Rickettsial Disease in the Peruvian Amazon Basin.
Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:38a0e45506654d05af57265ceac73032 2023-05-15T15:06:25+02:00 Rickettsial Disease in the Peruvian Amazon Basin. Claudine Kocher Amy C Morrison Mariana Leguia Steev Loyola Roger M Castillo Hugo A Galvez Helvio Astete Carmen Flores-Mendoza Julia S Ampuero Daniel G Bausch Eric S Halsey Manuel Cespedes Karine Zevallos Ju Jiang Allen L Richards 2016-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004843 https://doaj.org/article/38a0e45506654d05af57265ceac73032 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4944934?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004843 https://doaj.org/article/38a0e45506654d05af57265ceac73032 PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004843 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004843 2022-12-30T23:21:17Z Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens from humans and animals were assayed for spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR) by ELISA and/or PCR; ectoparasites were screened by PCR. Logistic regression was used to determine associations between patient history, demographic characteristics of participants and symptoms, clinical findings and outcome of rickettsial infection. Of the 2,054 enrolled participants, almost 2% showed evidence of seroconversion or a 4-fold rise in antibody titers specific for rickettsiae between acute and convalescent blood samples. Of 190 fleas (Ctenocephalides felis) and 60 ticks (Rhipicephalus sanguineus) tested, 185 (97.4%) and 3 (5%), respectively, were positive for Rickettsia spp. Candidatus Rickettsia asemboensis was identified in 100% and 33% of the fleas and ticks tested, respectively. Collectively, our serologic data indicates that human pathogenic SFGR are present in the Peruvian Amazon and pose a significant risk of infection to individuals exposed to wild, domestic and peri-domestic animals and their ectoparasites. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 7 e0004843 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Claudine Kocher Amy C Morrison Mariana Leguia Steev Loyola Roger M Castillo Hugo A Galvez Helvio Astete Carmen Flores-Mendoza Julia S Ampuero Daniel G Bausch Eric S Halsey Manuel Cespedes Karine Zevallos Ju Jiang Allen L Richards Rickettsial Disease in the Peruvian Amazon Basin. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Using a large, passive, clinic-based surveillance program in Iquitos, Peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. Blood specimens from humans and animals were assayed for spotted fever group rickettsiae (SFGR) and typhus group rickettsiae (TGR) by ELISA and/or PCR; ectoparasites were screened by PCR. Logistic regression was used to determine associations between patient history, demographic characteristics of participants and symptoms, clinical findings and outcome of rickettsial infection. Of the 2,054 enrolled participants, almost 2% showed evidence of seroconversion or a 4-fold rise in antibody titers specific for rickettsiae between acute and convalescent blood samples. Of 190 fleas (Ctenocephalides felis) and 60 ticks (Rhipicephalus sanguineus) tested, 185 (97.4%) and 3 (5%), respectively, were positive for Rickettsia spp. Candidatus Rickettsia asemboensis was identified in 100% and 33% of the fleas and ticks tested, respectively. Collectively, our serologic data indicates that human pathogenic SFGR are present in the Peruvian Amazon and pose a significant risk of infection to individuals exposed to wild, domestic and peri-domestic animals and their ectoparasites. |
format |
Article in Journal/Newspaper |
author |
Claudine Kocher Amy C Morrison Mariana Leguia Steev Loyola Roger M Castillo Hugo A Galvez Helvio Astete Carmen Flores-Mendoza Julia S Ampuero Daniel G Bausch Eric S Halsey Manuel Cespedes Karine Zevallos Ju Jiang Allen L Richards |
author_facet |
Claudine Kocher Amy C Morrison Mariana Leguia Steev Loyola Roger M Castillo Hugo A Galvez Helvio Astete Carmen Flores-Mendoza Julia S Ampuero Daniel G Bausch Eric S Halsey Manuel Cespedes Karine Zevallos Ju Jiang Allen L Richards |
author_sort |
Claudine Kocher |
title |
Rickettsial Disease in the Peruvian Amazon Basin. |
title_short |
Rickettsial Disease in the Peruvian Amazon Basin. |
title_full |
Rickettsial Disease in the Peruvian Amazon Basin. |
title_fullStr |
Rickettsial Disease in the Peruvian Amazon Basin. |
title_full_unstemmed |
Rickettsial Disease in the Peruvian Amazon Basin. |
title_sort |
rickettsial disease in the peruvian amazon basin. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004843 https://doaj.org/article/38a0e45506654d05af57265ceac73032 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004843 (2016) |
op_relation |
http://europepmc.org/articles/PMC4944934?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004843 https://doaj.org/article/38a0e45506654d05af57265ceac73032 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004843 |
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PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
7 |
container_start_page |
e0004843 |
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