Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros
To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cu...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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ftdoajarticles:oai:doaj.org/article:3b2ade7e9b00437687a118c4abafdfb8 2023-05-15T15:17:55+02:00 Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros Carolina V. Pannuti Maria Lúcia S.G. Jorge Cláudia Biasutti Esper G. Kallás Aluisio A.C. Segurado 2004-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822004000400008 https://doaj.org/article/3b2ade7e9b00437687a118c4abafdfb8 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822004000400008 0037-8682 1678-9849 https://doaj.org/article/3b2ade7e9b00437687a118c4abafdfb8 Revista da Sociedade Brasileira de Medicina Tropical, Vol 37, Iss 4, Pp 329-332 (2004) HTLV-I Cultura Linhagens celulares Brasil Culture Cell line Brazil Arctic medicine. Tropical medicine RC955-962 article 2004 ftdoajarticles https://doi.org/10.1590/S0037-86822004000400008 2022-12-31T02:02:45Z To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests. Para investigar a epidemiologia e patogênese da infecção por HTLV-I seguimos coorte de portadores dessa retrovirose no HC-FMUSP desde 1991. Este estudo descreve o estabelecimento de linhagens celulares a partir de células mononucleares periféricas (CMP) de indivíduos infectados. As CMP foram cultivadas com as de doador soronegativo, verificando-se após 90 dias evidência morfológica de transformação celular com detecção de células multinucleadas com núcleos cerebriformes. Demonstrou-se integração do DNA proviral e expressão in vitro de antígenos virais pela PCR e imunofluorescência. As linhagens celulares transformadas foram mantidas por 240 dias, com retirada gradual de IL-2 exógena. A imunofenotipagem por citometria de fluxo revelou ativação celular. O estabelecimento de linhagens celulares infectadas por HTLV-I a partir de CMP ex-vivo é exeqüível e pode ser útil na investigação de alterações fenotípicas linfocitárias e dos mecanismos de proliferação celular induzida por esse ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 37 4 329 332 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
HTLV-I Cultura Linhagens celulares Brasil Culture Cell line Brazil Arctic medicine. Tropical medicine RC955-962 |
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HTLV-I Cultura Linhagens celulares Brasil Culture Cell line Brazil Arctic medicine. Tropical medicine RC955-962 Carolina V. Pannuti Maria Lúcia S.G. Jorge Cláudia Biasutti Esper G. Kallás Aluisio A.C. Segurado Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros |
topic_facet |
HTLV-I Cultura Linhagens celulares Brasil Culture Cell line Brazil Arctic medicine. Tropical medicine RC955-962 |
description |
To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests. Para investigar a epidemiologia e patogênese da infecção por HTLV-I seguimos coorte de portadores dessa retrovirose no HC-FMUSP desde 1991. Este estudo descreve o estabelecimento de linhagens celulares a partir de células mononucleares periféricas (CMP) de indivíduos infectados. As CMP foram cultivadas com as de doador soronegativo, verificando-se após 90 dias evidência morfológica de transformação celular com detecção de células multinucleadas com núcleos cerebriformes. Demonstrou-se integração do DNA proviral e expressão in vitro de antígenos virais pela PCR e imunofluorescência. As linhagens celulares transformadas foram mantidas por 240 dias, com retirada gradual de IL-2 exógena. A imunofenotipagem por citometria de fluxo revelou ativação celular. O estabelecimento de linhagens celulares infectadas por HTLV-I a partir de CMP ex-vivo é exeqüível e pode ser útil na investigação de alterações fenotípicas linfocitárias e dos mecanismos de proliferação celular induzida por esse ... |
format |
Article in Journal/Newspaper |
author |
Carolina V. Pannuti Maria Lúcia S.G. Jorge Cláudia Biasutti Esper G. Kallás Aluisio A.C. Segurado |
author_facet |
Carolina V. Pannuti Maria Lúcia S.G. Jorge Cláudia Biasutti Esper G. Kallás Aluisio A.C. Segurado |
author_sort |
Carolina V. Pannuti |
title |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros |
title_short |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros |
title_full |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros |
title_fullStr |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros |
title_full_unstemmed |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Estabelecimento de linhagens celulares infectadas por HTLV-I a partir de células mononucleares periféricas de pacientes brasileiros |
title_sort |
establishment of htlv-i-infected cell lines from peripheral blood mononuclear cells of brazilian patients estabelecimento de linhagens celulares infectadas por htlv-i a partir de células mononucleares periféricas de pacientes brasileiros |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2004 |
url |
https://doi.org/10.1590/S0037-86822004000400008 https://doaj.org/article/3b2ade7e9b00437687a118c4abafdfb8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 37, Iss 4, Pp 329-332 (2004) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822004000400008 0037-8682 1678-9849 https://doaj.org/article/3b2ade7e9b00437687a118c4abafdfb8 |
op_doi |
https://doi.org/10.1590/S0037-86822004000400008 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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37 |
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