SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA
The causative agent of cholera, Vibrio cholerae, can enter into a viable but non-culturable (VBNC) state in response to unfavorable conditions. The aim of this study was to evaluate the in situ survival of V. cholerae in an aquatic environment of the Southern Caribbean Sea, and its induction and res...
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ftdoajarticles:oai:doaj.org/article:396ec47495de400c988dd31da21148c2 2024-09-09T19:26:31+00:00 SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA Milagro Fernández-Delgado María Alexandra García-Amado Monica Contreras Renzo Nino Incani Humberto Chirinos Héctor Rojas Paula Suárez 2015-02-01T00:00:00Z https://doi.org/10.1590/S0036-46652015000100003 https://doaj.org/article/396ec47495de400c988dd31da21148c2 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000100021&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652015000100003 https://doaj.org/article/396ec47495de400c988dd31da21148c2 Revista do Instituto de Medicina Tropical de São Paulo, Vol 57, Iss 1, Pp 21-26 (2015) Vibrio cholera In situ survival VBNC state Resuscitation Aquatic environments Cholera Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2015 ftdoajarticles https://doi.org/10.1590/S0036-46652015000100003 2024-08-05T17:49:30Z The causative agent of cholera, Vibrio cholerae, can enter into a viable but non-culturable (VBNC) state in response to unfavorable conditions. The aim of this study was to evaluate the in situ survival of V. cholerae in an aquatic environment of the Southern Caribbean Sea, and its induction and resuscitation from the VBNC state. V. cholerae non-O1, non-O139 was inoculated into diffusion chambers placed at the Cuare Wildlife Refuge, Venezuela, and monitored for plate, total and viable cells counts. At 119 days of exposure to the environment, the colony count was < 10 CFU/mL and a portion of the bacterial population entered the VBNC state. Additionally, the viability decreased two orders of magnitude and morphological changes occurred from rod to coccoid cells. Among the aquatic environmental variables, the salinity had negative correlation with the colony counts in the dry season. Resuscitation studies showed significant recovery of cell cultivability with spent media addition (p < 0.05). These results suggest that V. cholerae can persist in the VBNC state in this Caribbean environment and revert to a cultivable form under favorable conditions. The VBNC state might represent a critical step in cholera transmission in susceptible areas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 57 1 21 26 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Vibrio cholera In situ survival VBNC state Resuscitation Aquatic environments Cholera Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Vibrio cholera In situ survival VBNC state Resuscitation Aquatic environments Cholera Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Milagro Fernández-Delgado María Alexandra García-Amado Monica Contreras Renzo Nino Incani Humberto Chirinos Héctor Rojas Paula Suárez SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA |
topic_facet |
Vibrio cholera In situ survival VBNC state Resuscitation Aquatic environments Cholera Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The causative agent of cholera, Vibrio cholerae, can enter into a viable but non-culturable (VBNC) state in response to unfavorable conditions. The aim of this study was to evaluate the in situ survival of V. cholerae in an aquatic environment of the Southern Caribbean Sea, and its induction and resuscitation from the VBNC state. V. cholerae non-O1, non-O139 was inoculated into diffusion chambers placed at the Cuare Wildlife Refuge, Venezuela, and monitored for plate, total and viable cells counts. At 119 days of exposure to the environment, the colony count was < 10 CFU/mL and a portion of the bacterial population entered the VBNC state. Additionally, the viability decreased two orders of magnitude and morphological changes occurred from rod to coccoid cells. Among the aquatic environmental variables, the salinity had negative correlation with the colony counts in the dry season. Resuscitation studies showed significant recovery of cell cultivability with spent media addition (p < 0.05). These results suggest that V. cholerae can persist in the VBNC state in this Caribbean environment and revert to a cultivable form under favorable conditions. The VBNC state might represent a critical step in cholera transmission in susceptible areas. |
format |
Article in Journal/Newspaper |
author |
Milagro Fernández-Delgado María Alexandra García-Amado Monica Contreras Renzo Nino Incani Humberto Chirinos Héctor Rojas Paula Suárez |
author_facet |
Milagro Fernández-Delgado María Alexandra García-Amado Monica Contreras Renzo Nino Incani Humberto Chirinos Héctor Rojas Paula Suárez |
author_sort |
Milagro Fernández-Delgado |
title |
SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA |
title_short |
SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA |
title_full |
SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA |
title_fullStr |
SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA |
title_full_unstemmed |
SURVIVAL, INDUCTION AND RESUSCITATION OF Vibrio cholerae FROM THE VIABLE BUT NON-CULTURABLE STATE IN THE SOUTHERN CARIBBEAN SEA |
title_sort |
survival, induction and resuscitation of vibrio cholerae from the viable but non-culturable state in the southern caribbean sea |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2015 |
url |
https://doi.org/10.1590/S0036-46652015000100003 https://doaj.org/article/396ec47495de400c988dd31da21148c2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 57, Iss 1, Pp 21-26 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000100021&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652015000100003 https://doaj.org/article/396ec47495de400c988dd31da21148c2 |
op_doi |
https://doi.org/10.1590/S0036-46652015000100003 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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57 |
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21 |
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