NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system.
BACKGROUND:Myriad infectious and noninfectious causes of encephalomyelitis (EM) have similar clinical manifestations, presenting serious challenges to diagnosis and treatment. Metabolomics of cerebrospinal fluid (CSF) was explored as a method of differentiating among neurological diseases causing EM...
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ftdoajarticles:oai:doaj.org/article:3aa1082067824612992ccdc101c68946 2023-05-15T15:03:04+02:00 NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. Caitlin D French Rodney E Willoughby Amy Pan Susan J Wong John F Foley L Joseph Wheat Josefina Fernandez Rafael Encarnacion Joanne M Ondrush Naaz Fatteh Andres Paez Dan David Waleed Javaid Ioana G Amzuta Anne M Neilan Gregory K Robbins Andrew M Brunner William T Hu Darya O Mishchuk Carolyn M Slupsky 2018-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007045 https://doaj.org/article/3aa1082067824612992ccdc101c68946 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007045 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007045 https://doaj.org/article/3aa1082067824612992ccdc101c68946 PLoS Neglected Tropical Diseases, Vol 12, Iss 12, p e0007045 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007045 2022-12-31T13:48:05Z BACKGROUND:Myriad infectious and noninfectious causes of encephalomyelitis (EM) have similar clinical manifestations, presenting serious challenges to diagnosis and treatment. Metabolomics of cerebrospinal fluid (CSF) was explored as a method of differentiating among neurological diseases causing EM using a single CSF sample. METHODOLOGY/PRINCIPAL FINDINGS:1H NMR metabolomics was applied to CSF samples from 27 patients with a laboratory-confirmed disease, including Lyme disease or West Nile Virus meningoencephalitis, multiple sclerosis, rabies, or Histoplasma meningitis, and 25 controls. Cluster analyses distinguished samples by infection status and moderately by pathogen, with shared and differentiating metabolite patterns observed among diseases. CART analysis predicted infection status with 100% sensitivity and 93% specificity. CONCLUSIONS/SIGNIFICANCE:These preliminary results suggest the potential utility of CSF metabolomics as a rapid screening test to enhance diagnostic accuracies and improve patient outcomes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 12 e0007045 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Caitlin D French Rodney E Willoughby Amy Pan Susan J Wong John F Foley L Joseph Wheat Josefina Fernandez Rafael Encarnacion Joanne M Ondrush Naaz Fatteh Andres Paez Dan David Waleed Javaid Ioana G Amzuta Anne M Neilan Gregory K Robbins Andrew M Brunner William T Hu Darya O Mishchuk Carolyn M Slupsky NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Myriad infectious and noninfectious causes of encephalomyelitis (EM) have similar clinical manifestations, presenting serious challenges to diagnosis and treatment. Metabolomics of cerebrospinal fluid (CSF) was explored as a method of differentiating among neurological diseases causing EM using a single CSF sample. METHODOLOGY/PRINCIPAL FINDINGS:1H NMR metabolomics was applied to CSF samples from 27 patients with a laboratory-confirmed disease, including Lyme disease or West Nile Virus meningoencephalitis, multiple sclerosis, rabies, or Histoplasma meningitis, and 25 controls. Cluster analyses distinguished samples by infection status and moderately by pathogen, with shared and differentiating metabolite patterns observed among diseases. CART analysis predicted infection status with 100% sensitivity and 93% specificity. CONCLUSIONS/SIGNIFICANCE:These preliminary results suggest the potential utility of CSF metabolomics as a rapid screening test to enhance diagnostic accuracies and improve patient outcomes. |
format |
Article in Journal/Newspaper |
author |
Caitlin D French Rodney E Willoughby Amy Pan Susan J Wong John F Foley L Joseph Wheat Josefina Fernandez Rafael Encarnacion Joanne M Ondrush Naaz Fatteh Andres Paez Dan David Waleed Javaid Ioana G Amzuta Anne M Neilan Gregory K Robbins Andrew M Brunner William T Hu Darya O Mishchuk Carolyn M Slupsky |
author_facet |
Caitlin D French Rodney E Willoughby Amy Pan Susan J Wong John F Foley L Joseph Wheat Josefina Fernandez Rafael Encarnacion Joanne M Ondrush Naaz Fatteh Andres Paez Dan David Waleed Javaid Ioana G Amzuta Anne M Neilan Gregory K Robbins Andrew M Brunner William T Hu Darya O Mishchuk Carolyn M Slupsky |
author_sort |
Caitlin D French |
title |
NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
title_short |
NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
title_full |
NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
title_fullStr |
NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
title_full_unstemmed |
NMR metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
title_sort |
nmr metabolomics of cerebrospinal fluid differentiates inflammatory diseases of the central nervous system. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0007045 https://doaj.org/article/3aa1082067824612992ccdc101c68946 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 12, p e0007045 (2018) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007045 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007045 https://doaj.org/article/3aa1082067824612992ccdc101c68946 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007045 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
12 |
container_start_page |
e0007045 |
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1766334981718671360 |