Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode.
BACKGROUND: Fatty acid (FA) binding proteins (FABPs) of helminths are implicated in acquisition and utilization of host-derived hydrophobic substances, as well as in signaling and cellular interactions. We previously demonstrated that secretory hydrophobic ligand binding proteins (HLBPs) of Taenia s...
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ftdoajarticles:oai:doaj.org/article:3a7d9c9463374e64b2052f20f4bbd159 2023-05-15T15:14:54+02:00 Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. Seon-Hee Kim Young-An Bae Hyun-Jong Yang Joo-Ho Shin Sylvia Paz Diaz-Camacho Yukifumi Nawa Insug Kang Yoon Kong 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001868 https://doaj.org/article/3a7d9c9463374e64b2052f20f4bbd159 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3493614?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001868 https://doaj.org/article/3a7d9c9463374e64b2052f20f4bbd159 PLoS Neglected Tropical Diseases, Vol 6, Iss 10, p e1868 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001868 2022-12-31T13:52:54Z BACKGROUND: Fatty acid (FA) binding proteins (FABPs) of helminths are implicated in acquisition and utilization of host-derived hydrophobic substances, as well as in signaling and cellular interactions. We previously demonstrated that secretory hydrophobic ligand binding proteins (HLBPs) of Taenia solium metacestode (TsM), a causative agent of neurocysticercosis (NC), shuttle FAs in the surrounding host tissues and inwardly transport the FAs across the parasite syncytial membrane. However, the protein molecules responsible for the intracellular trafficking and assimilation of FAs have remained elusive. METHODOLOGY/PRINCIPAL FINDINGS: We isolated two novel TsMFABP genes (TsMFABP1 and TsMFABP2), which encoded 133- and 136-amino acid polypeptides with predicted molecular masses of 14.3 and 14.8 kDa, respectively. They shared 45% sequence identity with each other and 15-95% with other related-members. Homology modeling demonstrated a characteristic β-barrel composed of 10 anti-parallel β-strands and two α-helices. TsMFABP2 harbored two additional loops between β-strands two and three, and β-strands six and seven, respectively. TsMFABP1 was secreted into cyst fluid and surrounding environments, whereas TsMFABP2 was intracellularly confined. Partially purified native proteins migrated to 15 kDa with different isoelectric points of 9.2 (TsMFABP1) and 8.4 (TsMFABP2). Both native and recombinant proteins bound to 11-([5-dimethylaminonaphthalene-1-sulfonyl]amino)undecannoic acid, dansyl-DL-α-amino-caprylic acid, cis-parinaric acid and retinol, which were competitively inhibited by oleic acid. TsMFABP1 exhibited high affinity toward FA analogs. TsMFABPs showed weak binding activity to retinol, but TsMFABP2 showed relatively high affinity. Isolation of two distinct genes from an individual genome strongly suggested their paralogous nature. Abundant expression of TsMFABP1 and TsMFABP2 in the canal region of worm matched well with the histological distributions of lipids and retinol. CONCLUSIONS/SIGNIFICANCE: The divergent ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 10 e1868 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Seon-Hee Kim Young-An Bae Hyun-Jong Yang Joo-Ho Shin Sylvia Paz Diaz-Camacho Yukifumi Nawa Insug Kang Yoon Kong Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Fatty acid (FA) binding proteins (FABPs) of helminths are implicated in acquisition and utilization of host-derived hydrophobic substances, as well as in signaling and cellular interactions. We previously demonstrated that secretory hydrophobic ligand binding proteins (HLBPs) of Taenia solium metacestode (TsM), a causative agent of neurocysticercosis (NC), shuttle FAs in the surrounding host tissues and inwardly transport the FAs across the parasite syncytial membrane. However, the protein molecules responsible for the intracellular trafficking and assimilation of FAs have remained elusive. METHODOLOGY/PRINCIPAL FINDINGS: We isolated two novel TsMFABP genes (TsMFABP1 and TsMFABP2), which encoded 133- and 136-amino acid polypeptides with predicted molecular masses of 14.3 and 14.8 kDa, respectively. They shared 45% sequence identity with each other and 15-95% with other related-members. Homology modeling demonstrated a characteristic β-barrel composed of 10 anti-parallel β-strands and two α-helices. TsMFABP2 harbored two additional loops between β-strands two and three, and β-strands six and seven, respectively. TsMFABP1 was secreted into cyst fluid and surrounding environments, whereas TsMFABP2 was intracellularly confined. Partially purified native proteins migrated to 15 kDa with different isoelectric points of 9.2 (TsMFABP1) and 8.4 (TsMFABP2). Both native and recombinant proteins bound to 11-([5-dimethylaminonaphthalene-1-sulfonyl]amino)undecannoic acid, dansyl-DL-α-amino-caprylic acid, cis-parinaric acid and retinol, which were competitively inhibited by oleic acid. TsMFABP1 exhibited high affinity toward FA analogs. TsMFABPs showed weak binding activity to retinol, but TsMFABP2 showed relatively high affinity. Isolation of two distinct genes from an individual genome strongly suggested their paralogous nature. Abundant expression of TsMFABP1 and TsMFABP2 in the canal region of worm matched well with the histological distributions of lipids and retinol. CONCLUSIONS/SIGNIFICANCE: The divergent ... |
format |
Article in Journal/Newspaper |
author |
Seon-Hee Kim Young-An Bae Hyun-Jong Yang Joo-Ho Shin Sylvia Paz Diaz-Camacho Yukifumi Nawa Insug Kang Yoon Kong |
author_facet |
Seon-Hee Kim Young-An Bae Hyun-Jong Yang Joo-Ho Shin Sylvia Paz Diaz-Camacho Yukifumi Nawa Insug Kang Yoon Kong |
author_sort |
Seon-Hee Kim |
title |
Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. |
title_short |
Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. |
title_full |
Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. |
title_fullStr |
Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. |
title_full_unstemmed |
Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode. |
title_sort |
structural and binding properties of two paralogous fatty acid binding proteins of taenia solium metacestode. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001868 https://doaj.org/article/3a7d9c9463374e64b2052f20f4bbd159 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 10, p e1868 (2012) |
op_relation |
http://europepmc.org/articles/PMC3493614?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001868 https://doaj.org/article/3a7d9c9463374e64b2052f20f4bbd159 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001868 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
10 |
container_start_page |
e1868 |
_version_ |
1766345302785130496 |