Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.)
The use of lumpfish ( Cyclopterus lumpus ) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill...
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ftdoajarticles:oai:doaj.org/article:39f702c4745d4ae4ae974ff1204a7a60 2023-10-01T03:54:47+02:00 Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) Hilde Sindre Mona C. Gjessing Johanna Hol Fosse Lene C. Hermansen Inger Böckerman Marit M. Amundsen Maria K. Dahle Anita Solhaug 2021-09-01T00:00:00Z https://doi.org/10.3390/cells10092442 https://doaj.org/article/39f702c4745d4ae4ae974ff1204a7a60 EN eng MDPI AG https://www.mdpi.com/2073-4409/10/9/2442 https://doaj.org/toc/2073-4409 doi:10.3390/cells10092442 2073-4409 https://doaj.org/article/39f702c4745d4ae4ae974ff1204a7a60 Cells, Vol 10, Iss 2442, p 2442 (2021) cleaner fish lumpfish respiration epithelial endothelial virus Cytology QH573-671 article 2021 ftdoajarticles https://doi.org/10.3390/cells10092442 2023-09-03T00:38:06Z The use of lumpfish ( Cyclopterus lumpus ) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Cells 10 9 2442 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
cleaner fish lumpfish respiration epithelial endothelial virus Cytology QH573-671 |
spellingShingle |
cleaner fish lumpfish respiration epithelial endothelial virus Cytology QH573-671 Hilde Sindre Mona C. Gjessing Johanna Hol Fosse Lene C. Hermansen Inger Böckerman Marit M. Amundsen Maria K. Dahle Anita Solhaug Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) |
topic_facet |
cleaner fish lumpfish respiration epithelial endothelial virus Cytology QH573-671 |
description |
The use of lumpfish ( Cyclopterus lumpus ) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish. |
format |
Article in Journal/Newspaper |
author |
Hilde Sindre Mona C. Gjessing Johanna Hol Fosse Lene C. Hermansen Inger Böckerman Marit M. Amundsen Maria K. Dahle Anita Solhaug |
author_facet |
Hilde Sindre Mona C. Gjessing Johanna Hol Fosse Lene C. Hermansen Inger Böckerman Marit M. Amundsen Maria K. Dahle Anita Solhaug |
author_sort |
Hilde Sindre |
title |
Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) |
title_short |
Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) |
title_full |
Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) |
title_fullStr |
Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) |
title_full_unstemmed |
Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish ( Cyclopterus lumpus L.) |
title_sort |
establishment and characterization of a novel gill cell line, lg-1, from atlantic lumpfish ( cyclopterus lumpus l.) |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/cells10092442 https://doaj.org/article/39f702c4745d4ae4ae974ff1204a7a60 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Cells, Vol 10, Iss 2442, p 2442 (2021) |
op_relation |
https://www.mdpi.com/2073-4409/10/9/2442 https://doaj.org/toc/2073-4409 doi:10.3390/cells10092442 2073-4409 https://doaj.org/article/39f702c4745d4ae4ae974ff1204a7a60 |
op_doi |
https://doi.org/10.3390/cells10092442 |
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Cells |
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10 |
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9 |
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2442 |
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1778522703732408320 |