Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis
Objective: To evaluate the cytotoxic and antibacterial effect of Bixa orellana L. (B. orellana) (achiote) methanol extract against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods: Two methanol extracts of B. orellana were prepared in vit...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2016
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Online Access: | https://doi.org/10.1016/j.apjtb.2016.03.005 https://doaj.org/article/3b99b9d9130049278cf5de68a2985924 |
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ftdoajarticles:oai:doaj.org/article:3b99b9d9130049278cf5de68a2985924 2023-05-15T15:11:12+02:00 Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis Dyanne Medina-Flores Gabriela Ulloa-Urizar Rosella Camere-Colarossi Stefany Caballero-García Frank Mayta-Tovalino Juana del Valle-Mendoza 2016-05-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.03.005 https://doaj.org/article/3b99b9d9130049278cf5de68a2985924 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115309485 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.03.005 https://doaj.org/article/3b99b9d9130049278cf5de68a2985924 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 5, Pp 400-403 (2016) Antibacterial effect Medicinal plants Bixa orellana L Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.03.005 2022-12-31T03:44:47Z Objective: To evaluate the cytotoxic and antibacterial effect of Bixa orellana L. (B. orellana) (achiote) methanol extract against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods: Two methanol extracts of B. orellana were prepared in vitro, from the seeds and leaves. The antibacterial activity of extracts against S. mutans and S. sanguinis was evaluated using the cup-plate agar diffusion method. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxic activity was determinated by using the cell line MDCK. Results: A stronger antibacterial effect was observed with the leaves methanolic extract with an inhibition zone of (19.97 ± 1.31) mm against S. mutans and (19.97 ± 1.26) mm against S. sanguinis. The methanolic extract of the seeds had an activity of (15.11 ± 1.03) mm and (16.15 ± 2.15) mm against S. mutans and S. sanguinis, respectively. The MIC of the leaf and the seed extracts against S. sanguinis was 62.5 and 125 μg/mL, respectively, and the MIC of the leaf extract against S. mutans was 62.5 μg/mL, and for the seed extract it was 31.25 μg/mL. The 50% cytotoxic concentration was 366.45 and 325.05 μg/mL for the leaves and seeds extracts, respectively. Conclusions: The experimental findings demonstrated the antibacterial effect of the methanolic extract of B. orellana (achiote) on S. mutans and S. sanguinis. The extract of this plant is cytotoxic at high concentrations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 5 400 403 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Antibacterial effect Medicinal plants Bixa orellana L Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antibacterial effect Medicinal plants Bixa orellana L Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Dyanne Medina-Flores Gabriela Ulloa-Urizar Rosella Camere-Colarossi Stefany Caballero-García Frank Mayta-Tovalino Juana del Valle-Mendoza Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis |
topic_facet |
Antibacterial effect Medicinal plants Bixa orellana L Cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the cytotoxic and antibacterial effect of Bixa orellana L. (B. orellana) (achiote) methanol extract against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods: Two methanol extracts of B. orellana were prepared in vitro, from the seeds and leaves. The antibacterial activity of extracts against S. mutans and S. sanguinis was evaluated using the cup-plate agar diffusion method. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxic activity was determinated by using the cell line MDCK. Results: A stronger antibacterial effect was observed with the leaves methanolic extract with an inhibition zone of (19.97 ± 1.31) mm against S. mutans and (19.97 ± 1.26) mm against S. sanguinis. The methanolic extract of the seeds had an activity of (15.11 ± 1.03) mm and (16.15 ± 2.15) mm against S. mutans and S. sanguinis, respectively. The MIC of the leaf and the seed extracts against S. sanguinis was 62.5 and 125 μg/mL, respectively, and the MIC of the leaf extract against S. mutans was 62.5 μg/mL, and for the seed extract it was 31.25 μg/mL. The 50% cytotoxic concentration was 366.45 and 325.05 μg/mL for the leaves and seeds extracts, respectively. Conclusions: The experimental findings demonstrated the antibacterial effect of the methanolic extract of B. orellana (achiote) on S. mutans and S. sanguinis. The extract of this plant is cytotoxic at high concentrations. |
format |
Article in Journal/Newspaper |
author |
Dyanne Medina-Flores Gabriela Ulloa-Urizar Rosella Camere-Colarossi Stefany Caballero-García Frank Mayta-Tovalino Juana del Valle-Mendoza |
author_facet |
Dyanne Medina-Flores Gabriela Ulloa-Urizar Rosella Camere-Colarossi Stefany Caballero-García Frank Mayta-Tovalino Juana del Valle-Mendoza |
author_sort |
Dyanne Medina-Flores |
title |
Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis |
title_short |
Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis |
title_full |
Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis |
title_fullStr |
Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis |
title_full_unstemmed |
Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis |
title_sort |
antibacterial activity of bixa orellana l. (achiote) against streptococcus mutans and streptococcus sanguinis |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.03.005 https://doaj.org/article/3b99b9d9130049278cf5de68a2985924 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 5, Pp 400-403 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115309485 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.03.005 https://doaj.org/article/3b99b9d9130049278cf5de68a2985924 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.03.005 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
5 |
container_start_page |
400 |
op_container_end_page |
403 |
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1766342102005841920 |