Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent
Abstract Background Genetic polymorphism is an inevitable component of a complex organism especially in multistage infectious organisms such as malaria parasites. Understanding the population genetic structure of the parasites would provide valuable information for effective malaria control strategi...
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ftdoajarticles:oai:doaj.org/article:3b2e4ac000bb4931b0fd91b52c612569 2023-05-15T15:13:28+02:00 Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent Singh Neeru Das Manoj K Eapen Alex Kumar Ashwini Yadav Rajpal S Adak Tridibes Verma Anju Prajapati Surendra K Sharma Surya K Rizvi Moshahid A Dash Aditya P Joshi Hema 2006-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-90 https://doaj.org/article/3b2e4ac000bb4931b0fd91b52c612569 EN eng BMC http://www.malariajournal.com/content/5/1/90 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-90 1475-2875 https://doaj.org/article/3b2e4ac000bb4931b0fd91b52c612569 Malaria Journal, Vol 5, Iss 1, p 90 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-90 2022-12-31T01:27:44Z Abstract Background Genetic polymorphism is an inevitable component of a complex organism especially in multistage infectious organisms such as malaria parasites. Understanding the population genetic structure of the parasites would provide valuable information for effective malaria control strategies. Recently, the development of molecular tools like PCR has made analysis of field samples possible and easier and research on Plasmodium vivax has also been strengthened. Not many reports are available on the genetic polymorphism of P. vivax from the Indian sub-continent. This study evaluates the extent of diversity in field isolates of India with respect to Pvgam-1 . Methods A study was designed to assess the diversity of Pvgam-1 among field isolates from India, using a nested PCR assay. Field isolates were collected from different regions of the country and the observed variability was confirmed by sequencing data. Results Both Belem and Chesson type alleles were present either exclusively or in mixed form among isolates of all 10 study sites. The Belem type allele was predominant, occurring in 67% of isolates. The proportion of isolates showing the mixed form (both Belem and Chesson type alleles occurring together in the same isolate) was about 13 overall (up to 38.5% in some isolates). Sequencing of the PCR-amplified Belem and Chesson type alleles confirmed the PCR results. Among the 10 study sequences, 11 polymorphic sites and four singleton variations were observed. All the nucleotide substitutions were non-synonymous. Conclusion Study shows limited diversity of Pvgam-1 marker in Indian isolates with well representation of both Belem and Chesson type alleles. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Gam ENVELOPE(-57.955,-57.955,-61.923,-61.923) Malaria Journal 5 1 90 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Singh Neeru Das Manoj K Eapen Alex Kumar Ashwini Yadav Rajpal S Adak Tridibes Verma Anju Prajapati Surendra K Sharma Surya K Rizvi Moshahid A Dash Aditya P Joshi Hema Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Genetic polymorphism is an inevitable component of a complex organism especially in multistage infectious organisms such as malaria parasites. Understanding the population genetic structure of the parasites would provide valuable information for effective malaria control strategies. Recently, the development of molecular tools like PCR has made analysis of field samples possible and easier and research on Plasmodium vivax has also been strengthened. Not many reports are available on the genetic polymorphism of P. vivax from the Indian sub-continent. This study evaluates the extent of diversity in field isolates of India with respect to Pvgam-1 . Methods A study was designed to assess the diversity of Pvgam-1 among field isolates from India, using a nested PCR assay. Field isolates were collected from different regions of the country and the observed variability was confirmed by sequencing data. Results Both Belem and Chesson type alleles were present either exclusively or in mixed form among isolates of all 10 study sites. The Belem type allele was predominant, occurring in 67% of isolates. The proportion of isolates showing the mixed form (both Belem and Chesson type alleles occurring together in the same isolate) was about 13 overall (up to 38.5% in some isolates). Sequencing of the PCR-amplified Belem and Chesson type alleles confirmed the PCR results. Among the 10 study sequences, 11 polymorphic sites and four singleton variations were observed. All the nucleotide substitutions were non-synonymous. Conclusion Study shows limited diversity of Pvgam-1 marker in Indian isolates with well representation of both Belem and Chesson type alleles. |
format |
Article in Journal/Newspaper |
author |
Singh Neeru Das Manoj K Eapen Alex Kumar Ashwini Yadav Rajpal S Adak Tridibes Verma Anju Prajapati Surendra K Sharma Surya K Rizvi Moshahid A Dash Aditya P Joshi Hema |
author_facet |
Singh Neeru Das Manoj K Eapen Alex Kumar Ashwini Yadav Rajpal S Adak Tridibes Verma Anju Prajapati Surendra K Sharma Surya K Rizvi Moshahid A Dash Aditya P Joshi Hema |
author_sort |
Singh Neeru |
title |
Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent |
title_short |
Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent |
title_full |
Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent |
title_fullStr |
Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent |
title_full_unstemmed |
Allelic dimorphism of Plasmodium vivax gam-1 in the Indian subcontinent |
title_sort |
allelic dimorphism of plasmodium vivax gam-1 in the indian subcontinent |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-90 https://doaj.org/article/3b2e4ac000bb4931b0fd91b52c612569 |
long_lat |
ENVELOPE(-57.955,-57.955,-61.923,-61.923) |
geographic |
Arctic Indian Gam |
geographic_facet |
Arctic Indian Gam |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 90 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/90 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-90 1475-2875 https://doaj.org/article/3b2e4ac000bb4931b0fd91b52c612569 |
op_doi |
https://doi.org/10.1186/1475-2875-5-90 |
container_title |
Malaria Journal |
container_volume |
5 |
container_issue |
1 |
container_start_page |
90 |
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1766344026935525376 |