Insecticide resistance status in Anopheles gambiae in southern Benin
Abstract Background The emergence of pyrethroid resistance in Anopheles gambiae has become a serious concern to the future success of malaria control. In Benin, the National Malaria Control Programme has recently planned to scaling up long-lasting insecticidal nets (LLINs) and indoor residual sprayi...
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ftdoajarticles:oai:doaj.org/article:3a3f12b868624ddea8a36b4a89a170b7 2023-05-15T15:16:01+02:00 Insecticide resistance status in Anopheles gambiae in southern Benin Corbel Vincent Bio-Banganna Sahabi Moiroux Nicolas Asidi Alex Padonou Gil Yadouleton Anges W N'guessan Raphael Gbenou Dina Yacoubou Imorou Gazard Kinde Akogbeto Martin C 2010-03-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-83 https://doaj.org/article/3a3f12b868624ddea8a36b4a89a170b7 EN eng BMC http://www.malariajournal.com/content/9/1/83 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-83 1475-2875 https://doaj.org/article/3a3f12b868624ddea8a36b4a89a170b7 Malaria Journal, Vol 9, Iss 1, p 83 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-83 2022-12-31T09:15:40Z Abstract Background The emergence of pyrethroid resistance in Anopheles gambiae has become a serious concern to the future success of malaria control. In Benin, the National Malaria Control Programme has recently planned to scaling up long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) for malaria prevention. It is, therefore, crucial to monitor the level and type of insecticide resistance in An. gambiae , particularly in southern Benin where reduced efficacy of insecticide-treated nets (ITNs) and IRS has previously been reported. Methods The protocol was based on mosquito collection during both dry and rainy seasons across forty districts selected in southern Benin. Bioassay were performed on adults collected from the field to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, and bendiocarb 0.1%) following WHOPES guidelines. The species within An. gambiae complex, molecular form and presence of kdr and ace- 1 mutations were determined by PCR. Results Strong resistance to permethrin and DDT was found in An. gambiae populations from southern Benin, except in Aglangandan where mosquitoes were fully susceptible (mortality 100%) to all insecticides tested. PCR showed the presence of two sub-species of An. gambiae , namely An. gambiae s.s , and Anopheles melas , with a predominance for An. gambiae s.s (98%). The molecular M form of An. gambiae was predominant in southern Benin (97%). The kdr mutation was detected in all districts at various frequency (1% to 95%) whereas the Ace-1 mutation was found at a very low frequency (≤ 5%). Conclusion This study showed a widespread resistance to permethrin in An. gambiae populations from southern Benin, with a significant increase of kdr frequency compared to what was observed previously in Benin. The low frequency of Ace-1 recorded in all populations is encouraging for the use of bendiocarb as an alternative insecticide to pyrethroids for IRS in Benin. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Corbel Vincent Bio-Banganna Sahabi Moiroux Nicolas Asidi Alex Padonou Gil Yadouleton Anges W N'guessan Raphael Gbenou Dina Yacoubou Imorou Gazard Kinde Akogbeto Martin C Insecticide resistance status in Anopheles gambiae in southern Benin |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The emergence of pyrethroid resistance in Anopheles gambiae has become a serious concern to the future success of malaria control. In Benin, the National Malaria Control Programme has recently planned to scaling up long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) for malaria prevention. It is, therefore, crucial to monitor the level and type of insecticide resistance in An. gambiae , particularly in southern Benin where reduced efficacy of insecticide-treated nets (ITNs) and IRS has previously been reported. Methods The protocol was based on mosquito collection during both dry and rainy seasons across forty districts selected in southern Benin. Bioassay were performed on adults collected from the field to assess the susceptibility of malaria vectors to insecticide-impregnated papers (permethrin 0.75%, delthamethrin 0.05%, DDT 4%, and bendiocarb 0.1%) following WHOPES guidelines. The species within An. gambiae complex, molecular form and presence of kdr and ace- 1 mutations were determined by PCR. Results Strong resistance to permethrin and DDT was found in An. gambiae populations from southern Benin, except in Aglangandan where mosquitoes were fully susceptible (mortality 100%) to all insecticides tested. PCR showed the presence of two sub-species of An. gambiae , namely An. gambiae s.s , and Anopheles melas , with a predominance for An. gambiae s.s (98%). The molecular M form of An. gambiae was predominant in southern Benin (97%). The kdr mutation was detected in all districts at various frequency (1% to 95%) whereas the Ace-1 mutation was found at a very low frequency (≤ 5%). Conclusion This study showed a widespread resistance to permethrin in An. gambiae populations from southern Benin, with a significant increase of kdr frequency compared to what was observed previously in Benin. The low frequency of Ace-1 recorded in all populations is encouraging for the use of bendiocarb as an alternative insecticide to pyrethroids for IRS in Benin. |
format |
Article in Journal/Newspaper |
author |
Corbel Vincent Bio-Banganna Sahabi Moiroux Nicolas Asidi Alex Padonou Gil Yadouleton Anges W N'guessan Raphael Gbenou Dina Yacoubou Imorou Gazard Kinde Akogbeto Martin C |
author_facet |
Corbel Vincent Bio-Banganna Sahabi Moiroux Nicolas Asidi Alex Padonou Gil Yadouleton Anges W N'guessan Raphael Gbenou Dina Yacoubou Imorou Gazard Kinde Akogbeto Martin C |
author_sort |
Corbel Vincent |
title |
Insecticide resistance status in Anopheles gambiae in southern Benin |
title_short |
Insecticide resistance status in Anopheles gambiae in southern Benin |
title_full |
Insecticide resistance status in Anopheles gambiae in southern Benin |
title_fullStr |
Insecticide resistance status in Anopheles gambiae in southern Benin |
title_full_unstemmed |
Insecticide resistance status in Anopheles gambiae in southern Benin |
title_sort |
insecticide resistance status in anopheles gambiae in southern benin |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-83 https://doaj.org/article/3a3f12b868624ddea8a36b4a89a170b7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 83 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/83 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-83 1475-2875 https://doaj.org/article/3a3f12b868624ddea8a36b4a89a170b7 |
op_doi |
https://doi.org/10.1186/1475-2875-9-83 |
container_title |
Malaria Journal |
container_volume |
9 |
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1 |
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1766346342109544448 |