Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests
Abstract Drug-resistant Plasmodium is a frequent global threat in malaria eradication programmes, highlighting the need for new anti-malarial drugs and efficient detection of treatment failure. Plasmodium falciparum culture is essential in drug discovery and resistance surveillance. Microscopy of Gi...
Published in: | Malaria Journal |
---|---|
Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2021
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-021-03591-8 https://doaj.org/article/39ec51025c304bbfafa1dc9b05df216b |
id |
ftdoajarticles:oai:doaj.org/article:39ec51025c304bbfafa1dc9b05df216b |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:39ec51025c304bbfafa1dc9b05df216b 2023-05-15T15:06:09+02:00 Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests Kasem Kulkeaw 2021-01-01T00:00:00Z https://doi.org/10.1186/s12936-021-03591-8 https://doaj.org/article/39ec51025c304bbfafa1dc9b05df216b EN eng BMC https://doi.org/10.1186/s12936-021-03591-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03591-8 1475-2875 https://doaj.org/article/39ec51025c304bbfafa1dc9b05df216b Malaria Journal, Vol 20, Iss 1, Pp 1-13 (2021) Malaria Flow cytometry Plasmodium falciparum Fluorochrome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03591-8 2022-12-31T06:27:45Z Abstract Drug-resistant Plasmodium is a frequent global threat in malaria eradication programmes, highlighting the need for new anti-malarial drugs and efficient detection of treatment failure. Plasmodium falciparum culture is essential in drug discovery and resistance surveillance. Microscopy of Giemsa-stained erythrocytes is common for determining anti-malarial effects on the intraerythrocytic development of cultured Plasmodium parasites. Giemsa-based microscopy use is conventional but laborious, and its accuracy depends largely on examiner skill. Given the availability of nucleic acid-binding fluorescent dyes and advances in flow cytometry, the use of various fluorochromes has been frequently attempted for the enumeration of parasitaemia and discrimination of P. falciparum growth in drug susceptibility assays. However, fluorochromes do not meet the requirements of being fast, simple, reliable and sensitive. Thus, this review revisits the utility of fluorochromes, notes previously reported hindrances, and highlights the challenges and opportunities for using fluorochromes in flow cytometer-based drug susceptibility tests. It aims to improve drug discovery and support a resistance surveillance system, an essential feature in combatting malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Flow cytometry Plasmodium falciparum Fluorochrome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Flow cytometry Plasmodium falciparum Fluorochrome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Kasem Kulkeaw Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
topic_facet |
Malaria Flow cytometry Plasmodium falciparum Fluorochrome Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Drug-resistant Plasmodium is a frequent global threat in malaria eradication programmes, highlighting the need for new anti-malarial drugs and efficient detection of treatment failure. Plasmodium falciparum culture is essential in drug discovery and resistance surveillance. Microscopy of Giemsa-stained erythrocytes is common for determining anti-malarial effects on the intraerythrocytic development of cultured Plasmodium parasites. Giemsa-based microscopy use is conventional but laborious, and its accuracy depends largely on examiner skill. Given the availability of nucleic acid-binding fluorescent dyes and advances in flow cytometry, the use of various fluorochromes has been frequently attempted for the enumeration of parasitaemia and discrimination of P. falciparum growth in drug susceptibility assays. However, fluorochromes do not meet the requirements of being fast, simple, reliable and sensitive. Thus, this review revisits the utility of fluorochromes, notes previously reported hindrances, and highlights the challenges and opportunities for using fluorochromes in flow cytometer-based drug susceptibility tests. It aims to improve drug discovery and support a resistance surveillance system, an essential feature in combatting malaria. |
format |
Article in Journal/Newspaper |
author |
Kasem Kulkeaw |
author_facet |
Kasem Kulkeaw |
author_sort |
Kasem Kulkeaw |
title |
Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
title_short |
Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
title_full |
Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
title_fullStr |
Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
title_full_unstemmed |
Progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
title_sort |
progress and challenges in the use of fluorescence‐based flow cytometric assays for anti‐malarial drug susceptibility tests |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03591-8 https://doaj.org/article/39ec51025c304bbfafa1dc9b05df216b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-13 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03591-8 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03591-8 1475-2875 https://doaj.org/article/39ec51025c304bbfafa1dc9b05df216b |
op_doi |
https://doi.org/10.1186/s12936-021-03591-8 |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
_version_ |
1766337813298544640 |