The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes.
Aedes mosquitoes can transmit dengue and several other severe vector-borne viral diseases, thereby influencing millions of people worldwide. Insects primarily control and clear the viral infections via their innate immune systems. Mitogen-Activated Protein Kinases (MAPKs) and antimicrobial peptides...
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ftdoajarticles:oai:doaj.org/article:38fe18258b7e4d5793f7746c3f24d1c2 2023-05-15T15:09:46+02:00 The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. Wen-Quan Liu Si-Qi Chen Hao-Qiang Bai Qi-Mei Wei Sheng-Nan Zhang Chen Chen Yi-Han Zhu Tang-Wei Yi Xiao-Pu Guo Si-Yuan Chen Meng-Jie Yin Chen-Feng Sun Shao-Hui Liang 2020-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008660 https://doaj.org/article/38fe18258b7e4d5793f7746c3f24d1c2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008660 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008660 https://doaj.org/article/38fe18258b7e4d5793f7746c3f24d1c2 PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008660 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008660 2022-12-31T07:36:44Z Aedes mosquitoes can transmit dengue and several other severe vector-borne viral diseases, thereby influencing millions of people worldwide. Insects primarily control and clear the viral infections via their innate immune systems. Mitogen-Activated Protein Kinases (MAPKs) and antimicrobial peptides (AMPs) are both evolutionarily conserved components of the innate immune systems. In this study, we investigated the role of MAPKs in Aedes mosquitoes following DENV infection by using genetic and pharmacological approaches. We demonstrated that knockdown of ERK, but not of JNK or p38, significantly enhances the viral replication in Aedes mosquito cells. The Ras/ERK signaling is activated in both the cells and midguts of Aedes mosquitoes following DENV infection, and thus plays a role in restricting the viral infection, as both genetic and pharmacological activation of the Ras/ERK pathway significantly decreases the viral titers. In contrast, inhibition of the Ras/ERK pathway enhances DENV infection. In addition, we identified a signaling crosstalk between the Ras/ERK pathway and DENV-induced AMPs in which defensin C participates in restricting DENV infection in Aedes mosquitoes. Our results reveal that the Ras/ERK signaling pathway couples AMPs to mediate the resistance of Aedes mosquitoes to DENV infection, which provides a new insight into understanding the crosstalk between MAPKs and AMPs in the innate immunity of mosquito vectors during the viral infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 8 e0008660 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Wen-Quan Liu Si-Qi Chen Hao-Qiang Bai Qi-Mei Wei Sheng-Nan Zhang Chen Chen Yi-Han Zhu Tang-Wei Yi Xiao-Pu Guo Si-Yuan Chen Meng-Jie Yin Chen-Feng Sun Shao-Hui Liang The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Aedes mosquitoes can transmit dengue and several other severe vector-borne viral diseases, thereby influencing millions of people worldwide. Insects primarily control and clear the viral infections via their innate immune systems. Mitogen-Activated Protein Kinases (MAPKs) and antimicrobial peptides (AMPs) are both evolutionarily conserved components of the innate immune systems. In this study, we investigated the role of MAPKs in Aedes mosquitoes following DENV infection by using genetic and pharmacological approaches. We demonstrated that knockdown of ERK, but not of JNK or p38, significantly enhances the viral replication in Aedes mosquito cells. The Ras/ERK signaling is activated in both the cells and midguts of Aedes mosquitoes following DENV infection, and thus plays a role in restricting the viral infection, as both genetic and pharmacological activation of the Ras/ERK pathway significantly decreases the viral titers. In contrast, inhibition of the Ras/ERK pathway enhances DENV infection. In addition, we identified a signaling crosstalk between the Ras/ERK pathway and DENV-induced AMPs in which defensin C participates in restricting DENV infection in Aedes mosquitoes. Our results reveal that the Ras/ERK signaling pathway couples AMPs to mediate the resistance of Aedes mosquitoes to DENV infection, which provides a new insight into understanding the crosstalk between MAPKs and AMPs in the innate immunity of mosquito vectors during the viral infection. |
format |
Article in Journal/Newspaper |
author |
Wen-Quan Liu Si-Qi Chen Hao-Qiang Bai Qi-Mei Wei Sheng-Nan Zhang Chen Chen Yi-Han Zhu Tang-Wei Yi Xiao-Pu Guo Si-Yuan Chen Meng-Jie Yin Chen-Feng Sun Shao-Hui Liang |
author_facet |
Wen-Quan Liu Si-Qi Chen Hao-Qiang Bai Qi-Mei Wei Sheng-Nan Zhang Chen Chen Yi-Han Zhu Tang-Wei Yi Xiao-Pu Guo Si-Yuan Chen Meng-Jie Yin Chen-Feng Sun Shao-Hui Liang |
author_sort |
Wen-Quan Liu |
title |
The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. |
title_short |
The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. |
title_full |
The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. |
title_fullStr |
The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. |
title_full_unstemmed |
The Ras/ERK signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in Aedes mosquitoes. |
title_sort |
ras/erk signaling pathway couples antimicrobial peptides to mediate resistance to dengue virus in aedes mosquitoes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008660 https://doaj.org/article/38fe18258b7e4d5793f7746c3f24d1c2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008660 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008660 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008660 https://doaj.org/article/38fe18258b7e4d5793f7746c3f24d1c2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008660 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
8 |
container_start_page |
e0008660 |
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1766340885900951552 |