The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one
Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). Female eels need to ovulate within 18 h after injection to release good quality eggs. Progesterone (P), as an upstream precursor of DHP, may promote endogenous DHP p...
Published in: | Frontiers in Physiology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fphys.2023.1207542 https://doaj.org/article/3a41b27c42ee4d098533bd4f7d195d1f |
id |
ftdoajarticles:oai:doaj.org/article:3a41b27c42ee4d098533bd4f7d195d1f |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3a41b27c42ee4d098533bd4f7d195d1f 2023-09-05T13:12:01+02:00 The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one Pauline Jéhannet Arjan P. Palstra Miriam Meijerhof Henk Schipper Ignacio Nebot Giménez Ron P. Dirks William Swinkels Leon T. N. Heinsbroek Hans Komen 2023-08-01T00:00:00Z https://doi.org/10.3389/fphys.2023.1207542 https://doaj.org/article/3a41b27c42ee4d098533bd4f7d195d1f EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2023.1207542/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2023.1207542 https://doaj.org/article/3a41b27c42ee4d098533bd4f7d195d1f Frontiers in Physiology, Vol 14 (2023) aquaculture eel reproduction spawning induction maturation-inducing hormone RNA-seq Physiology QP1-981 article 2023 ftdoajarticles https://doi.org/10.3389/fphys.2023.1207542 2023-08-13T00:39:44Z Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). Female eels need to ovulate within 18 h after injection to release good quality eggs. Progesterone (P), as an upstream precursor of DHP, may promote endogenous DHP production and improve egg quality. The purpose of this study was therefore to compare treatment of P with DHP on batch level, in vitro, to determine dose-response effects, and in vivo, at a single dose. For the in vitro experiment, ovarian tissue was extracted and placed in culture plates containing hormone-free medium and media supplemented with the treatment: DHP at 1, 10 and 100 ng mL−1, or P at 10, 100 and 1,000 ng mL−1. At the start of incubation, the folliculated oocytes were sampled for histology, microscopy and qPCR. After incubation for 12 and 18 h, the oocytes were sampled for microscopy and qPCR analysis. For the in vivo experiment, females were either injected with DHP or P at a dose of 2 mg kg−1 to assess their effects on ovulation and reproductive success. At the moment of release, eggs were sampled for RNA sequencing to compare effects of DHP and P on the expression of genes involved in egg quality aspects. Remaining eggs were fertilized and larval viability was recorded. Both DHP and P were able to induce GVBD (DHP at 10 and 100 ng mL−1, P at 100 and 1,000 ng mL−1) in vitro. Expression of genes involved in oocyte maturation and ovulation was similar in vitro for both DHP and P treatments. Regarding the in vivo results, RNAseq results reflected similar DHP and P effects on the expression of genes involved in egg quality aspects. Females injected with either DHP or P ovulated, released eggs, and were equally able to produce larvae without any differences in reproductive success. Our results support the conclusion that DHP and P work equally well in vitro and in vivo. P is more attractive to apply as the price is 3,000 times lower than the price of DHP. Article in Journal/Newspaper Anguilla anguilla Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 14 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
aquaculture eel reproduction spawning induction maturation-inducing hormone RNA-seq Physiology QP1-981 |
spellingShingle |
aquaculture eel reproduction spawning induction maturation-inducing hormone RNA-seq Physiology QP1-981 Pauline Jéhannet Arjan P. Palstra Miriam Meijerhof Henk Schipper Ignacio Nebot Giménez Ron P. Dirks William Swinkels Leon T. N. Heinsbroek Hans Komen The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
topic_facet |
aquaculture eel reproduction spawning induction maturation-inducing hormone RNA-seq Physiology QP1-981 |
description |
Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). Female eels need to ovulate within 18 h after injection to release good quality eggs. Progesterone (P), as an upstream precursor of DHP, may promote endogenous DHP production and improve egg quality. The purpose of this study was therefore to compare treatment of P with DHP on batch level, in vitro, to determine dose-response effects, and in vivo, at a single dose. For the in vitro experiment, ovarian tissue was extracted and placed in culture plates containing hormone-free medium and media supplemented with the treatment: DHP at 1, 10 and 100 ng mL−1, or P at 10, 100 and 1,000 ng mL−1. At the start of incubation, the folliculated oocytes were sampled for histology, microscopy and qPCR. After incubation for 12 and 18 h, the oocytes were sampled for microscopy and qPCR analysis. For the in vivo experiment, females were either injected with DHP or P at a dose of 2 mg kg−1 to assess their effects on ovulation and reproductive success. At the moment of release, eggs were sampled for RNA sequencing to compare effects of DHP and P on the expression of genes involved in egg quality aspects. Remaining eggs were fertilized and larval viability was recorded. Both DHP and P were able to induce GVBD (DHP at 10 and 100 ng mL−1, P at 100 and 1,000 ng mL−1) in vitro. Expression of genes involved in oocyte maturation and ovulation was similar in vitro for both DHP and P treatments. Regarding the in vivo results, RNAseq results reflected similar DHP and P effects on the expression of genes involved in egg quality aspects. Females injected with either DHP or P ovulated, released eggs, and were equally able to produce larvae without any differences in reproductive success. Our results support the conclusion that DHP and P work equally well in vitro and in vivo. P is more attractive to apply as the price is 3,000 times lower than the price of DHP. |
format |
Article in Journal/Newspaper |
author |
Pauline Jéhannet Arjan P. Palstra Miriam Meijerhof Henk Schipper Ignacio Nebot Giménez Ron P. Dirks William Swinkels Leon T. N. Heinsbroek Hans Komen |
author_facet |
Pauline Jéhannet Arjan P. Palstra Miriam Meijerhof Henk Schipper Ignacio Nebot Giménez Ron P. Dirks William Swinkels Leon T. N. Heinsbroek Hans Komen |
author_sort |
Pauline Jéhannet |
title |
The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
title_short |
The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
title_full |
The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
title_fullStr |
The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
title_full_unstemmed |
The induction of oocyte maturation and ovulation in the European eel (Anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
title_sort |
induction of oocyte maturation and ovulation in the european eel (anguilla anguilla): in vitro and in vivo comparison of progesterone with 17α,20β-dihydroxy-4-pregnen-3-one |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fphys.2023.1207542 https://doaj.org/article/3a41b27c42ee4d098533bd4f7d195d1f |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Frontiers in Physiology, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2023.1207542/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2023.1207542 https://doaj.org/article/3a41b27c42ee4d098533bd4f7d195d1f |
op_doi |
https://doi.org/10.3389/fphys.2023.1207542 |
container_title |
Frontiers in Physiology |
container_volume |
14 |
_version_ |
1776198549886730240 |