First Description of a Satellite DNA in Manatees’ Centromeric Regions
Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and...
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2021
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ftdoajarticles:oai:doaj.org/article:39f52ef3d2624e309961ca4ece2b29db 2023-05-15T16:36:24+02:00 First Description of a Satellite DNA in Manatees’ Centromeric Regions Mirela Pelizaro Valeri Guilherme Borges Dias Alice Alves do Espírito Santo Camila Nascimento Moreira Yatiyo Yonenaga-Yassuda Iara Braga Sommer Gustavo C. S. Kuhn Marta Svartman 2021-08-01T00:00:00Z https://doi.org/10.3389/fgene.2021.694866 https://doaj.org/article/39f52ef3d2624e309961ca4ece2b29db EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fgene.2021.694866/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2021.694866 https://doaj.org/article/39f52ef3d2624e309961ca4ece2b29db Frontiers in Genetics, Vol 12 (2021) tandem repeats Trichechus manatus Trichechus inunguis chromosome mapping fluorescent in situ hybridization TAREAN Genetics QH426-470 article 2021 ftdoajarticles https://doi.org/10.3389/fgene.2021.694866 2022-12-31T12:29:14Z Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis, TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus. We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus, T. inunguis, D. dugon, and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology. Article in Journal/Newspaper Hydrodamalis gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Genetics 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
tandem repeats Trichechus manatus Trichechus inunguis chromosome mapping fluorescent in situ hybridization TAREAN Genetics QH426-470 |
spellingShingle |
tandem repeats Trichechus manatus Trichechus inunguis chromosome mapping fluorescent in situ hybridization TAREAN Genetics QH426-470 Mirela Pelizaro Valeri Guilherme Borges Dias Alice Alves do Espírito Santo Camila Nascimento Moreira Yatiyo Yonenaga-Yassuda Iara Braga Sommer Gustavo C. S. Kuhn Marta Svartman First Description of a Satellite DNA in Manatees’ Centromeric Regions |
topic_facet |
tandem repeats Trichechus manatus Trichechus inunguis chromosome mapping fluorescent in situ hybridization TAREAN Genetics QH426-470 |
description |
Trichechus manatus and Trichechus inunguis are the two Sirenia species that occur in the Americas. Despite their increasing extinction risk, many aspects of their biology remain understudied, including the repetitive DNA fraction of their genomes. Here we used the sequenced genome of T. manatus and TAREAN to identify satellite DNAs (satDNAs) in this species. We report the first description of TMAsat, a satDNA comprising ~0.87% of the genome, with ~684bp monomers and centromeric localization. In T. inunguis, TMAsat showed similar monomer length, chromosome localization and conserved CENP-B box-like motifs as in T. manatus. We also detected this satDNA in the Dugong dugon and in the now extinct Hydrodamalis gigas genomes. The neighbor-joining tree shows that TMAsat sequences from T. manatus, T. inunguis, D. dugon, and H. gigas lack species-specific clusters, which disagrees with the predictions of concerted evolution. We detected a divergent TMAsat-like homologous sequence in elephants and hyraxes, but not in other mammals, suggesting this sequence was already present in the common ancestor of Paenungulata, and later became a satDNA in the Sirenians. This is the first description of a centromeric satDNA in manatees and will facilitate the inclusion of Sirenia in future studies of centromeres and satDNA biology. |
format |
Article in Journal/Newspaper |
author |
Mirela Pelizaro Valeri Guilherme Borges Dias Alice Alves do Espírito Santo Camila Nascimento Moreira Yatiyo Yonenaga-Yassuda Iara Braga Sommer Gustavo C. S. Kuhn Marta Svartman |
author_facet |
Mirela Pelizaro Valeri Guilherme Borges Dias Alice Alves do Espírito Santo Camila Nascimento Moreira Yatiyo Yonenaga-Yassuda Iara Braga Sommer Gustavo C. S. Kuhn Marta Svartman |
author_sort |
Mirela Pelizaro Valeri |
title |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_short |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_full |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_fullStr |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_full_unstemmed |
First Description of a Satellite DNA in Manatees’ Centromeric Regions |
title_sort |
first description of a satellite dna in manatees’ centromeric regions |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fgene.2021.694866 https://doaj.org/article/39f52ef3d2624e309961ca4ece2b29db |
genre |
Hydrodamalis gigas |
genre_facet |
Hydrodamalis gigas |
op_source |
Frontiers in Genetics, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fgene.2021.694866/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2021.694866 https://doaj.org/article/39f52ef3d2624e309961ca4ece2b29db |
op_doi |
https://doi.org/10.3389/fgene.2021.694866 |
container_title |
Frontiers in Genetics |
container_volume |
12 |
_version_ |
1766026740333805568 |