The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics
Abstract Background An increasing number of studies are addressing the evolutionary genomics of dog domestication, principally through resequencing dog, wolf and related canid genomes. There is, however, only one de novo assembled canid genome currently available against which to map such data - tha...
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ftdoajarticles:oai:doaj.org/article:388e9aa0267a435cb585411d9b51b973 2023-05-15T15:49:26+02:00 The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics Shyam Gopalakrishnan Jose A. Samaniego Castruita Mikkel-Holger S. Sinding Lukas F. K. Kuderna Jannikke Räikkönen Bent Petersen Thomas Sicheritz-Ponten Greger Larson Ludovic Orlando Tomas Marques-Bonet Anders J. Hansen Love Dalén M. Thomas P. Gilbert 2017-06-01T00:00:00Z https://doi.org/10.1186/s12864-017-3883-3 https://doaj.org/article/388e9aa0267a435cb585411d9b51b973 EN eng BMC http://link.springer.com/article/10.1186/s12864-017-3883-3 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-017-3883-3 1471-2164 https://doaj.org/article/388e9aa0267a435cb585411d9b51b973 BMC Genomics, Vol 18, Iss 1, Pp 1-11 (2017) Canis lupus Evolutionary genomics Genome Wolf Biotechnology TP248.13-248.65 Genetics QH426-470 article 2017 ftdoajarticles https://doi.org/10.1186/s12864-017-3883-3 2022-12-31T01:50:34Z Abstract Background An increasing number of studies are addressing the evolutionary genomics of dog domestication, principally through resequencing dog, wolf and related canid genomes. There is, however, only one de novo assembled canid genome currently available against which to map such data - that of a boxer dog (Canis lupus familiaris). We generated the first de novo wolf genome (Canis lupus lupus) as an additional choice of reference, and explored what implications may arise when previously published dog and wolf resequencing data are remapped to this reference. Results Reassuringly, we find that regardless of the reference genome choice, most evolutionary genomic analyses yield qualitatively similar results, including those exploring the structure between the wolves and dogs using admixture and principal component analysis. However, we do observe differences in the genomic coverage of re-mapped samples, the number of variants discovered, and heterozygosity estimates of the samples. Conclusion In conclusion, the choice of reference is dictated by the aims of the study being undertaken; if the study focuses on the differences between the different dog breeds or the fine structure among dogs, then using the boxer reference genome is appropriate, but if the aim of the study is to look at the variation within wolves and their relationships to dogs, then there are clear benefits to using the de novo assembled wolf reference genome. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles BMC Genomics 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Canis lupus Evolutionary genomics Genome Wolf Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Canis lupus Evolutionary genomics Genome Wolf Biotechnology TP248.13-248.65 Genetics QH426-470 Shyam Gopalakrishnan Jose A. Samaniego Castruita Mikkel-Holger S. Sinding Lukas F. K. Kuderna Jannikke Räikkönen Bent Petersen Thomas Sicheritz-Ponten Greger Larson Ludovic Orlando Tomas Marques-Bonet Anders J. Hansen Love Dalén M. Thomas P. Gilbert The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics |
topic_facet |
Canis lupus Evolutionary genomics Genome Wolf Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background An increasing number of studies are addressing the evolutionary genomics of dog domestication, principally through resequencing dog, wolf and related canid genomes. There is, however, only one de novo assembled canid genome currently available against which to map such data - that of a boxer dog (Canis lupus familiaris). We generated the first de novo wolf genome (Canis lupus lupus) as an additional choice of reference, and explored what implications may arise when previously published dog and wolf resequencing data are remapped to this reference. Results Reassuringly, we find that regardless of the reference genome choice, most evolutionary genomic analyses yield qualitatively similar results, including those exploring the structure between the wolves and dogs using admixture and principal component analysis. However, we do observe differences in the genomic coverage of re-mapped samples, the number of variants discovered, and heterozygosity estimates of the samples. Conclusion In conclusion, the choice of reference is dictated by the aims of the study being undertaken; if the study focuses on the differences between the different dog breeds or the fine structure among dogs, then using the boxer reference genome is appropriate, but if the aim of the study is to look at the variation within wolves and their relationships to dogs, then there are clear benefits to using the de novo assembled wolf reference genome. |
format |
Article in Journal/Newspaper |
author |
Shyam Gopalakrishnan Jose A. Samaniego Castruita Mikkel-Holger S. Sinding Lukas F. K. Kuderna Jannikke Räikkönen Bent Petersen Thomas Sicheritz-Ponten Greger Larson Ludovic Orlando Tomas Marques-Bonet Anders J. Hansen Love Dalén M. Thomas P. Gilbert |
author_facet |
Shyam Gopalakrishnan Jose A. Samaniego Castruita Mikkel-Holger S. Sinding Lukas F. K. Kuderna Jannikke Räikkönen Bent Petersen Thomas Sicheritz-Ponten Greger Larson Ludovic Orlando Tomas Marques-Bonet Anders J. Hansen Love Dalén M. Thomas P. Gilbert |
author_sort |
Shyam Gopalakrishnan |
title |
The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics |
title_short |
The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics |
title_full |
The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics |
title_fullStr |
The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics |
title_full_unstemmed |
The wolf reference genome sequence (Canis lupus lupus) and its implications for Canis spp. population genomics |
title_sort |
wolf reference genome sequence (canis lupus lupus) and its implications for canis spp. population genomics |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12864-017-3883-3 https://doaj.org/article/388e9aa0267a435cb585411d9b51b973 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
BMC Genomics, Vol 18, Iss 1, Pp 1-11 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12864-017-3883-3 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-017-3883-3 1471-2164 https://doaj.org/article/388e9aa0267a435cb585411d9b51b973 |
op_doi |
https://doi.org/10.1186/s12864-017-3883-3 |
container_title |
BMC Genomics |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766384464822272000 |