Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein.
Dengue virus (DENV) is the most prevalent and burdensome arbovirus transmitted by Aedes mosquitoes, against which there is only a limited licensed vaccine and no approved drug treatment. A Chromobacterium species, C. sp. Panama, isolated from the midgut of A. aegypti is able to inhibit DENV replicat...
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ftdoajarticles:oai:doaj.org/article:3a8bf3ef73d846a38401043248f3175c 2023-05-15T15:06:08+02:00 Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. Raúl G Saraiva Jingru Fang Seokyoung Kang Yesseinia I Angleró-Rodríguez Yuemei Dong George Dimopoulos 2018-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006443 https://doaj.org/article/3a8bf3ef73d846a38401043248f3175c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5937796?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006443 https://doaj.org/article/3a8bf3ef73d846a38401043248f3175c PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006443 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006443 2022-12-31T03:27:49Z Dengue virus (DENV) is the most prevalent and burdensome arbovirus transmitted by Aedes mosquitoes, against which there is only a limited licensed vaccine and no approved drug treatment. A Chromobacterium species, C. sp. Panama, isolated from the midgut of A. aegypti is able to inhibit DENV replication within the mosquito and in vitro. Here we show that C. sp. Panama mediates its anti-DENV activity through secreted factors that are proteinous in nature. The inhibitory effect occurs prior to virus attachment to cells, and is attributed to a factor that destabilizes the virion by promoting the degradation of the viral envelope protein. Bioassay-guided fractionation, coupled with mass spectrometry, allowed for the identification of a C. sp. Panama-secreted neutral protease and an aminopeptidase that are co-expressed and appear to act synergistically to degrade the viral envelope (E) protein and thus prevent viral attachment and subsequent infection of cells. This is the first study characterizing the anti-DENV activity of a common soil and mosquito-associated bacterium, thereby contributing towards understanding how such bacteria may limit disease transmission, and providing new tools for dengue prevention and therapeutics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 4 e0006443 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Raúl G Saraiva Jingru Fang Seokyoung Kang Yesseinia I Angleró-Rodríguez Yuemei Dong George Dimopoulos Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Dengue virus (DENV) is the most prevalent and burdensome arbovirus transmitted by Aedes mosquitoes, against which there is only a limited licensed vaccine and no approved drug treatment. A Chromobacterium species, C. sp. Panama, isolated from the midgut of A. aegypti is able to inhibit DENV replication within the mosquito and in vitro. Here we show that C. sp. Panama mediates its anti-DENV activity through secreted factors that are proteinous in nature. The inhibitory effect occurs prior to virus attachment to cells, and is attributed to a factor that destabilizes the virion by promoting the degradation of the viral envelope protein. Bioassay-guided fractionation, coupled with mass spectrometry, allowed for the identification of a C. sp. Panama-secreted neutral protease and an aminopeptidase that are co-expressed and appear to act synergistically to degrade the viral envelope (E) protein and thus prevent viral attachment and subsequent infection of cells. This is the first study characterizing the anti-DENV activity of a common soil and mosquito-associated bacterium, thereby contributing towards understanding how such bacteria may limit disease transmission, and providing new tools for dengue prevention and therapeutics. |
format |
Article in Journal/Newspaper |
author |
Raúl G Saraiva Jingru Fang Seokyoung Kang Yesseinia I Angleró-Rodríguez Yuemei Dong George Dimopoulos |
author_facet |
Raúl G Saraiva Jingru Fang Seokyoung Kang Yesseinia I Angleró-Rodríguez Yuemei Dong George Dimopoulos |
author_sort |
Raúl G Saraiva |
title |
Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. |
title_short |
Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. |
title_full |
Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. |
title_fullStr |
Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. |
title_full_unstemmed |
Aminopeptidase secreted by Chromobacterium sp. Panama inhibits dengue virus infection by degrading the E protein. |
title_sort |
aminopeptidase secreted by chromobacterium sp. panama inhibits dengue virus infection by degrading the e protein. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006443 https://doaj.org/article/3a8bf3ef73d846a38401043248f3175c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006443 (2018) |
op_relation |
http://europepmc.org/articles/PMC5937796?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006443 https://doaj.org/article/3a8bf3ef73d846a38401043248f3175c |
op_doi |
https://doi.org/10.1371/journal.pntd.0006443 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
4 |
container_start_page |
e0006443 |
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1766337788366553088 |