A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380
Cunner ( Tautogolabrus adspersus ) is a cleaner fish being considered for utilized in the North Atlantic salmon ( Salmo salar ) aquaculture industry to biocontrol sea lice infestations. However, bacterial diseases due to natural infections in wild cunners have yet to be described. This study reports...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/microorganisms9040812 https://doaj.org/article/394c595e9c014bcc9be471bec99413fa |
id |
ftdoajarticles:oai:doaj.org/article:394c595e9c014bcc9be471bec99413fa |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:394c595e9c014bcc9be471bec99413fa 2023-05-15T15:30:41+02:00 A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 Navaneethaiyer Umasuthan Katherinne Valderrama Ignacio Vasquez Cristopher Segovia Ahmed Hossain Trung Cao Hajarooba Gnanagobal Jennifer Monk Danny Boyce Javier Santander 2021-04-01T00:00:00Z https://doi.org/10.3390/microorganisms9040812 https://doaj.org/article/394c595e9c014bcc9be471bec99413fa EN eng MDPI AG https://www.mdpi.com/2076-2607/9/4/812 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9040812 2076-2607 https://doaj.org/article/394c595e9c014bcc9be471bec99413fa Microorganisms, Vol 9, Iss 812, p 812 (2021) cleaner fish Salmo salar bacterial infection comparative genomics transcriptomics iron homeostasis Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9040812 2022-12-31T05:17:00Z Cunner ( Tautogolabrus adspersus ) is a cleaner fish being considered for utilized in the North Atlantic salmon ( Salmo salar ) aquaculture industry to biocontrol sea lice infestations. However, bacterial diseases due to natural infections in wild cunners have yet to be described. This study reports the isolation of Pseudomonas sp. J380 from infected wild cunners and its phenotypic, genomic, and transcriptomic characterization. This Gram-negative motile rod-shaped bacterium showed a mesophilic (428 °C) and halotolerant growth. Under iron-limited conditions, Pseudomonas sp. J380 produced pyoverdine-type fluorescent siderophore. Koch’s postulates were verified in wild cunners by intraperitoneally (i.p.) injecting Pseudomonas sp. J380 at 4 × 10 3 , 4 × 10 5 , and 4 × 10 7 colony forming units (CFU)/dose. Host-range and comparative virulence were also investigated in lumpfish and Atlantic salmon i.p. injected with ~10 6 CFU/dose. Lumpfish were more susceptible compared to cunners, and Atlantic salmon was resistant to Pseudomonas sp. J380 infection. Cunner tissues were heavily colonized by Pseudomonas sp. J380 compared to lumpfish and Atlantic salmon suggesting that it might be an opportunistic pathogen in cunners. The genome of Pseudomonas sp. J380 was 6.26 megabases (Mb) with a guanine-cytosine (GC) content of 59.7%. Biochemical profiles, as well as comparative and phylogenomic analyses, suggested that Pseudomonas sp. J380 belongs to the P. fluorescens species complex. Transcriptome profiling under iron-limited vs. iron-enriched conditions identified 1159 differentially expressed genes (DEGs). Cellular metabolic processes, such as ribosomal and energy production, and protein synthesis, were impeded by iron limitation. In contrast, genes involved in environmental adaptation mechanisms including two-component systems, histidine catabolism, and redox balance were transcriptionally up-regulated. Furthermore, iron limitation triggered the differential expression of genes encoding proteins associated with iron ... Article in Journal/Newspaper Atlantic salmon North Atlantic Salmo salar Directory of Open Access Journals: DOAJ Articles Microorganisms 9 4 812 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
cleaner fish Salmo salar bacterial infection comparative genomics transcriptomics iron homeostasis Biology (General) QH301-705.5 |
spellingShingle |
cleaner fish Salmo salar bacterial infection comparative genomics transcriptomics iron homeostasis Biology (General) QH301-705.5 Navaneethaiyer Umasuthan Katherinne Valderrama Ignacio Vasquez Cristopher Segovia Ahmed Hossain Trung Cao Hajarooba Gnanagobal Jennifer Monk Danny Boyce Javier Santander A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 |
topic_facet |
cleaner fish Salmo salar bacterial infection comparative genomics transcriptomics iron homeostasis Biology (General) QH301-705.5 |
description |
Cunner ( Tautogolabrus adspersus ) is a cleaner fish being considered for utilized in the North Atlantic salmon ( Salmo salar ) aquaculture industry to biocontrol sea lice infestations. However, bacterial diseases due to natural infections in wild cunners have yet to be described. This study reports the isolation of Pseudomonas sp. J380 from infected wild cunners and its phenotypic, genomic, and transcriptomic characterization. This Gram-negative motile rod-shaped bacterium showed a mesophilic (428 °C) and halotolerant growth. Under iron-limited conditions, Pseudomonas sp. J380 produced pyoverdine-type fluorescent siderophore. Koch’s postulates were verified in wild cunners by intraperitoneally (i.p.) injecting Pseudomonas sp. J380 at 4 × 10 3 , 4 × 10 5 , and 4 × 10 7 colony forming units (CFU)/dose. Host-range and comparative virulence were also investigated in lumpfish and Atlantic salmon i.p. injected with ~10 6 CFU/dose. Lumpfish were more susceptible compared to cunners, and Atlantic salmon was resistant to Pseudomonas sp. J380 infection. Cunner tissues were heavily colonized by Pseudomonas sp. J380 compared to lumpfish and Atlantic salmon suggesting that it might be an opportunistic pathogen in cunners. The genome of Pseudomonas sp. J380 was 6.26 megabases (Mb) with a guanine-cytosine (GC) content of 59.7%. Biochemical profiles, as well as comparative and phylogenomic analyses, suggested that Pseudomonas sp. J380 belongs to the P. fluorescens species complex. Transcriptome profiling under iron-limited vs. iron-enriched conditions identified 1159 differentially expressed genes (DEGs). Cellular metabolic processes, such as ribosomal and energy production, and protein synthesis, were impeded by iron limitation. In contrast, genes involved in environmental adaptation mechanisms including two-component systems, histidine catabolism, and redox balance were transcriptionally up-regulated. Furthermore, iron limitation triggered the differential expression of genes encoding proteins associated with iron ... |
format |
Article in Journal/Newspaper |
author |
Navaneethaiyer Umasuthan Katherinne Valderrama Ignacio Vasquez Cristopher Segovia Ahmed Hossain Trung Cao Hajarooba Gnanagobal Jennifer Monk Danny Boyce Javier Santander |
author_facet |
Navaneethaiyer Umasuthan Katherinne Valderrama Ignacio Vasquez Cristopher Segovia Ahmed Hossain Trung Cao Hajarooba Gnanagobal Jennifer Monk Danny Boyce Javier Santander |
author_sort |
Navaneethaiyer Umasuthan |
title |
A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 |
title_short |
A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 |
title_full |
A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 |
title_fullStr |
A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 |
title_full_unstemmed |
A Novel Marine Pathogen Isolated from Wild Cunners ( Tautogolabrus adspersus ): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380 |
title_sort |
novel marine pathogen isolated from wild cunners ( tautogolabrus adspersus ): comparative genomics and transcriptome profiling of pseudomonas sp. strain j380 |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9040812 https://doaj.org/article/394c595e9c014bcc9be471bec99413fa |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_source |
Microorganisms, Vol 9, Iss 812, p 812 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/4/812 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9040812 2076-2607 https://doaj.org/article/394c595e9c014bcc9be471bec99413fa |
op_doi |
https://doi.org/10.3390/microorganisms9040812 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
4 |
container_start_page |
812 |
_version_ |
1766361134723497984 |