Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms.
Development of gastric diseases such as gastritis, peptic ulcer and gastric cancer is often associated with several biotic and abiotic factors. Helicobacter pylori infection is such a well-known biotic factor. However, not all H. pylori-infected individuals develop gastric diseases and not all indiv...
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ftdoajarticles:oai:doaj.org/article:3b2e1cd234344a489a93736134644f56 2023-05-15T15:16:32+02:00 Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. Deepak Chouhan T Barani Devi Santanu Chattopadhyay Sanjai Dharmaseelan Gopinath Balakrish Nair Krishnadas Devadas Madhavan Radhakrishna Pillai 2019-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007799 https://doaj.org/article/3b2e1cd234344a489a93736134644f56 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007799 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007799 https://doaj.org/article/3b2e1cd234344a489a93736134644f56 PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007799 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007799 2022-12-31T11:45:09Z Development of gastric diseases such as gastritis, peptic ulcer and gastric cancer is often associated with several biotic and abiotic factors. Helicobacter pylori infection is such a well-known biotic factor. However, not all H. pylori-infected individuals develop gastric diseases and not all individuals with gastric diseases are infected with H. pylori. Therefore, it is possible that other gastric bacteria may contribute to the formation and progression of gastric disease. The aim of this study was to isolate prevalent gastric bacteria under microaerobic condition and identify them by 16S rRNA gene sequence analysis. Analysis of gastric biopsies showed infection of Mycobacterium abscessus (phylum Actinobacteria) to be highly prevalent in the stomachs of subjects included. Our data show that of 129 (67 male and 62 female) patients with gastric symptoms, 96 (51 male and 45 female) showed the presence of M. abscessus in stomach tissues. Infection of M. abscessus in gastric epithelium was further confirmed by imaging with acid fast staining, immunohistochemistry and immunofluorescence. Our imaging data strongly suggested that M. abscessus is an intracellular colonizer residing inside the gastric epithelial cells rather than in macrophages. Additionally, phylogenetic analysis of the mycobacterial hsp65 gene showed that the nearest match to the M. abscessus strains isolated from our study subjects is the M. abscessus strain ATCC 19977. Surprisingly, the subjects studied, the prevalence of M. abscessus infection in stomach is even higher than the prevalence of H. pylori infection. This, to the best of our knowledge, is the first study showing the colonization of M. abscessus in human gastric mucosa among patients with various gastric symptoms. This study could provide usher in a new opportunity to understand the role of less studied gastric bacteria in the development of gastric diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 11 e0007799 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Deepak Chouhan T Barani Devi Santanu Chattopadhyay Sanjai Dharmaseelan Gopinath Balakrish Nair Krishnadas Devadas Madhavan Radhakrishna Pillai Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Development of gastric diseases such as gastritis, peptic ulcer and gastric cancer is often associated with several biotic and abiotic factors. Helicobacter pylori infection is such a well-known biotic factor. However, not all H. pylori-infected individuals develop gastric diseases and not all individuals with gastric diseases are infected with H. pylori. Therefore, it is possible that other gastric bacteria may contribute to the formation and progression of gastric disease. The aim of this study was to isolate prevalent gastric bacteria under microaerobic condition and identify them by 16S rRNA gene sequence analysis. Analysis of gastric biopsies showed infection of Mycobacterium abscessus (phylum Actinobacteria) to be highly prevalent in the stomachs of subjects included. Our data show that of 129 (67 male and 62 female) patients with gastric symptoms, 96 (51 male and 45 female) showed the presence of M. abscessus in stomach tissues. Infection of M. abscessus in gastric epithelium was further confirmed by imaging with acid fast staining, immunohistochemistry and immunofluorescence. Our imaging data strongly suggested that M. abscessus is an intracellular colonizer residing inside the gastric epithelial cells rather than in macrophages. Additionally, phylogenetic analysis of the mycobacterial hsp65 gene showed that the nearest match to the M. abscessus strains isolated from our study subjects is the M. abscessus strain ATCC 19977. Surprisingly, the subjects studied, the prevalence of M. abscessus infection in stomach is even higher than the prevalence of H. pylori infection. This, to the best of our knowledge, is the first study showing the colonization of M. abscessus in human gastric mucosa among patients with various gastric symptoms. This study could provide usher in a new opportunity to understand the role of less studied gastric bacteria in the development of gastric diseases. |
format |
Article in Journal/Newspaper |
author |
Deepak Chouhan T Barani Devi Santanu Chattopadhyay Sanjai Dharmaseelan Gopinath Balakrish Nair Krishnadas Devadas Madhavan Radhakrishna Pillai |
author_facet |
Deepak Chouhan T Barani Devi Santanu Chattopadhyay Sanjai Dharmaseelan Gopinath Balakrish Nair Krishnadas Devadas Madhavan Radhakrishna Pillai |
author_sort |
Deepak Chouhan |
title |
Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
title_short |
Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
title_full |
Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
title_fullStr |
Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
title_full_unstemmed |
Mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
title_sort |
mycobacterium abscessus infection in the stomach of patients with various gastric symptoms. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007799 https://doaj.org/article/3b2e1cd234344a489a93736134644f56 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007799 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007799 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007799 https://doaj.org/article/3b2e1cd234344a489a93736134644f56 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007799 |
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PLOS Neglected Tropical Diseases |
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11 |
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e0007799 |
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