Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii
The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In...
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ftdoajarticles:oai:doaj.org/article:393d7f1d62b042e49fc974aa1b3c7f8d 2023-05-15T15:58:51+02:00 Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng 2022-09-01T00:00:00Z https://doi.org/10.3389/fmars.2022.1011420 https://doaj.org/article/393d7f1d62b042e49fc974aa1b3c7f8d EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.1011420/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.1011420 https://doaj.org/article/393d7f1d62b042e49fc974aa1b3c7f8d Frontiers in Marine Science, Vol 9 (2022) Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.1011420 2022-12-30T21:06:17Z The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In this study, two 5-HTR genes from Pinctada fucata martensii (Pm5-HTR2 and Pm5-HTR4) were cloned. A total of 1623 bp open reading frame was identified in Pm5-HTR2, and a 1185 bp open reading frame was detected in Pm5-HTR4; these open reading frames encoded a 540-residue polypeptide and a 394-residue polypeptide, respectively. We also conducted a domain analysis, which indicated that Pm5-HTR2 and Pm5-HTR4 contained a seven-transmembrane domain and revealed that the receptors had high similarity to Crassostrea gigas 5-HTR2 (54.62%) and 5-HTR4 (66.23%). Sequence analysis demonstrated conserved advanced structure and motifs (the DRY/ERY and NPXXY motifs). The expression pattern analysis revealed high expression levels of Pm5-HTR2 and Pm5-HTR4 during the developmental stages. ISH analysis showed that Pm5-HTR2 was primarily expressed in the FE, B, T, EU, and EL stages and Pm5-HTR4 was mainly expressed in the FE, B, T, D, EU, and EL stages. These results suggest that 5-HTRs may play key roles in P. f. martensii larval metamorphosis. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning Science Q General. Including nature conservation geographical distribution QH1-199.5 Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii |
topic_facet |
Pinctada fucata martensii Pm5-HTR2 Pm5-HTR4 larval metamorphosis molecular cloning Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The receptors of serotonin, also known as 5-hydroxytryptamine receptor (5-HTR) can mediate regulatory metamorphosis processes in a variety of mollusks. Studying the mechanisms of metamorphosis of the pearl oyster is significant to elucidate breeding, resource recovery and marine pearl production. In this study, two 5-HTR genes from Pinctada fucata martensii (Pm5-HTR2 and Pm5-HTR4) were cloned. A total of 1623 bp open reading frame was identified in Pm5-HTR2, and a 1185 bp open reading frame was detected in Pm5-HTR4; these open reading frames encoded a 540-residue polypeptide and a 394-residue polypeptide, respectively. We also conducted a domain analysis, which indicated that Pm5-HTR2 and Pm5-HTR4 contained a seven-transmembrane domain and revealed that the receptors had high similarity to Crassostrea gigas 5-HTR2 (54.62%) and 5-HTR4 (66.23%). Sequence analysis demonstrated conserved advanced structure and motifs (the DRY/ERY and NPXXY motifs). The expression pattern analysis revealed high expression levels of Pm5-HTR2 and Pm5-HTR4 during the developmental stages. ISH analysis showed that Pm5-HTR2 was primarily expressed in the FE, B, T, EU, and EL stages and Pm5-HTR4 was mainly expressed in the FE, B, T, D, EU, and EL stages. These results suggest that 5-HTRs may play key roles in P. f. martensii larval metamorphosis. |
format |
Article in Journal/Newspaper |
author |
Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng |
author_facet |
Shaojie Zhu Yubo He Qiongyu Xu Jiabin Zhang Chuangye Yang Ruijuan Hao Junhui Li Yuewen Deng |
author_sort |
Shaojie Zhu |
title |
Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii |
title_short |
Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii |
title_full |
Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii |
title_fullStr |
Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii |
title_full_unstemmed |
Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii |
title_sort |
molecular cloning, characterization, and expression of two 5-htrs from the pearl oyster pinctada fucata martensii |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.1011420 https://doaj.org/article/393d7f1d62b042e49fc974aa1b3c7f8d |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.1011420/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.1011420 https://doaj.org/article/393d7f1d62b042e49fc974aa1b3c7f8d |
op_doi |
https://doi.org/10.3389/fmars.2022.1011420 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1766394614368960512 |