Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae)
Abstract Background Grasslands in the Arctic tundra undergo irreversible degradation due to climatic changes and also over-exploitation and depletion of scarce resources. Comprehensive investigations of cytogenomic structures of valuable Arctic and sub-Arctic grassland species is essential for clari...
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ftdoajarticles:oai:doaj.org/article:3afa85253f5d4465866c2e92428f5775 2023-05-15T14:35:33+02:00 Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) Alexandra V. Amosova Svyatoslav A. Zoshchuk Alexander V. Rodionov Lilit Ghukasyan Tatiana E. Samatadze Elizaveta O. Punina Igor G. Loskutov Olga Yu. Yurkevich Olga V. Muravenko 2019-12-01T00:00:00Z https://doi.org/10.1186/s12863-019-0792-2 https://doaj.org/article/3afa85253f5d4465866c2e92428f5775 EN eng BMC https://doi.org/10.1186/s12863-019-0792-2 https://doaj.org/toc/1471-2156 doi:10.1186/s12863-019-0792-2 1471-2156 https://doaj.org/article/3afa85253f5d4465866c2e92428f5775 BMC Genetics, Vol 20, Iss 1, Pp 1-16 (2019) Genetics QH426-470 article 2019 ftdoajarticles https://doi.org/10.1186/s12863-019-0792-2 2022-12-30T23:52:59Z Abstract Background Grasslands in the Arctic tundra undergo irreversible degradation due to climatic changes and also over-exploitation and depletion of scarce resources. Comprehensive investigations of cytogenomic structures of valuable Arctic and sub-Arctic grassland species is essential for clarifying their genetic peculiarities and phylogenetic relationships, and also successful developing new forage grass cultivars with high levels of adaptation, stable productivity and longevity. We performed molecular cytogenetic characterization of insufficiently studied pasture grass species (Poaceae) from related genera representing two neighboring clades: 1) Deschampsia and Holcus; 2) Alopecurus, Arctagrostis and Beckmannia, which are the primary fodder resources in the Arctic tundra. Results We constructed the integrated schematic maps of distribution of these species in the northern, central and eastern parts of Eurasia based on the currently available data as only scattered data on their occurrence is currently available. The species karyotypes were examined with the use of DAPI-banding, multicolour FISH with 35S rDNA, 5S rDNA and the (GTT)9 microsatellite motif and also sequential rapid multocolour GISH with genomic DNAs of Deschampsia sukatschewii, Deschampsia flexuosa and Holcus lanatus belonging to one of the studied clades. Cytogenomic structures of the species were specified; peculiarities and common features of their genomes were revealed. Different chromosomal rearrangements were detected in Beckmannia syzigachne, Deschampsia cespitosa and D. flexuosa; B chromosomes with distinct DAPI-bands were observed in karyotypes of D. cespitosa and H. lanatus. Conclusions The peculiarities of distribution patterns of the examined chromosomal markers and also presence of common homologous DNA repeats in karyotypes of the studies species allowed us to verify their relationships. The obtained unique data on distribution areas and cytogenomic structures of the valuable Arctic and sub-Arctic pasture species are important ... Article in Journal/Newspaper Arctic Tundra Directory of Open Access Journals: DOAJ Articles Arctic BMC Genetics 20 1 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Genetics QH426-470 |
spellingShingle |
Genetics QH426-470 Alexandra V. Amosova Svyatoslav A. Zoshchuk Alexander V. Rodionov Lilit Ghukasyan Tatiana E. Samatadze Elizaveta O. Punina Igor G. Loskutov Olga Yu. Yurkevich Olga V. Muravenko Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) |
topic_facet |
Genetics QH426-470 |
description |
Abstract Background Grasslands in the Arctic tundra undergo irreversible degradation due to climatic changes and also over-exploitation and depletion of scarce resources. Comprehensive investigations of cytogenomic structures of valuable Arctic and sub-Arctic grassland species is essential for clarifying their genetic peculiarities and phylogenetic relationships, and also successful developing new forage grass cultivars with high levels of adaptation, stable productivity and longevity. We performed molecular cytogenetic characterization of insufficiently studied pasture grass species (Poaceae) from related genera representing two neighboring clades: 1) Deschampsia and Holcus; 2) Alopecurus, Arctagrostis and Beckmannia, which are the primary fodder resources in the Arctic tundra. Results We constructed the integrated schematic maps of distribution of these species in the northern, central and eastern parts of Eurasia based on the currently available data as only scattered data on their occurrence is currently available. The species karyotypes were examined with the use of DAPI-banding, multicolour FISH with 35S rDNA, 5S rDNA and the (GTT)9 microsatellite motif and also sequential rapid multocolour GISH with genomic DNAs of Deschampsia sukatschewii, Deschampsia flexuosa and Holcus lanatus belonging to one of the studied clades. Cytogenomic structures of the species were specified; peculiarities and common features of their genomes were revealed. Different chromosomal rearrangements were detected in Beckmannia syzigachne, Deschampsia cespitosa and D. flexuosa; B chromosomes with distinct DAPI-bands were observed in karyotypes of D. cespitosa and H. lanatus. Conclusions The peculiarities of distribution patterns of the examined chromosomal markers and also presence of common homologous DNA repeats in karyotypes of the studies species allowed us to verify their relationships. The obtained unique data on distribution areas and cytogenomic structures of the valuable Arctic and sub-Arctic pasture species are important ... |
format |
Article in Journal/Newspaper |
author |
Alexandra V. Amosova Svyatoslav A. Zoshchuk Alexander V. Rodionov Lilit Ghukasyan Tatiana E. Samatadze Elizaveta O. Punina Igor G. Loskutov Olga Yu. Yurkevich Olga V. Muravenko |
author_facet |
Alexandra V. Amosova Svyatoslav A. Zoshchuk Alexander V. Rodionov Lilit Ghukasyan Tatiana E. Samatadze Elizaveta O. Punina Igor G. Loskutov Olga Yu. Yurkevich Olga V. Muravenko |
author_sort |
Alexandra V. Amosova |
title |
Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) |
title_short |
Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) |
title_full |
Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) |
title_fullStr |
Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) |
title_full_unstemmed |
Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae) |
title_sort |
molecular cytogenetics of valuable arctic and sub-arctic pasture grass species from the aveneae/poeae tribe complex (poaceae) |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12863-019-0792-2 https://doaj.org/article/3afa85253f5d4465866c2e92428f5775 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
genre_facet |
Arctic Tundra |
op_source |
BMC Genetics, Vol 20, Iss 1, Pp 1-16 (2019) |
op_relation |
https://doi.org/10.1186/s12863-019-0792-2 https://doaj.org/toc/1471-2156 doi:10.1186/s12863-019-0792-2 1471-2156 https://doaj.org/article/3afa85253f5d4465866c2e92428f5775 |
op_doi |
https://doi.org/10.1186/s12863-019-0792-2 |
container_title |
BMC Genetics |
container_volume |
20 |
container_issue |
1 |
_version_ |
1766308361122349056 |