Inhibition study of biofilms production of Pseudomonas aeruginosa strains in the presence of propolis

• Main Authors: Lorena Abadía-Patiño, Carlos Enrique Bravo-Rodriguez, José Luís Prin, Saúl José Mosqueda-Paraqueimo
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2020
• Subjects: infecciones; antibióticos; gram negativos; antibiogramas; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic
• Online Access: https://doi.org/10.5281/zenodo.3870643; https://doaj.org/article/39db59eaf65f4065b83af3ca8e3e4180

One of the main failures of antibiotics treatments of P. aeruginosa infections is due to the production of biofilms. The objective of this work was to study the inhibition of biofilm production in Pseudomonas aeruginosa strains in the presence of propolis. Susceptibility testing and synergism were performed in all strains. Biofilm production level was determined onto nitrocellulose membranes and transmission electronic microscopy. Biofilm inhibition propolis was did on plate dilution. “Dr. Julio Rodríguez” hospital, in Cumaná, P. aeruginosa strains were isolated from different infectious processes, mainly diabetic foot. All the strains produced biofilm at different levels (60% mild, 24% moderate and 16% high). Sixty percent of the strains are producers of MBL, and 49% of AmpC. The predominant antibiotype was resistance to aminoglycosides and fluoroquinolones (XII). Seven strains were resistant’s to colistin. Biofilms treated with propolis (10 μg/ml) were almost completely inhibited. In conclusion, the propolis achieved to eradicate the in vitro biofilm production, breaking the barriers of the different mechanisms of resistance to the antibiotics expressed by the P. aeruginosa strains studied in this work