Analgesic and side effects of intravenous recombinant Phα1β
ABSTRACT Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clin...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2019-0070 https://doaj.org/article/3a6a31d0259a4d89a0d327cf5d52aa15 |
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ftdoajarticles:oai:doaj.org/article:3a6a31d0259a4d89a0d327cf5d52aa15 2023-05-15T15:12:56+02:00 Analgesic and side effects of intravenous recombinant Phα1β Flavia Karine Rigo Mateus Fortes Rossato Vanessa Borges Juliana Figueira da Silva Elizete Maria Rita Pereira Ricardo Andrez Machado de Ávila Gabriela Trevisan Duana Carvalho dos Santos Danuza Montijo Diniz Marco Aurélio Romano Silva Célio José de Castro Junior Thiago Mattar Cunha Juliano Ferreira Marcus Vinicius Gomez 2020-04-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2019-0070 https://doaj.org/article/3a6a31d0259a4d89a0d327cf5d52aa15 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100309&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190070.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0070 https://doaj.org/article/3a6a31d0259a4d89a0d327cf5d52aa15 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) Recombinant Phα1β Analgesia Neuropathic pain Intravenous drug delivery system Side effects Cardiac function Motor activity Biochemicals Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0070 2022-12-31T14:36:06Z ABSTRACT Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1β using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1β toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1β may be feasible for drug-induced analgesia, without causing any severe side effects. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Recombinant Phα1β Analgesia Neuropathic pain Intravenous drug delivery system Side effects Cardiac function Motor activity Biochemicals Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Recombinant Phα1β Analgesia Neuropathic pain Intravenous drug delivery system Side effects Cardiac function Motor activity Biochemicals Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Flavia Karine Rigo Mateus Fortes Rossato Vanessa Borges Juliana Figueira da Silva Elizete Maria Rita Pereira Ricardo Andrez Machado de Ávila Gabriela Trevisan Duana Carvalho dos Santos Danuza Montijo Diniz Marco Aurélio Romano Silva Célio José de Castro Junior Thiago Mattar Cunha Juliano Ferreira Marcus Vinicius Gomez Analgesic and side effects of intravenous recombinant Phα1β |
topic_facet |
Recombinant Phα1β Analgesia Neuropathic pain Intravenous drug delivery system Side effects Cardiac function Motor activity Biochemicals Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
ABSTRACT Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1β using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1β toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1β may be feasible for drug-induced analgesia, without causing any severe side effects. |
format |
Article in Journal/Newspaper |
author |
Flavia Karine Rigo Mateus Fortes Rossato Vanessa Borges Juliana Figueira da Silva Elizete Maria Rita Pereira Ricardo Andrez Machado de Ávila Gabriela Trevisan Duana Carvalho dos Santos Danuza Montijo Diniz Marco Aurélio Romano Silva Célio José de Castro Junior Thiago Mattar Cunha Juliano Ferreira Marcus Vinicius Gomez |
author_facet |
Flavia Karine Rigo Mateus Fortes Rossato Vanessa Borges Juliana Figueira da Silva Elizete Maria Rita Pereira Ricardo Andrez Machado de Ávila Gabriela Trevisan Duana Carvalho dos Santos Danuza Montijo Diniz Marco Aurélio Romano Silva Célio José de Castro Junior Thiago Mattar Cunha Juliano Ferreira Marcus Vinicius Gomez |
author_sort |
Flavia Karine Rigo |
title |
Analgesic and side effects of intravenous recombinant Phα1β |
title_short |
Analgesic and side effects of intravenous recombinant Phα1β |
title_full |
Analgesic and side effects of intravenous recombinant Phα1β |
title_fullStr |
Analgesic and side effects of intravenous recombinant Phα1β |
title_full_unstemmed |
Analgesic and side effects of intravenous recombinant Phα1β |
title_sort |
analgesic and side effects of intravenous recombinant phα1β |
publisher |
SciELO |
publishDate |
2020 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0070 https://doaj.org/article/3a6a31d0259a4d89a0d327cf5d52aa15 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100309&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190070.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0070 https://doaj.org/article/3a6a31d0259a4d89a0d327cf5d52aa15 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0070 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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