Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.)
Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifu...
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Wolters Kluwer Medknow Publications
2018
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Online Access: | https://doi.org/10.4103/1995-7645.231469 https://doaj.org/article/3ab4ea53d4fc4d0ab5bda3eb116818c9 |
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ftdoajarticles:oai:doaj.org/article:3ab4ea53d4fc4d0ab5bda3eb116818c9 2023-05-15T15:12:26+02:00 Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) Ahlem Ben Sassi Sana Amroussi Malek Besbes Mahjoub Aouni Fethia Skhiri 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.231469 https://doaj.org/article/3ab4ea53d4fc4d0ab5bda3eb116818c9 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=285;epage=291;aulast=Sassi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.231469 https://doaj.org/article/3ab4ea53d4fc4d0ab5bda3eb116818c9 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 4, Pp 285-291 (2018) antioxidant α-glucosidase phenolic content oil chrysanthoglossum trifurcatum Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.231469 2022-12-31T10:02:55Z Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and α -pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC50=0.68 mg/mL) and flowers (IC50=0.67 mg/mL) methanolic extracts and essential oil (IC50=0.72 mg/mL). Findings of α-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC50 of 0.044, 0.045 and 0.049 mg/mL, respectively. Conclusions: Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 4 285 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antioxidant α-glucosidase phenolic content oil chrysanthoglossum trifurcatum Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
antioxidant α-glucosidase phenolic content oil chrysanthoglossum trifurcatum Arctic medicine. Tropical medicine RC955-962 Ahlem Ben Sassi Sana Amroussi Malek Besbes Mahjoub Aouni Fethia Skhiri Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) |
topic_facet |
antioxidant α-glucosidase phenolic content oil chrysanthoglossum trifurcatum Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and α -pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC50=0.68 mg/mL) and flowers (IC50=0.67 mg/mL) methanolic extracts and essential oil (IC50=0.72 mg/mL). Findings of α-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC50 of 0.044, 0.045 and 0.049 mg/mL, respectively. Conclusions: Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes. |
format |
Article in Journal/Newspaper |
author |
Ahlem Ben Sassi Sana Amroussi Malek Besbes Mahjoub Aouni Fethia Skhiri |
author_facet |
Ahlem Ben Sassi Sana Amroussi Malek Besbes Mahjoub Aouni Fethia Skhiri |
author_sort |
Ahlem Ben Sassi |
title |
Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) |
title_short |
Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) |
title_full |
Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) |
title_fullStr |
Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) |
title_full_unstemmed |
Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.) |
title_sort |
antioxidant and α-glucosidase activities and phytochemical constituents of chrysanthoglossum trifurcatum (desf.) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.231469 https://doaj.org/article/3ab4ea53d4fc4d0ab5bda3eb116818c9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 4, Pp 285-291 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=285;epage=291;aulast=Sassi https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.231469 https://doaj.org/article/3ab4ea53d4fc4d0ab5bda3eb116818c9 |
op_doi |
https://doi.org/10.4103/1995-7645.231469 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
4 |
container_start_page |
285 |
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1766343106453569536 |