Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas
Diagnostic and parasite characterization and identification studies were carried out in human patients with cutaneous leishmaniasis lesions in Santiago del Estero, Northern Province of Argentina. Diagnostic procedures were biopsies of lesions for smears and inoculations in hamster, needle aspiration...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
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Online Access: | https://doi.org/10.1590/S0036-46651996000600005 https://doaj.org/article/3a1df23c17d9493d9175294c112752df |
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ftdoajarticles:oai:doaj.org/article:3a1df23c17d9493d9175294c112752df 2024-09-09T19:28:26+00:00 Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas C.A. Cuba Cuba C.O. Torno O. Ledesma E. Visciarelli S. Garcia M.I. Prat R. Costamagna L. Barbieri D.A. Evans 1996-12-01T00:00:00Z https://doi.org/10.1590/S0036-46651996000600005 https://doaj.org/article/3a1df23c17d9493d9175294c112752df EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000600005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000600005 0036-4665 1678-9946 https://doaj.org/article/3a1df23c17d9493d9175294c112752df Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 6, Pp 413-421 (1996) Cutaneous leishmaniasis Leishmania (Viannia) braziliensis Identification Santiago del Estero Argentina Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1996 ftdoajarticles https://doi.org/10.1590/S0036-46651996000600005 2024-08-05T17:49:30Z Diagnostic and parasite characterization and identification studies were carried out in human patients with cutaneous leishmaniasis lesions in Santiago del Estero, Northern Province of Argentina. Diagnostic procedures were biopsies of lesions for smears and inoculations in hamster, needle aspirations of material from ulcers for "in vitro" cultures. Immunodiagnostic techniques applied were IFAT-IgG and Montenegro skin test. Primary isolation of eight stocks of leishmanial parasites was achieved from patients with active lesions. All stocks were biologically characterized by their behaviour in hamster, measurements of amastigote and promastigotes and growth "in vitro". Eight stocks were characterized and identified at species level by their reactivity to a cross-panel of sub-genus and specie-specific Monoclonal Antibodies through an Indirect Immunofluorescence technique and a Dot-ELISA. We conclude from the serodeme analysis of Argentina stocks that: stocks MHOM/AR/92/SE-1; SE-2; SE-4; SE-8; SE-8-I; SE-30; SE-34 and SE-36 are Leishmania (Viannia) braziliensis. Three Leishmania stocks (SE-1; SE-2 and SE-30) did not react with one highly specie-specific Monoclonal Antibody (Clone: B-18, Leishmania (Viannia) braziliensis marker) disclosing two serodeme group patterns. Five out of eight soluble extracts of leishmanial promastigotes were electrophoresed on thin-layer starch gels and examined for the enzyme MPI, Mannose Phosphate Isomerase; MDH, Malate Dehydrogenase; 6PGD, 6 Phosphogluconate Dehydrogenase; NH, Nucleoside Hydrolase, 2-deoxyinosinc as substrate; SOD, Superoxide Dismutase; GPI, Glucose Phosphate Isomerase and ES, Esterase. From the isoenzyme studies we concluded that stocks: MHOM/AR/92/SE-1; SE-2; SE-4; SE-8 and SE-8-I are isoenzymatically Leishmania (Viannia) braziliensis. We need to analyze more enzymes before assigning them to a braziliensis zymodeme. Estudos de diagnóstico, caracterização parasitária e identificação foram conduzidos em pacientes humanos com lesões cutâneas de leishmaniose na Província ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Argentina Revista do Instituto de Medicina Tropical de São Paulo 38 6 413 421 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cutaneous leishmaniasis Leishmania (Viannia) braziliensis Identification Santiago del Estero Argentina Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Cutaneous leishmaniasis Leishmania (Viannia) braziliensis Identification Santiago del Estero Argentina Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 C.A. Cuba Cuba C.O. Torno O. Ledesma E. Visciarelli S. Garcia M.I. Prat R. Costamagna L. Barbieri D.A. Evans Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
topic_facet |
