Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.
BACKGROUND: Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 10...
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ftdoajarticles:oai:doaj.org/article:38ee369666cc4fc99072351ebb0a9bb4 2023-05-15T15:06:56+02:00 Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. Victoria Martínez-Sernández Laura Muiño María Jesús Perteguer Teresa Gárate Mercedes Mezo Marta González-Warleta Antonio Muro José Manuel Correia da Costa Fernanda Romarís Florencio M Ubeira 2011-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001376 https://doaj.org/article/38ee369666cc4fc99072351ebb0a9bb4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3210747?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001376 https://doaj.org/article/38ee369666cc4fc99072351ebb0a9bb4 PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1376 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001376 2022-12-31T14:11:15Z BACKGROUND: Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing. METHODOLOGY/PRINCIPAL FINDINGS: We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples. CONCLUSIONS/SIGNIFICANCE: The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 11 e1376 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Victoria Martínez-Sernández Laura Muiño María Jesús Perteguer Teresa Gárate Mercedes Mezo Marta González-Warleta Antonio Muro José Manuel Correia da Costa Fernanda Romarís Florencio M Ubeira Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing. METHODOLOGY/PRINCIPAL FINDINGS: We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples. CONCLUSIONS/SIGNIFICANCE: The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required. |
format |
Article in Journal/Newspaper |
author |
Victoria Martínez-Sernández Laura Muiño María Jesús Perteguer Teresa Gárate Mercedes Mezo Marta González-Warleta Antonio Muro José Manuel Correia da Costa Fernanda Romarís Florencio M Ubeira |
author_facet |
Victoria Martínez-Sernández Laura Muiño María Jesús Perteguer Teresa Gárate Mercedes Mezo Marta González-Warleta Antonio Muro José Manuel Correia da Costa Fernanda Romarís Florencio M Ubeira |
author_sort |
Victoria Martínez-Sernández |
title |
Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
title_short |
Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
title_full |
Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
title_fullStr |
Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
title_full_unstemmed |
Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
title_sort |
development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001376 https://doaj.org/article/38ee369666cc4fc99072351ebb0a9bb4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1376 (2011) |
op_relation |
http://europepmc.org/articles/PMC3210747?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001376 https://doaj.org/article/38ee369666cc4fc99072351ebb0a9bb4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001376 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
11 |
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e1376 |
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