Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders
Introduction: Native Hawaiian and other Pacific Islander (NHPI) populations experience higher rates of immunometabolic diseases compared to other racial-ethnic groups in Hawaii. As annual NHPI mortality rates for suicide and type 2 diabetes mellitus (T2DM) exceed those of the state as a whole, under...
Published in: | Frontiers in Genetics |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fgene.2023.1125217 https://doaj.org/article/3b6aa1db38e44054950b7776d690c5cc |
id |
ftdoajarticles:oai:doaj.org/article:3b6aa1db38e44054950b7776d690c5cc |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3b6aa1db38e44054950b7776d690c5cc 2023-06-11T04:11:18+02:00 Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders Celyna Y. Becerra Riley K. Wells Braden P. Kunihiro Rosa H. Lee Lesley Umeda Nina P. Allan Noelle C. Rubas Trevor A. McCracken Chandler K. L. Nunokawa Ming-Hao Lee Felix Gerard S. Pidlaoan Krit Phankitnirondorn Christian K. Dye Brennan Y Yamamoto Rafael Peres Ruben Juarez Alika K. Maunakea 2023-04-01T00:00:00Z https://doi.org/10.3389/fgene.2023.1125217 https://doaj.org/article/3b6aa1db38e44054950b7776d690c5cc EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fgene.2023.1125217/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2023.1125217 https://doaj.org/article/3b6aa1db38e44054950b7776d690c5cc Frontiers in Genetics, Vol 14 (2023) health disparities depressive symptoms epigenetic aging self-esteem immune system gut microbiome Genetics QH426-470 article 2023 ftdoajarticles https://doi.org/10.3389/fgene.2023.1125217 2023-04-23T00:34:10Z Introduction: Native Hawaiian and other Pacific Islander (NHPI) populations experience higher rates of immunometabolic diseases compared to other racial-ethnic groups in Hawaii. As annual NHPI mortality rates for suicide and type 2 diabetes mellitus (T2DM) exceed those of the state as a whole, understanding the social and biological mechanisms underlying these disparities are urgently needed to enable preventive strategies.Methods: A community-based approach was used to investigate the immunoepigenetic-gut microbiome axis in an NHPI-enriched cohort of Oahu residents (N = 68). Self-esteem (SE) data was collected using a modified Rosenberg self-esteem (SE) assessment as a proxy measure for mental wellbeing in consideration for cultural competency. T2DM status was evaluated using point-of-care A1c (%) tests. Stool samples were collected for 16s-based metagenomic sequencing analyses. Plasma from blood samples were isolated by density-gradient centrifugation. Peripheral blood mononuclear cells (PBMCs) were collected from the same samples and enriched for monocytes using negative selection techniques. Flow-cytometry was used for immunoprofiling assays. Monocyte DNA was extracted for Illumina EPIC array-based methylation analysis.Results: Compared to individuals with normal SE (NSE), those with low SE (LSE) exhibited significantly higher plasma concentrations (pg/ml) of proinflammatory cytokines IL-8 (p = 0.051) and TNF-α (p = 0.011). Metagenomic analysis revealed that the relative abundance (%) of specific gut bacteria significantly differed between SE groups - some of which directly correlated with SE scores. Gene ontology analysis revealed that 104 significantly differentially methylated loci (DML) between SE groups were preferentially located at genes involved in immunometabolic processes. Horvath clock analyses indicated epigenetic age (Epi-Age) deceleration in individuals with LSE and acceleration in individuals with NSE (p = 0.042), yet was not reproduced by other clocks.Discussion: These data reveal novel ... Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles Horvath ENVELOPE(-67.137,-67.137,-66.309,-66.309) Pacific Frontiers in Genetics 14 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
health disparities depressive symptoms epigenetic aging self-esteem immune system gut microbiome Genetics QH426-470 |
spellingShingle |
health disparities depressive symptoms epigenetic aging self-esteem immune system gut microbiome Genetics QH426-470 Celyna Y. Becerra Riley K. Wells Braden P. Kunihiro Rosa H. Lee Lesley Umeda Nina P. Allan Noelle C. Rubas Trevor A. McCracken Chandler K. L. Nunokawa Ming-Hao Lee Felix Gerard S. Pidlaoan Krit Phankitnirondorn Christian K. Dye Brennan Y Yamamoto Rafael Peres Ruben Juarez Alika K. Maunakea Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders |
