Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates.
In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations.Twenty...
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ftdoajarticles:oai:doaj.org/article:392dddb81a794980b002af18622f181d 2023-05-15T15:14:48+02:00 Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. Adama Sanou Zekiba Tarnagda Estelle Kanyala Dezemon Zingué Moumini Nouctara Zakaria Ganamé Adjima Combary Hervé Hien Mathurin Dembele Antoinette Kabore Nicolas Meda Philippe Van de Perre Dorine Neveu Anne Laure Bañuls Sylvain Godreuil 2014-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003142 https://doaj.org/article/392dddb81a794980b002af18622f181d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4183478?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003142 https://doaj.org/article/392dddb81a794980b002af18622f181d PLoS Neglected Tropical Diseases, Vol 8, Iss 10, p e3142 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003142 2022-12-31T15:04:25Z In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations.Twenty six M. bovis strains were isolated from 101 cattle carcasses with suspected bTB lesions during routine meat inspections at the Bobo Dioulasso and Ouagadougou slaughterhouses. In addition, 7 M. bovis strains were isolated from 576 patients with pulmonary tuberculosis. Spoligotyping, RDAf1 deletion and MIRU-VNTR typing were used for strains genotyping. The isolation of M. bovis strains was confirmed by spoligotyping and 12 spoligotype signatures were detected. Together, the spoligotyping and MIRU-VNTR data allowed grouping the 33 M. bovis isolates in seven clusters including isolates exclusively from cattle (5) or humans (1) or from both (1). Moreover, these data (genetic analyses and phenetic tree) showed that the M. bovis isolates belonged to the African 1 (Af1) clonal complex (81.8%) and the putative African 5 (Af5) clonal complex (18.2%), in agreement with the results of RDAf1 deletion typing.This is the first detailed molecular characterization of M. bovis strains from humans and cattle in Burkina Faso. The distribution of the two Af1 and putative Af5 clonal complexes is comparable to what has been reported in neighbouring countries. Furthermore, the strain genetic profiles suggest that M. bovis circulates across the borders and that the Burkina Faso strains originate from different countries, but have a country-specific evolution. The genetic characterization suggests that, currently, M. bovis transmission occurs mainly between cattle, occasionally between cattle and humans and potentially between humans. This study emphasizes the bTB risk in cattle but also in humans and the difficulty to set up proper disease control strategies in Burkina Faso. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 10 e3142 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Adama Sanou Zekiba Tarnagda Estelle Kanyala Dezemon Zingué Moumini Nouctara Zakaria Ganamé Adjima Combary Hervé Hien Mathurin Dembele Antoinette Kabore Nicolas Meda Philippe Van de Perre Dorine Neveu Anne Laure Bañuls Sylvain Godreuil Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In sub-Saharan Africa, bovine tuberculosis (bTB) is a potential hazard for animals and humans health. The goal of this study was to improve our understanding of bTB epidemiology in Burkina Faso and especially Mycobacterium bovis transmission within and between the bovine and human populations.Twenty six M. bovis strains were isolated from 101 cattle carcasses with suspected bTB lesions during routine meat inspections at the Bobo Dioulasso and Ouagadougou slaughterhouses. In addition, 7 M. bovis strains were isolated from 576 patients with pulmonary tuberculosis. Spoligotyping, RDAf1 deletion and MIRU-VNTR typing were used for strains genotyping. The isolation of M. bovis strains was confirmed by spoligotyping and 12 spoligotype signatures were detected. Together, the spoligotyping and MIRU-VNTR data allowed grouping the 33 M. bovis isolates in seven clusters including isolates exclusively from cattle (5) or humans (1) or from both (1). Moreover, these data (genetic analyses and phenetic tree) showed that the M. bovis isolates belonged to the African 1 (Af1) clonal complex (81.8%) and the putative African 5 (Af5) clonal complex (18.2%), in agreement with the results of RDAf1 deletion typing.This is the first detailed molecular characterization of M. bovis strains from humans and cattle in Burkina Faso. The distribution of the two Af1 and putative Af5 clonal complexes is comparable to what has been reported in neighbouring countries. Furthermore, the strain genetic profiles suggest that M. bovis circulates across the borders and that the Burkina Faso strains originate from different countries, but have a country-specific evolution. The genetic characterization suggests that, currently, M. bovis transmission occurs mainly between cattle, occasionally between cattle and humans and potentially between humans. This study emphasizes the bTB risk in cattle but also in humans and the difficulty to set up proper disease control strategies in Burkina Faso. |
format |
Article in Journal/Newspaper |
author |
Adama Sanou Zekiba Tarnagda Estelle Kanyala Dezemon Zingué Moumini Nouctara Zakaria Ganamé Adjima Combary Hervé Hien Mathurin Dembele Antoinette Kabore Nicolas Meda Philippe Van de Perre Dorine Neveu Anne Laure Bañuls Sylvain Godreuil |
author_facet |
Adama Sanou Zekiba Tarnagda Estelle Kanyala Dezemon Zingué Moumini Nouctara Zakaria Ganamé Adjima Combary Hervé Hien Mathurin Dembele Antoinette Kabore Nicolas Meda Philippe Van de Perre Dorine Neveu Anne Laure Bañuls Sylvain Godreuil |
author_sort |
Adama Sanou |
title |
Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. |
title_short |
Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. |
title_full |
Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. |
title_fullStr |
Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. |
title_full_unstemmed |
Mycobacterium bovis in Burkina Faso: epidemiologic and genetic links between human and cattle isolates. |
title_sort |
mycobacterium bovis in burkina faso: epidemiologic and genetic links between human and cattle isolates. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003142 https://doaj.org/article/392dddb81a794980b002af18622f181d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 10, p e3142 (2014) |
op_relation |
http://europepmc.org/articles/PMC4183478?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003142 https://doaj.org/article/392dddb81a794980b002af18622f181d |
op_doi |
https://doi.org/10.1371/journal.pntd.0003142 |
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PLoS Neglected Tropical Diseases |
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10 |
container_start_page |
e3142 |
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