples labelled as “perch” were identified as P. hypophthalmus. Regarding fish slices, all samples marketed as ... Article in Journal/Newspaper Gadus morhua Directory of Open Access Journals: DOAJ Articles Potravinarstvo Slovak Journal of Food Sciences 16 656 669 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fishery products fish frauds Multiplex PCR COI gene Nutrition. Foods and food supply TX341-641 |
spellingShingle |
fishery products fish frauds Multiplex PCR COI gene Nutrition. Foods and food supply TX341-641 Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
topic_facet |
fishery products fish frauds Multiplex PCR COI gene Nutrition. Foods and food supply TX341-641 |
description |
This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For this study, a local market survey on fish was carried out to evaluate the application of labelling laws and to detect fraudulent actions using the developed PCR protocols. Ten specimens of each fish species of interest were obtained from wholesale fishery plants and were utilized for the protocol development. DNA was extracted from the individual samples and quantified. DNA isolates were subjected to end-point PCR and the PCR products were sequenced. For the identification of fish species, novel species-specific primers were developed by the program "Primer Express 3.0" and by the software “Primer-BLAST” to amplify fragments of 200 bp, 250 bp, 300 and 562 bp, 350 bp, 400 bp and 522 bp within the COI gene for M. merluccius, L. niloticus, G. morhua, R. pretiosus, P. hypophthalmus, Epinephelus spp., respectively. Single PCR was performed using DNA isolates and developed primers for each fish species of interest. After sequencing, the isolates were compared with the selected sequences of the COI gene and showed a similarity ranging from 99 to 100%. Among 43 samples obtained for the survey, 19 (44.2%) were mislabelled, with 18 (41.9%) mislabelled samples from local fisheries and fish marketplaces and 1 (2.32%) from hypermarket stores. Among fish samples purchased at local fisheries and fish marketplaces, fraudulent actions were observed more frequently in fish slices (100%) than fish fillets (65%). Regarding fish fillets, out of four samples labelled as grouper, three were L. niloticus and one P. hypophthalmus. Two fillets marketed as cod were substituted with L. niloticus. Five samples labelled as “fillet” and two samples labelled as “perch” were identified as P. hypophthalmus. Regarding fish slices, all samples marketed as ... |
format |
Article in Journal/Newspaper |
author |
Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno |
author_facet |
Ashraf Ali Pietro Di Taranto Antonio Parisi Laura Del Sambro Antonio Iannacci Donatella Belluscio Daniela Paola Debernardis Giovanni Normanno |
author_sort |
Ashraf Ali |
title |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_short |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_full |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_fullStr |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_full_unstemmed |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_sort |
fish market survey using a novel pcr-sequencing-based protocols for the identification of commercial significant fish species |
publisher |
HACCP Consulting |
publishDate |
2022 |
url |
https://doi.org/10.5219/1777 https://doaj.org/article/3bb542c0b1824287af8eab45f71b251b |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Potravinarstvo, Vol 16 (2022) |
op_relation |
https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 https://doaj.org/toc/1337-0960 doi:10.5219/1777 1337-0960 https://doaj.org/article/3bb542c0b1824287af8eab45f71b251b |
op_doi |
https://doi.org/10.5219/1777 |
container_title |
Potravinarstvo Slovak Journal of Food Sciences |
container_volume |
16 |
container_start_page |
656 |
op_container_end_page |
669 |
_version_ |
1766005797739823104 |