topic_facet |
health disparities depressive symptoms epigenetic aging self-esteem immune system gut microbiome Genetics QH426-470 |
description |
Introduction: Native Hawaiian and other Pacific Islander (NHPI) populations experience higher rates of immunometabolic diseases compared to other racial-ethnic groups in Hawaii. As annual NHPI mortality rates for suicide and type 2 diabetes mellitus (T2DM) exceed those of the state as a whole, understanding the social and biological mechanisms underlying these disparities are urgently needed to enable preventive strategies.Methods: A community-based approach was used to investigate the immunoepigenetic-gut microbiome axis in an NHPI-enriched cohort of Oahu residents (N = 68). Self-esteem (SE) data was collected using a modified Rosenberg self-esteem (SE) assessment as a proxy measure for mental wellbeing in consideration for cultural competency. T2DM status was evaluated using point-of-care A1c (%) tests. Stool samples were collected for 16s-based metagenomic sequencing analyses. Plasma from blood samples were isolated by density-gradient centrifugation. Peripheral blood mononuclear cells (PBMCs) were collected from the same samples and enriched for monocytes using negative selection techniques. Flow-cytometry was used for immunoprofiling assays. Monocyte DNA was extracted for Illumina EPIC array-based methylation analysis.Results: Compared to individuals with normal SE (NSE), those with low SE (LSE) exhibited significantly higher plasma concentrations (pg/ml) of proinflammatory cytokines IL-8 (p = 0.051) and TNF-α (p = 0.011). Metagenomic analysis revealed that the relative abundance (%) of specific gut bacteria significantly differed between SE groups - some of which directly correlated with SE scores. Gene ontology analysis revealed that 104 significantly differentially methylated loci (DML) between SE groups were preferentially located at genes involved in immunometabolic processes. Horvath clock analyses indicated epigenetic age (Epi-Age) deceleration in individuals with LSE and acceleration in individuals with NSE (p = 0.042), yet was not reproduced by other clocks.Discussion: These data reveal novel ... |
format |
Article in Journal/Newspaper |
author |
Celyna Y. Becerra Riley K. Wells Braden P. Kunihiro Rosa H. Lee Lesley Umeda Nina P. Allan Noelle C. Rubas Trevor A. McCracken Chandler K. L. Nunokawa Ming-Hao Lee Felix Gerard S. Pidlaoan Krit Phankitnirondorn Christian K. Dye Brennan Y Yamamoto Rafael Peres Ruben Juarez Alika K. Maunakea |
author_facet |
Celyna Y. Becerra Riley K. Wells Braden P. Kunihiro Rosa H. Lee Lesley Umeda Nina P. Allan Noelle C. Rubas Trevor A. McCracken Chandler K. L. Nunokawa Ming-Hao Lee Felix Gerard S. Pidlaoan Krit Phankitnirondorn Christian K. Dye Brennan Y Yamamoto Rafael Peres Ruben Juarez Alika K. Maunakea |
author_sort |
Celyna Y. Becerra |
title |
Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders |
title_short |
Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders |
title_full |
Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders |
title_fullStr |
Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders |
title_full_unstemmed |
Examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among Native Hawaiians and other Pacific Islanders |
title_sort |
examining the immunoepigenetic-gut microbiome axis in the context of self-esteem among native hawaiians and other pacific islanders |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fgene.2023.1125217 https://doaj.org/article/3b6aa1db38e44054950b7776d690c5cc |
long_lat |
ENVELOPE(-67.137,-67.137,-66.309,-66.309) |
geographic |
Horvath Pacific |
geographic_facet |
Horvath Pacific |
genre |
DML |
genre_facet |
DML |
op_source |
Frontiers in Genetics, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fgene.2023.1125217/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2023.1125217 https://doaj.org/article/3b6aa1db38e44054950b7776d690c5cc |
op_doi |
https://doi.org/10.3389/fgene.2023.1125217 |
container_title |
Frontiers in Genetics |
container_volume |
14 |
_version_ |
1768386260429176832 |