Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan.
Background Amebiasis, caused by Entamoeba histolytica, is spreading in developing countries and in many developed countries as a sexually transmitted infection. Here, we evaluated the efficacy of serological screening to identify asymptomatic E. histolytica infection as a potential epidemiological c...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:3a7200b10a8841c8a89f804f5e2ad6ee 2023-05-15T15:08:20+02:00 Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. Yasuaki Yanagawa Rieko Shimogawara Misao Takano Takahiro Aoki Daisuke Mizushima Hiroyuki Gatanaga Yoshimi Kikuchi Shinichi Oka Kenji Yagita Koji Watanabe 2022-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009793 https://doaj.org/article/3a7200b10a8841c8a89f804f5e2ad6ee EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009793 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009793 https://doaj.org/article/3a7200b10a8841c8a89f804f5e2ad6ee PLoS Neglected Tropical Diseases, Vol 16, Iss 4, p e0009793 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009793 2022-12-31T02:25:30Z Background Amebiasis, caused by Entamoeba histolytica, is spreading in developing countries and in many developed countries as a sexually transmitted infection. Here, we evaluated the efficacy of serological screening to identify asymptomatic E. histolytica infection as a potential epidemiological control measure to limit its spread. Methodology/principal findings This cross-sectional study was carried out between January and March 2021 in an HIV-negative men who have sex with men (MSM) cohort at the National Center for Global Health and Medicine. Serological screening was performed using a commercially available ELISA kit. For seropositive individuals, we performed stool polymerase chain reaction (PCR) to determine current E. histolytica infection. We performed E. histolytica serological screening of 312 participants. None had a history of E. histolytica infection prior to the study. The overall E. histolytica seropositivity was 6.7% (21/312), which was similar to that found by the rapid plasma reagin test (17/312). We identified current infection in 8 of 20 seropositive participants (40.0%) by stool PCR. Conclusions/significance Our serological screening approach constitutes a potentially practical epidemiological strategy. Active epidemiological surveys, in combination with an effective screening strategy for asymptomatically infected individuals, should be applied to help reduce sexually transmitted E. histolytica infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 4 e0009793 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yasuaki Yanagawa Rieko Shimogawara Misao Takano Takahiro Aoki Daisuke Mizushima Hiroyuki Gatanaga Yoshimi Kikuchi Shinichi Oka Kenji Yagita Koji Watanabe Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Amebiasis, caused by Entamoeba histolytica, is spreading in developing countries and in many developed countries as a sexually transmitted infection. Here, we evaluated the efficacy of serological screening to identify asymptomatic E. histolytica infection as a potential epidemiological control measure to limit its spread. Methodology/principal findings This cross-sectional study was carried out between January and March 2021 in an HIV-negative men who have sex with men (MSM) cohort at the National Center for Global Health and Medicine. Serological screening was performed using a commercially available ELISA kit. For seropositive individuals, we performed stool polymerase chain reaction (PCR) to determine current E. histolytica infection. We performed E. histolytica serological screening of 312 participants. None had a history of E. histolytica infection prior to the study. The overall E. histolytica seropositivity was 6.7% (21/312), which was similar to that found by the rapid plasma reagin test (17/312). We identified current infection in 8 of 20 seropositive participants (40.0%) by stool PCR. Conclusions/significance Our serological screening approach constitutes a potentially practical epidemiological strategy. Active epidemiological surveys, in combination with an effective screening strategy for asymptomatically infected individuals, should be applied to help reduce sexually transmitted E. histolytica infections. |
format |
Article in Journal/Newspaper |
author |
Yasuaki Yanagawa Rieko Shimogawara Misao Takano Takahiro Aoki Daisuke Mizushima Hiroyuki Gatanaga Yoshimi Kikuchi Shinichi Oka Kenji Yagita Koji Watanabe |
author_facet |
Yasuaki Yanagawa Rieko Shimogawara Misao Takano Takahiro Aoki Daisuke Mizushima Hiroyuki Gatanaga Yoshimi Kikuchi Shinichi Oka Kenji Yagita Koji Watanabe |
author_sort |
Yasuaki Yanagawa |
title |
Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. |
title_short |
Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. |
title_full |
Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. |
title_fullStr |
Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. |
title_full_unstemmed |
Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan. |
title_sort |
identification of asymptomatic entamoeba histolytica infection by a serological screening test: a cross-sectional study of an hiv-negative men who have sex with men cohort in japan. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0009793 https://doaj.org/article/3a7200b10a8841c8a89f804f5e2ad6ee |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 4, p e0009793 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009793 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009793 https://doaj.org/article/3a7200b10a8841c8a89f804f5e2ad6ee |
op_doi |
https://doi.org/10.1371/journal.pntd.0009793 |
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PLOS Neglected Tropical Diseases |
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16 |
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4 |
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e0009793 |
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