Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis
Objective: To explore the effects of recombinant human calcineurin B (rhCNB) on hepatocellular carcinoma in mice. Methods: An in vivo mouse model with hepatocellular carcinoma was established, and the mice were randomized into the rhCNB, positive control and vehicle treatments groups. Tumor growth w...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
2019
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Online Access: | https://doi.org/10.4103/1995-7645.271978 https://doaj.org/article/399e544bb5dc4dc08d34a0263efb39d6 |
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ftdoajarticles:oai:doaj.org/article:399e544bb5dc4dc08d34a0263efb39d6 2023-05-15T15:05:59+02:00 Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis Xiao-Dian Zhang Zhi-Hui He Li-Ping Zheng Guo-Hui Yi Min-Ge Lin Li-Zhen Fu Shu-Hong Tian Hong-Hai Li Yan-Da Lu Shen-Hong Gu Shao-Jiang Zheng 2019-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.271978 https://doaj.org/article/399e544bb5dc4dc08d34a0263efb39d6 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=32;epage=40;aulast=Zhang https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.271978 https://doaj.org/article/399e544bb5dc4dc08d34a0263efb39d6 Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 14, Pp 32-40 (2019) mice apoptosis calcineurin cell cycle hepatocellular carcinoma Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.4103/1995-7645.271978 2022-12-31T14:20:23Z Objective: To explore the effects of recombinant human calcineurin B (rhCNB) on hepatocellular carcinoma in mice. Methods: An in vivo mouse model with hepatocellular carcinoma was established, and the mice were randomized into the rhCNB, positive control and vehicle treatments groups. Tumor growth was assessed via bioluminescence using a small animal imaging system. Relative tumor proliferation rate and tumor growth inhibition were calculated. The expression of p53 and caspase-9 proteins in tumors were detected by immunohistochemistry. In vitro, flow cytometry was used to quantify the cell-cycle stages and rate of apoptosis. Western blotting and quantitative real-time PCR assays were used to evaluate the effects of rhCNB on protein and gene expression of CDK1, cyclin B1, p53 and caspase-9. Results: rhCNB at the higher dose significantly reduced tumor growth in vivo and caused tumor cell apoptosis in vitro. The rhCNB at the higher dose was as effective as cisplatin, and was safer. Conclusions: rhCNB has potent pro-apoptotic effects on tumor cells in vivo and in vitro and is well tolerated in vivo. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 12 14 32 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
mice apoptosis calcineurin cell cycle hepatocellular carcinoma Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
mice apoptosis calcineurin cell cycle hepatocellular carcinoma Arctic medicine. Tropical medicine RC955-962 Xiao-Dian Zhang Zhi-Hui He Li-Ping Zheng Guo-Hui Yi Min-Ge Lin Li-Zhen Fu Shu-Hong Tian Hong-Hai Li Yan-Da Lu Shen-Hong Gu Shao-Jiang Zheng Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
topic_facet |
mice apoptosis calcineurin cell cycle hepatocellular carcinoma Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To explore the effects of recombinant human calcineurin B (rhCNB) on hepatocellular carcinoma in mice. Methods: An in vivo mouse model with hepatocellular carcinoma was established, and the mice were randomized into the rhCNB, positive control and vehicle treatments groups. Tumor growth was assessed via bioluminescence using a small animal imaging system. Relative tumor proliferation rate and tumor growth inhibition were calculated. The expression of p53 and caspase-9 proteins in tumors were detected by immunohistochemistry. In vitro, flow cytometry was used to quantify the cell-cycle stages and rate of apoptosis. Western blotting and quantitative real-time PCR assays were used to evaluate the effects of rhCNB on protein and gene expression of CDK1, cyclin B1, p53 and caspase-9. Results: rhCNB at the higher dose significantly reduced tumor growth in vivo and caused tumor cell apoptosis in vitro. The rhCNB at the higher dose was as effective as cisplatin, and was safer. Conclusions: rhCNB has potent pro-apoptotic effects on tumor cells in vivo and in vitro and is well tolerated in vivo. |
format |
Article in Journal/Newspaper |
author |
Xiao-Dian Zhang Zhi-Hui He Li-Ping Zheng Guo-Hui Yi Min-Ge Lin Li-Zhen Fu Shu-Hong Tian Hong-Hai Li Yan-Da Lu Shen-Hong Gu Shao-Jiang Zheng |
author_facet |
Xiao-Dian Zhang Zhi-Hui He Li-Ping Zheng Guo-Hui Yi Min-Ge Lin Li-Zhen Fu Shu-Hong Tian Hong-Hai Li Yan-Da Lu Shen-Hong Gu Shao-Jiang Zheng |
author_sort |
Xiao-Dian Zhang |
title |
Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
title_short |
Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
title_full |
Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
title_fullStr |
Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
title_full_unstemmed |
Recombinant human calcineurin B inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
title_sort |
recombinant human calcineurin b inhibits orthotopic hepatocellular carcinoma xenograt growth in mice by promoting apoptosis |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/1995-7645.271978 https://doaj.org/article/399e544bb5dc4dc08d34a0263efb39d6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 14, Pp 32-40 (2019) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=32;epage=40;aulast=Zhang https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.271978 https://doaj.org/article/399e544bb5dc4dc08d34a0263efb39d6 |
op_doi |
https://doi.org/10.4103/1995-7645.271978 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
12 |
container_issue |
14 |
container_start_page |
32 |
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1766337654644801536 |