In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.)
Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dr...
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Wolters Kluwer Medknow Publications
2014
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ftdoajarticles:oai:doaj.org/article:3a009af644e6432cacc4eea2838e7148 2023-05-15T15:09:17+02:00 In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya 2014-12-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0462 https://doaj.org/article/3a009af644e6432cacc4eea2838e7148 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301118 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0462 https://doaj.org/article/3a009af644e6432cacc4eea2838e7148 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 12, Pp 959-963 (2014) Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0462 2022-12-31T03:39:34Z Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was used as the reference agent (concentrations tested: 0.012, 0.025, 0.050, 0.100 and 0.200 mg/mL). Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid (from rooster comb) and bovine testicular hyaluronidase. Results: The results revealed that BTB had moderate [IC50=(1.09±0.12) mg/mL] and dose dependent (r2=0.94) anti-hyaluronidase activity. EGCG also exhibited dose dependent (r2=0.93, P<0.05) anti-hyaluronidase activity which was superior [IC50=(0.09±0.00) mg/mL] to BTB. Conclusions: Sri Lankan low grown orthodox OP grade black tea has promising anti-hyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical. In addition, it may prove useful as a beverage in the management of allergy, some joint diseases and envenomation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 12 959 963 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
topic_facet |
Camellia sinensis Orange pekoe Black tea Sri Lankan tea Anti-aging Anti-hyaluronidase Hyaluronic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe (OP) grade black tea with a view to develop an anti-aging skin formulation. Methods: Five concentrations (0.125, 0.250, 0.500, 1.000 and 2.000 mg/mL) of black tea brew (BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification. Epigallocatechin gallate (EGCG) was used as the reference agent (concentrations tested: 0.012, 0.025, 0.050, 0.100 and 0.200 mg/mL). Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid (from rooster comb) and bovine testicular hyaluronidase. Results: The results revealed that BTB had moderate [IC50=(1.09±0.12) mg/mL] and dose dependent (r2=0.94) anti-hyaluronidase activity. EGCG also exhibited dose dependent (r2=0.93, P<0.05) anti-hyaluronidase activity which was superior [IC50=(0.09±0.00) mg/mL] to BTB. Conclusions: Sri Lankan low grown orthodox OP grade black tea has promising anti-hyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical. In addition, it may prove useful as a beverage in the management of allergy, some joint diseases and envenomation. |
format |
Article in Journal/Newspaper |
author |
Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya |
author_facet |
Wanigasekera Daya Ratnasooriya Walimuni Prabhashini Kaushalya Mendis Abeysekera Chatura Tissa Dayendra Ratnasooriya |
author_sort |
Wanigasekera Daya Ratnasooriya |
title |
In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
title_short |
In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
title_full |
In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
title_fullStr |
In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
title_full_unstemmed |
In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea (Camellia sinensis L.) |
title_sort |
in vitro anti-hyaluronidase activity of sri lankan low grown orthodox orange pekoe grade black tea (camellia sinensis l.) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0462 https://doaj.org/article/3a009af644e6432cacc4eea2838e7148 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 12, Pp 959-963 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301118 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014APJTB-2014-0462 https://doaj.org/article/3a009af644e6432cacc4eea2838e7148 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0462 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
12 |
container_start_page |
959 |
op_container_end_page |
963 |
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1766340495167979520 |