Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites.
Plasmodium falciparum and Plasmodium vivax cause the majority of human malaria cases. Research efforts predominantly focus on P. falciparum because of the clinical severity of infection and associated mortality rates. However, P. vivax malaria affects more people in a wider global range. Furthermore...
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ftdoajarticles:oai:doaj.org/article:3893574e7ba44319831b3cd61e68b2d9 2023-05-15T15:16:01+02:00 Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. Kristian E Swearingen Scott E Lindner Erika L Flannery Ashley M Vaughan Robert D Morrison Rapatbhorn Patrapuvich Cristian Koepfli Ivo Muller Aaron Jex Robert L Moritz Stefan H I Kappe Jetsumon Sattabongkot Sebastian A Mikolajczak 2017-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005791 https://doaj.org/article/3893574e7ba44319831b3cd61e68b2d9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5552340?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005791 https://doaj.org/article/3893574e7ba44319831b3cd61e68b2d9 PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005791 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005791 2022-12-30T23:10:30Z Plasmodium falciparum and Plasmodium vivax cause the majority of human malaria cases. Research efforts predominantly focus on P. falciparum because of the clinical severity of infection and associated mortality rates. However, P. vivax malaria affects more people in a wider global range. Furthermore, unlike P. falciparum, P. vivax can persist in the liver as dormant hypnozoites that can be activated weeks to years after primary infection, causing relapse of symptomatic blood stages. This feature makes P. vivax unique and difficult to eliminate with the standard tools of vector control and treatment of symptomatic blood stage infection with antimalarial drugs. Infection by Plasmodium is initiated by the mosquito-transmitted sporozoite stage, a highly motile invasive cell that targets hepatocytes in the liver. The most advanced malaria vaccine for P. falciparum (RTS,S, a subunit vaccine containing of a portion of the major sporozoite surface protein) conferred limited protection in Phase III trials, falling short of WHO-established vaccine efficacy goals. However, blocking the sporozoite stage of infection in P. vivax, before the establishment of the chronic liver infection, might be an effective malaria vaccine strategy to reduce the occurrence of relapsing blood stages. It is also thought that a multivalent vaccine comprising multiple sporozoite surface antigens will provide better protection, but a comprehensive analysis of proteins in P. vivax sporozoites is not available. To inform sporozoite-based vaccine development, we employed mass spectrometry-based proteomics to identify nearly 2,000 proteins present in P. vivax salivary gland sporozoites. Analysis of protein post-translational modifications revealed extensive phosphorylation of glideosome proteins as well as regulators of transcription and translation. Additionally, the sporozoite surface proteins CSP and TRAP, which were recently discovered to be glycosylated in P. falciparum salivary gland sporozoites, were also observed to be similarly modified in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 7 e0005791 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kristian E Swearingen Scott E Lindner Erika L Flannery Ashley M Vaughan Robert D Morrison Rapatbhorn Patrapuvich Cristian Koepfli Ivo Muller Aaron Jex Robert L Moritz Stefan H I Kappe Jetsumon Sattabongkot Sebastian A Mikolajczak Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Plasmodium falciparum and Plasmodium vivax cause the majority of human malaria cases. Research efforts predominantly focus on P. falciparum because of the clinical severity of infection and associated mortality rates. However, P. vivax malaria affects more people in a wider global range. Furthermore, unlike P. falciparum, P. vivax can persist in the liver as dormant hypnozoites that can be activated weeks to years after primary infection, causing relapse of symptomatic blood stages. This feature makes P. vivax unique and difficult to eliminate with the standard tools of vector control and treatment of symptomatic blood stage infection with antimalarial drugs. Infection by Plasmodium is initiated by the mosquito-transmitted sporozoite stage, a highly motile invasive cell that targets hepatocytes in the liver. The most advanced malaria vaccine for P. falciparum (RTS,S, a subunit vaccine containing of a portion of the major sporozoite surface protein) conferred limited protection in Phase III trials, falling short of WHO-established vaccine efficacy goals. However, blocking the sporozoite stage of infection in P. vivax, before the establishment of the chronic liver infection, might be an effective malaria vaccine strategy to reduce the occurrence of relapsing blood stages. It is also thought that a multivalent vaccine comprising multiple sporozoite surface antigens will provide better protection, but a comprehensive analysis of proteins in P. vivax sporozoites is not available. To inform sporozoite-based vaccine development, we employed mass spectrometry-based proteomics to identify nearly 2,000 proteins present in P. vivax salivary gland sporozoites. Analysis of protein post-translational modifications revealed extensive phosphorylation of glideosome proteins as well as regulators of transcription and translation. Additionally, the sporozoite surface proteins CSP and TRAP, which were recently discovered to be glycosylated in P. falciparum salivary gland sporozoites, were also observed to be similarly modified in ... |
format |
Article in Journal/Newspaper |
author |
Kristian E Swearingen Scott E Lindner Erika L Flannery Ashley M Vaughan Robert D Morrison Rapatbhorn Patrapuvich Cristian Koepfli Ivo Muller Aaron Jex Robert L Moritz Stefan H I Kappe Jetsumon Sattabongkot Sebastian A Mikolajczak |
author_facet |
Kristian E Swearingen Scott E Lindner Erika L Flannery Ashley M Vaughan Robert D Morrison Rapatbhorn Patrapuvich Cristian Koepfli Ivo Muller Aaron Jex Robert L Moritz Stefan H I Kappe Jetsumon Sattabongkot Sebastian A Mikolajczak |
author_sort |
Kristian E Swearingen |
title |
Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. |
title_short |
Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. |
title_full |
Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. |
title_fullStr |
Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. |
title_full_unstemmed |
Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites. |
title_sort |
proteogenomic analysis of the total and surface-exposed proteomes of plasmodium vivax salivary gland sporozoites. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005791 https://doaj.org/article/3893574e7ba44319831b3cd61e68b2d9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005791 (2017) |
op_relation |
http://europepmc.org/articles/PMC5552340?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005791 https://doaj.org/article/3893574e7ba44319831b3cd61e68b2d9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005791 |
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PLOS Neglected Tropical Diseases |
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11 |
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7 |
container_start_page |
e0005791 |
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