Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR
Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. R...
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ftdoajarticles:oai:doaj.org/article:3a09a99de58d4b9dbb706920d15166ff 2023-05-15T15:00:11+02:00 Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR Maristela Oliveira Lara Thabata Coaglio Lucas Evanguedes kalapothakis Ronaldo Luis Thomasini Carla Jorge Machado https://doi.org/10.1590/0037-8682-0352-2017 https://doaj.org/article/3a09a99de58d4b9dbb706920d15166ff EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400528&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0352-2017 https://doaj.org/article/3a09a99de58d4b9dbb706920d15166ff Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 4, Pp 528-532 Polymerase chain reaction Bacterial DNA DNA extraction Nosocomial infection Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0352-2017 2022-12-30T21:39:30Z Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. RESULTS: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. CONCLUSIONS: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 51 4 528 532 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Polymerase chain reaction Bacterial DNA DNA extraction Nosocomial infection Arctic medicine. Tropical medicine RC955-962 |
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Polymerase chain reaction Bacterial DNA DNA extraction Nosocomial infection Arctic medicine. Tropical medicine RC955-962 Maristela Oliveira Lara Thabata Coaglio Lucas Evanguedes kalapothakis Ronaldo Luis Thomasini Carla Jorge Machado Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR |
topic_facet |
Polymerase chain reaction Bacterial DNA DNA extraction Nosocomial infection Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. RESULTS: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. CONCLUSIONS: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols. |
format |
Article in Journal/Newspaper |
author |
Maristela Oliveira Lara Thabata Coaglio Lucas Evanguedes kalapothakis Ronaldo Luis Thomasini Carla Jorge Machado |
author_facet |
Maristela Oliveira Lara Thabata Coaglio Lucas Evanguedes kalapothakis Ronaldo Luis Thomasini Carla Jorge Machado |
author_sort |
Maristela Oliveira Lara |
title |
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR |
title_short |
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR |
title_full |
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR |
title_fullStr |
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR |
title_full_unstemmed |
Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR |
title_sort |
comparison of five methods of extraction of staphylococcus aureus dna for molecular detection by pcr |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0352-2017 https://doaj.org/article/3a09a99de58d4b9dbb706920d15166ff |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 4, Pp 528-532 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000400528&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0352-2017 https://doaj.org/article/3a09a99de58d4b9dbb706920d15166ff |
op_doi |
https://doi.org/10.1590/0037-8682-0352-2017 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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