Cutaneous leishmaniasis Leishmania (Viannia) braziliensis Identification Santiago del Estero Argentina Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Diagnostic and parasite characterization and identification studies were carried out in human patients with cutaneous leishmaniasis lesions in Santiago del Estero, Northern Province of Argentina. Diagnostic procedures were biopsies of lesions for smears and inoculations in hamster, needle aspirations of material from ulcers for "in vitro" cultures. Immunodiagnostic techniques applied were IFAT-IgG and Montenegro skin test. Primary isolation of eight stocks of leishmanial parasites was achieved from patients with active lesions. All stocks were biologically characterized by their behaviour in hamster, measurements of amastigote and promastigotes and growth "in vitro". Eight stocks were characterized and identified at species level by their reactivity to a cross-panel of sub-genus and specie-specific Monoclonal Antibodies through an Indirect Immunofluorescence technique and a Dot-ELISA. We conclude from the serodeme analysis of Argentina stocks that: stocks MHOM/AR/92/SE-1; SE-2; SE-4; SE-8; SE-8-I; SE-30; SE-34 and SE-36 are Leishmania (Viannia) braziliensis. Three Leishmania stocks (SE-1; SE-2 and SE-30) did not react with one highly specie-specific Monoclonal Antibody (Clone: B-18, Leishmania (Viannia) braziliensis marker) disclosing two serodeme group patterns. Five out of eight soluble extracts of leishmanial promastigotes were electrophoresed on thin-layer starch gels and examined for the enzyme MPI, Mannose Phosphate Isomerase; MDH, Malate Dehydrogenase; 6PGD, 6 Phosphogluconate Dehydrogenase; NH, Nucleoside Hydrolase, 2-deoxyinosinc as substrate; SOD, Superoxide Dismutase; GPI, Glucose Phosphate Isomerase and ES, Esterase. From the isoenzyme studies we concluded that stocks: MHOM/AR/92/SE-1; SE-2; SE-4; SE-8 and SE-8-I are isoenzymatically Leishmania (Viannia) braziliensis. We need to analyze more enzymes before assigning them to a braziliensis zymodeme. Estudos de diagnóstico, caracterização parasitária e identificação foram conduzidos em pacientes humanos com lesões cutâneas de leishmaniose na Província ... |
format |
Article in Journal/Newspaper |
author |
C.A. Cuba Cuba C.O. Torno O. Ledesma E. Visciarelli S. Garcia M.I. Prat R. Costamagna L. Barbieri D.A. Evans |
author_facet |
C.A. Cuba Cuba C.O. Torno O. Ledesma E. Visciarelli S. Garcia M.I. Prat R. Costamagna L. Barbieri D.A. Evans |
author_sort |
C.A. Cuba Cuba |
title |
Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
title_short |
Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
title_full |
Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
title_fullStr |
Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
title_full_unstemmed |
Human cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in Santiago del Estero, Argentina: identification of parasites by monoclonal antibodies and isoenzymes Leishmaniose cutânea humana causada por Leishmania (Viannia) braziliensis na Província de Santiago del Estero, Argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
title_sort |
human cutaneous leishmaniasis caused by leishmania (viannia) braziliensis in santiago del estero, argentina: identification of parasites by monoclonal antibodies and isoenzymes leishmaniose cutânea humana causada por leishmania (viannia) braziliensis na província de santiago del estero, argentina: identificação dos parasitas por anticorpos monoclonais e isoenzimas |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1996 |
url |
https://doi.org/10.1590/S0036-46651996000600005 https://doaj.org/article/3a1df23c17d9493d9175294c112752df |
geographic |
Arctic Argentina |
geographic_facet |
Arctic Argentina |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 38, Iss 6, Pp 413-421 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651996000600005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651996000600005 0036-4665 1678-9946 https://doaj.org/article/3a1df23c17d9493d9175294c112752df |
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https://doi.org/10.1590/S0036-46651996000600005 |
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38 |
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413 |
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