Assessing approaches to determine the effect of ocean acidification on bacterial processes
Bacterial extracellular enzymes play a significant role in the degradation of labile organic matter and nutrient availability in the open ocean. Although bacterial production and extracellular enzymes may be affected by ocean acidification, few studies to date have considered the methodology used to...
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ftdoajarticles:oai:doaj.org/article:39034c0f7a10405fb60cd4d496ed7cec 2023-05-15T17:49:25+02:00 Assessing approaches to determine the effect of ocean acidification on bacterial processes T. J. Burrell E. W. Maas P. Teesdale-Spittle C. S. Law 2016-08-01T00:00:00Z https://doi.org/10.5194/bg-13-4379-2016 https://doaj.org/article/39034c0f7a10405fb60cd4d496ed7cec EN eng Copernicus Publications http://www.biogeosciences.net/13/4379/2016/bg-13-4379-2016.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-13-4379-2016 https://doaj.org/article/39034c0f7a10405fb60cd4d496ed7cec Biogeosciences, Vol 13, Iss 15, Pp 4379-4388 (2016) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2016 ftdoajarticles https://doi.org/10.5194/bg-13-4379-2016 2022-12-30T23:57:22Z Bacterial extracellular enzymes play a significant role in the degradation of labile organic matter and nutrient availability in the open ocean. Although bacterial production and extracellular enzymes may be affected by ocean acidification, few studies to date have considered the methodology used to measure enzyme activity and bacterial processes. This study investigated the potential artefacts in determining the response of bacterial growth and extracellular glucosidase and aminopeptidase activity to ocean acidification as well as the relative effects of three different acidification techniques. Tests confirmed that the observed effect of pH on fluorescence of artificial fluorophores, and the influence of the MCA fluorescent substrate on seawater sample pH, were both overcome by the use of Tris buffer. In experiments testing different acidification methods, bubbling with CO 2 gas mixtures resulted in higher β -glucosidase activity and 15–40 % higher bacterial abundance, relative to acidification via gas-permeable silicon tubing and acid addition (HCl). Bubbling may stimulate carbohydrate degradation and bacterial growth, leading to the incorrect interpretation of the impacts of ocean acidification on organic matter cycling. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 13 15 4379 4388 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 T. J. Burrell E. W. Maas P. Teesdale-Spittle C. S. Law Assessing approaches to determine the effect of ocean acidification on bacterial processes |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Bacterial extracellular enzymes play a significant role in the degradation of labile organic matter and nutrient availability in the open ocean. Although bacterial production and extracellular enzymes may be affected by ocean acidification, few studies to date have considered the methodology used to measure enzyme activity and bacterial processes. This study investigated the potential artefacts in determining the response of bacterial growth and extracellular glucosidase and aminopeptidase activity to ocean acidification as well as the relative effects of three different acidification techniques. Tests confirmed that the observed effect of pH on fluorescence of artificial fluorophores, and the influence of the MCA fluorescent substrate on seawater sample pH, were both overcome by the use of Tris buffer. In experiments testing different acidification methods, bubbling with CO 2 gas mixtures resulted in higher β -glucosidase activity and 15–40 % higher bacterial abundance, relative to acidification via gas-permeable silicon tubing and acid addition (HCl). Bubbling may stimulate carbohydrate degradation and bacterial growth, leading to the incorrect interpretation of the impacts of ocean acidification on organic matter cycling. |
format |
Article in Journal/Newspaper |
author |
T. J. Burrell E. W. Maas P. Teesdale-Spittle C. S. Law |
author_facet |
T. J. Burrell E. W. Maas P. Teesdale-Spittle C. S. Law |
author_sort |
T. J. Burrell |
title |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_short |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_full |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_fullStr |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_full_unstemmed |
Assessing approaches to determine the effect of ocean acidification on bacterial processes |
title_sort |
assessing approaches to determine the effect of ocean acidification on bacterial processes |
publisher |
Copernicus Publications |
publishDate |
2016 |
url |
https://doi.org/10.5194/bg-13-4379-2016 https://doaj.org/article/39034c0f7a10405fb60cd4d496ed7cec |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 13, Iss 15, Pp 4379-4388 (2016) |
op_relation |
http://www.biogeosciences.net/13/4379/2016/bg-13-4379-2016.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-13-4379-2016 https://doaj.org/article/39034c0f7a10405fb60cd4d496ed7cec |
op_doi |
https://doi.org/10.5194/bg-13-4379-2016 |
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Biogeosciences |
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13 |
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15 |
container_start_page |
4379 |
op_container_end_page |
4388 |
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1766155742993186816 |