The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model
Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats...
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ftdoajarticles:oai:doaj.org/article:397f5c668ba6403eaa56b21eeb01e323 2023-05-15T16:01:39+02:00 The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model Budi Susilo Cahya Yustisia Hasan Bambang Dwi Raharjo Tri Wahyu Pangestiningsih 2021-04-01T00:00:00Z https://doi.org/10.32539/bsm.v5i8.338 https://doaj.org/article/397f5c668ba6403eaa56b21eeb01e323 EN eng HM Publisher https://www.bioscmed.com/index.php/bsm/article/view/338 https://doaj.org/toc/2598-0580 2598-0580 doi:10.32539/bsm.v5i8.338 https://doaj.org/article/397f5c668ba6403eaa56b21eeb01e323 Bioscientia Medicina: Journal of Biomedicine and Translational Research, Vol 5, Iss 8, Pp 717-725 (2021) diabetes mellitus type 2 low intensity pulse ultrasound (lipus) wound closure density of angiogenesis Therapeutics. Pharmacology RM1-950 Neurology. Diseases of the nervous system RC346-429 article 2021 ftdoajarticles https://doi.org/10.32539/bsm.v5i8.338 2022-12-31T08:03:33Z Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14. Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles Bioscientia Medicina : Journal of Biomedicine and Translational Research 5 8 717 725 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
diabetes mellitus type 2 low intensity pulse ultrasound (lipus) wound closure density of angiogenesis Therapeutics. Pharmacology RM1-950 Neurology. Diseases of the nervous system RC346-429 |
spellingShingle |
diabetes mellitus type 2 low intensity pulse ultrasound (lipus) wound closure density of angiogenesis Therapeutics. Pharmacology RM1-950 Neurology. Diseases of the nervous system RC346-429 Budi Susilo Cahya Yustisia Hasan Bambang Dwi Raharjo Tri Wahyu Pangestiningsih The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model |
topic_facet |
diabetes mellitus type 2 low intensity pulse ultrasound (lipus) wound closure density of angiogenesis Therapeutics. Pharmacology RM1-950 Neurology. Diseases of the nervous system RC346-429 |
description |
Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14. |
format |
Article in Journal/Newspaper |
author |
Budi Susilo Cahya Yustisia Hasan Bambang Dwi Raharjo Tri Wahyu Pangestiningsih |
author_facet |
Budi Susilo Cahya Yustisia Hasan Bambang Dwi Raharjo Tri Wahyu Pangestiningsih |
author_sort |
Budi Susilo |
title |
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model |
title_short |
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model |
title_full |
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model |
title_fullStr |
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model |
title_full_unstemmed |
The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model |
title_sort |
effect application of low intensity pulse ultra sound on wounds healing and angiogenesis of diabetes mellitus type 2 rat model |
publisher |
HM Publisher |
publishDate |
2021 |
url |
https://doi.org/10.32539/bsm.v5i8.338 https://doaj.org/article/397f5c668ba6403eaa56b21eeb01e323 |
genre |
DML |
genre_facet |
DML |
op_source |
Bioscientia Medicina: Journal of Biomedicine and Translational Research, Vol 5, Iss 8, Pp 717-725 (2021) |
op_relation |
https://www.bioscmed.com/index.php/bsm/article/view/338 https://doaj.org/toc/2598-0580 2598-0580 doi:10.32539/bsm.v5i8.338 https://doaj.org/article/397f5c668ba6403eaa56b21eeb01e323 |
op_doi |
https://doi.org/10.32539/bsm.v5i8.338 |
container_title |
Bioscientia Medicina : Journal of Biomedicine and Translational Research |
container_volume |
5 |
container_issue |
8 |
container_start_page |
717 |
op_container_end_page |
725 |
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1766397416517402624 |