Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides
Abstract Several phenolic compounds derived from plant biomass are attracting attention because they display high antioxidant activity. In this study, antioxidant activity was confirmed in 4-hydroxyphenylacetic acid (HPA), and transesterification reaction using Candida antarctica lipase B was perfor...
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ftdoajarticles:oai:doaj.org/article:387c07e70e6448d1993caace8d8e868b 2023-05-15T13:38:29+02:00 Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides Mustika Sari Yustine Chung Felicia Agatha Hyung Kwoun Kim 2019-02-01T00:00:00Z https://doi.org/10.1186/s13765-019-0419-3 https://doaj.org/article/387c07e70e6448d1993caace8d8e868b EN eng SpringerOpen http://link.springer.com/article/10.1186/s13765-019-0419-3 https://doaj.org/toc/2468-0834 https://doaj.org/toc/2468-0842 doi:10.1186/s13765-019-0419-3 2468-0834 2468-0842 https://doaj.org/article/387c07e70e6448d1993caace8d8e868b Applied Biological Chemistry, Vol 62, Iss 1, Pp 1-9 (2019) Lipase Phenolic lipid Antioxidant Antibacterial activity Agriculture (General) S1-972 Chemistry QD1-999 article 2019 ftdoajarticles https://doi.org/10.1186/s13765-019-0419-3 2022-12-30T22:20:48Z Abstract Several phenolic compounds derived from plant biomass are attracting attention because they display high antioxidant activity. In this study, antioxidant activity was confirmed in 4-hydroxyphenylacetic acid (HPA), and transesterification reaction using Candida antarctica lipase B was performed to enhance the solubility of HPA. The HPA-diolein (HPA-DON) was synthesized from HPA and triolein, while the HPA-fish oil diglyceride (HPA-dFO) was synthesized from HPA and Menhaden fish oil. To increase the conversion yield, the enzyme reaction conditions of the substrate molar ratio, enzyme amount, and reaction time were optimized. After the reaction, HPA-DON and HPA-dFO were purely separated, using prep-LC. The activity assays using DPPH and ABTS radicals confirmed that HPA-DON and HPA-dFO have antioxidant activity. HPA-DON has high activity in non-polar solvents, while HPA-dFO has strong activity in both polar solvents and non-polar solvents. In addition, HPA-dFO has the growth inhibition activity for Bacillus coagulans, Geobacillus stearothermophilus, and Alcaligenes faecalis that cause food spoilage. Therefore, HPA-dFO is a new synthetic substance that has both antioxidant activity and antibacterial activity. The results indicate that these HPA-derivatives can be expected to be developed as important materials in the food and cosmetics industries. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Applied Biological Chemistry 62 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Lipase Phenolic lipid Antioxidant Antibacterial activity Agriculture (General) S1-972 Chemistry QD1-999 |
spellingShingle |
Lipase Phenolic lipid Antioxidant Antibacterial activity Agriculture (General) S1-972 Chemistry QD1-999 Mustika Sari Yustine Chung Felicia Agatha Hyung Kwoun Kim Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
topic_facet |
Lipase Phenolic lipid Antioxidant Antibacterial activity Agriculture (General) S1-972 Chemistry QD1-999 |
description |
Abstract Several phenolic compounds derived from plant biomass are attracting attention because they display high antioxidant activity. In this study, antioxidant activity was confirmed in 4-hydroxyphenylacetic acid (HPA), and transesterification reaction using Candida antarctica lipase B was performed to enhance the solubility of HPA. The HPA-diolein (HPA-DON) was synthesized from HPA and triolein, while the HPA-fish oil diglyceride (HPA-dFO) was synthesized from HPA and Menhaden fish oil. To increase the conversion yield, the enzyme reaction conditions of the substrate molar ratio, enzyme amount, and reaction time were optimized. After the reaction, HPA-DON and HPA-dFO were purely separated, using prep-LC. The activity assays using DPPH and ABTS radicals confirmed that HPA-DON and HPA-dFO have antioxidant activity. HPA-DON has high activity in non-polar solvents, while HPA-dFO has strong activity in both polar solvents and non-polar solvents. In addition, HPA-dFO has the growth inhibition activity for Bacillus coagulans, Geobacillus stearothermophilus, and Alcaligenes faecalis that cause food spoilage. Therefore, HPA-dFO is a new synthetic substance that has both antioxidant activity and antibacterial activity. The results indicate that these HPA-derivatives can be expected to be developed as important materials in the food and cosmetics industries. |
format |
Article in Journal/Newspaper |
author |
Mustika Sari Yustine Chung Felicia Agatha Hyung Kwoun Kim |
author_facet |
Mustika Sari Yustine Chung Felicia Agatha Hyung Kwoun Kim |
author_sort |
Mustika Sari |
title |
Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
title_short |
Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
title_full |
Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
title_fullStr |
Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
title_full_unstemmed |
Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
title_sort |
evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides |
publisher |
SpringerOpen |
publishDate |
2019 |
url |
https://doi.org/10.1186/s13765-019-0419-3 https://doaj.org/article/387c07e70e6448d1993caace8d8e868b |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Applied Biological Chemistry, Vol 62, Iss 1, Pp 1-9 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s13765-019-0419-3 https://doaj.org/toc/2468-0834 https://doaj.org/toc/2468-0842 doi:10.1186/s13765-019-0419-3 2468-0834 2468-0842 https://doaj.org/article/387c07e70e6448d1993caace8d8e868b |
op_doi |
https://doi.org/10.1186/s13765-019-0419-3 |
container_title |
Applied Biological Chemistry |
container_volume |
62 |
container_issue |
1 |
_version_ |
1766106650910916608 |