Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction.
BACKGROUND: Trichinellosis is a typical food-borne zoonotic disease which is epidemic worldwide and the nematode Trichinella spiralis is the main pathogen. The life cycle of T. spiralis contains three developmental stages, i.e. adult worms, new borne larva (new borne L1 larva) and muscular larva (in...
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ftdoajarticles:oai:doaj.org/article:3b4b30ff2bc546968b22662fe78f7c43 2023-05-15T15:15:27+02:00 Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. Xiaolei Liu Yanxia Song Ning Jiang Jielin Wang Bin Tang Huijun Lu Shuai Peng Zhiguang Chang Yizhi Tang Jigang Yin Mingyuan Liu Yan Tan Qijun Chen 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001794 https://doaj.org/article/3b4b30ff2bc546968b22662fe78f7c43 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3429391?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0001794 1935-2727 1935-2735 https://doaj.org/article/3b4b30ff2bc546968b22662fe78f7c43 PLoS Neglected Tropical Diseases, Vol 6, Iss 8, p e1794 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001794 2022-12-31T09:24:23Z BACKGROUND: Trichinellosis is a typical food-borne zoonotic disease which is epidemic worldwide and the nematode Trichinella spiralis is the main pathogen. The life cycle of T. spiralis contains three developmental stages, i.e. adult worms, new borne larva (new borne L1 larva) and muscular larva (infective L1 larva). Stage-specific gene expression in the parasites has been investigated with various immunological and cDNA cloning approaches, whereas the genome-wide transcriptome and expression features of the parasite have been largely unknown. The availability of the genome sequence information of T. spiralis has made it possible to deeply dissect parasite biology in association with global gene expression and pathogenesis. METHODOLOGY AND PRINCIPAL FINDINGS: In this study, we analyzed the global gene expression patterns in the three developmental stages of T. spiralis using digital gene expression (DGE) analysis. Almost 15 million sequence tags were generated with the Illumina RNA-seq technology, producing expression data for more than 9,000 genes, covering 65% of the genome. The transcriptome analysis revealed thousands of differentially expressed genes within the genome, and importantly, a panel of genes encoding functional proteins associated with parasite invasion and immuno-modulation were identified. More than 45% of the genes were found to be transcribed from both strands, indicating the importance of RNA-mediated gene regulation in the development of the parasite. Further, based on gene ontological analysis, over 3000 genes were functionally categorized and biological pathways in the three life cycle stage were elucidated. CONCLUSIONS AND SIGNIFICANCE: The global transcriptome of T. spiralis in three developmental stages has been profiled, and most gene activity in the genome was found to be developmentally regulated. Many metabolic and biological pathways have been revealed. The findings of the differential expression of several protein families facilitate understanding of the molecular mechanisms of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 8 e1794 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiaolei Liu Yanxia Song Ning Jiang Jielin Wang Bin Tang Huijun Lu Shuai Peng Zhiguang Chang Yizhi Tang Jigang Yin Mingyuan Liu Yan Tan Qijun Chen Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Trichinellosis is a typical food-borne zoonotic disease which is epidemic worldwide and the nematode Trichinella spiralis is the main pathogen. The life cycle of T. spiralis contains three developmental stages, i.e. adult worms, new borne larva (new borne L1 larva) and muscular larva (infective L1 larva). Stage-specific gene expression in the parasites has been investigated with various immunological and cDNA cloning approaches, whereas the genome-wide transcriptome and expression features of the parasite have been largely unknown. The availability of the genome sequence information of T. spiralis has made it possible to deeply dissect parasite biology in association with global gene expression and pathogenesis. METHODOLOGY AND PRINCIPAL FINDINGS: In this study, we analyzed the global gene expression patterns in the three developmental stages of T. spiralis using digital gene expression (DGE) analysis. Almost 15 million sequence tags were generated with the Illumina RNA-seq technology, producing expression data for more than 9,000 genes, covering 65% of the genome. The transcriptome analysis revealed thousands of differentially expressed genes within the genome, and importantly, a panel of genes encoding functional proteins associated with parasite invasion and immuno-modulation were identified. More than 45% of the genes were found to be transcribed from both strands, indicating the importance of RNA-mediated gene regulation in the development of the parasite. Further, based on gene ontological analysis, over 3000 genes were functionally categorized and biological pathways in the three life cycle stage were elucidated. CONCLUSIONS AND SIGNIFICANCE: The global transcriptome of T. spiralis in three developmental stages has been profiled, and most gene activity in the genome was found to be developmentally regulated. Many metabolic and biological pathways have been revealed. The findings of the differential expression of several protein families facilitate understanding of the molecular mechanisms of ... |
format |
Article in Journal/Newspaper |
author |
Xiaolei Liu Yanxia Song Ning Jiang Jielin Wang Bin Tang Huijun Lu Shuai Peng Zhiguang Chang Yizhi Tang Jigang Yin Mingyuan Liu Yan Tan Qijun Chen |
author_facet |
Xiaolei Liu Yanxia Song Ning Jiang Jielin Wang Bin Tang Huijun Lu Shuai Peng Zhiguang Chang Yizhi Tang Jigang Yin Mingyuan Liu Yan Tan Qijun Chen |
author_sort |
Xiaolei Liu |
title |
Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. |
title_short |
Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. |
title_full |
Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. |
title_fullStr |
Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. |
title_full_unstemmed |
Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction. |
title_sort |
global gene expression analysis of the zoonotic parasite trichinella spiralis revealed novel genes in host parasite interaction. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001794 https://doaj.org/article/3b4b30ff2bc546968b22662fe78f7c43 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 8, p e1794 (2012) |
op_relation |
http://europepmc.org/articles/PMC3429391?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0001794 1935-2727 1935-2735 https://doaj.org/article/3b4b30ff2bc546968b22662fe78f7c43 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001794 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
8 |
container_start_page |
e1794 |
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