Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma
Introduction: Polychlorinated biphenyls are among the five most toxic persistent contaminants for living organisms according to the Agency for Toxic Substances and Disease Registry (ATSDR). Objective: To standardize and validate an analytical method to determine and quantify polychlorinated biphenyl...
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ftdoajarticles:oai:doaj.org/article:3a4bff7d55b14ddcba213d1099570d41 2023-05-15T15:09:20+02:00 Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma Boris Santiago Ávila Carolina Ramírez 2017-12-01T00:00:00Z https://doi.org/10.7705/biomedica.v37i4.3529 https://doaj.org/article/3a4bff7d55b14ddcba213d1099570d41 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3529 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i4.3529 https://doaj.org/article/3a4bff7d55b14ddcba213d1099570d41 Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 4, Pp 561-570 (2017) Polychlorinated biphenyls blood plasma chromatography gas mass spectrometry chemistry analytical Medicine R Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.7705/biomedica.v37i4.3529 2022-12-31T00:56:15Z Introduction: Polychlorinated biphenyls are among the five most toxic persistent contaminants for living organisms according to the Agency for Toxic Substances and Disease Registry (ATSDR). Objective: To standardize and validate an analytical method to determine and quantify polychlorinated biphenyl indicators in samples from blood plasma by means of gas chromatography-mass spectrometry. Materials and methods: We fortified a plasma pool to do the matrix assays. Additionally, we used the NIST SRM® 1958 reference material for the veracity and intermediate accuracy assays. Results: Methodology recovery percentages ranged between 88.4 and 97.5%, and the bias was less than 20%. Detection and quantification limits were 0.04 μg/L and 0.10 μg/L, respectively, for all polychlorinated biphenyl indicators. The linearity represented by the determination coefficient (R2) varied between 0.9866 and 0.9886. Accuracy, expressed as relative standard deviation was less than 20% in all the linear work range (0.5-500 μg/L). Finally, we analyzed 115 samples from Colombian population in various zones of the country and we found 65 positive samples, from which two samples were above HBM-II (7.0 μg/L, 2XΣPCB 138, 153, 180), and two, above HBM-I (3.5 μg/L, 2XΣPCB 138, 153, 180). Conclusion: The method we developed is accurate for PCB analysis in blood plasma samples and could be used for biological surveillance of these contaminants in the Colombian population Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 37 4 561 |
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Polychlorinated biphenyls blood plasma chromatography gas mass spectrometry chemistry analytical Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Polychlorinated biphenyls blood plasma chromatography gas mass spectrometry chemistry analytical Medicine R Arctic medicine. Tropical medicine RC955-962 Boris Santiago Ávila Carolina Ramírez Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
topic_facet |
Polychlorinated biphenyls blood plasma chromatography gas mass spectrometry chemistry analytical Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Polychlorinated biphenyls are among the five most toxic persistent contaminants for living organisms according to the Agency for Toxic Substances and Disease Registry (ATSDR). Objective: To standardize and validate an analytical method to determine and quantify polychlorinated biphenyl indicators in samples from blood plasma by means of gas chromatography-mass spectrometry. Materials and methods: We fortified a plasma pool to do the matrix assays. Additionally, we used the NIST SRM® 1958 reference material for the veracity and intermediate accuracy assays. Results: Methodology recovery percentages ranged between 88.4 and 97.5%, and the bias was less than 20%. Detection and quantification limits were 0.04 μg/L and 0.10 μg/L, respectively, for all polychlorinated biphenyl indicators. The linearity represented by the determination coefficient (R2) varied between 0.9866 and 0.9886. Accuracy, expressed as relative standard deviation was less than 20% in all the linear work range (0.5-500 μg/L). Finally, we analyzed 115 samples from Colombian population in various zones of the country and we found 65 positive samples, from which two samples were above HBM-II (7.0 μg/L, 2XΣPCB 138, 153, 180), and two, above HBM-I (3.5 μg/L, 2XΣPCB 138, 153, 180). Conclusion: The method we developed is accurate for PCB analysis in blood plasma samples and could be used for biological surveillance of these contaminants in the Colombian population |
format |
Article in Journal/Newspaper |
author |
Boris Santiago Ávila Carolina Ramírez |
author_facet |
Boris Santiago Ávila Carolina Ramírez |
author_sort |
Boris Santiago Ávila |
title |
Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
title_short |
Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
title_full |
Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
title_fullStr |
Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
title_full_unstemmed |
Validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
title_sort |
validation of an analytical methodology to determine polychlorinated biphenyls in samples from blood plasma |
publisher |
Instituto Nacional de Salud |
publishDate |
2017 |
url |
https://doi.org/10.7705/biomedica.v37i4.3529 https://doaj.org/article/3a4bff7d55b14ddcba213d1099570d41 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 4, Pp 561-570 (2017) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3529 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i4.3529 https://doaj.org/article/3a4bff7d55b14ddcba213d1099570d41 |
op_doi |
https://doi.org/10.7705/biomedica.v37i4.3529 |
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Biomédica |
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37 |
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