New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load.
Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out t...
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ftdoajarticles:oai:doaj.org/article:385f0d36da8b450cb06c020476760606 2023-05-15T15:07:12+02:00 New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. Rafaella Grenfell Donald A Harn Smanla Tundup Akram Da'dara Liliane Siqueira Paulo Marcos Zech Coelho 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002054 https://doaj.org/article/385f0d36da8b450cb06c020476760606 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3585039?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002054 https://doaj.org/article/385f0d36da8b450cb06c020476760606 PLoS Neglected Tropical Diseases, Vol 7, Iss 2, p e2054 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002054 2022-12-30T22:30:04Z Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our previous investigations, the use of crude antigens led to false-positive results. Recently, focus has been given to highly purified Schistosoma mansoni antigens, especially to circulating antigens.Thus, our main goal was to test different types of circulating cathodic antigen glycoprotein (CCA), as "crude antigen," the protein chain of recombinant CCA and two individual peptides. These schistosome proteins/peptides were tested in a new diagnostic method employing immunomagnetic separation based on the improvement of antigen-antibody binding.Use of recombinant CCA as a diagnostic antigen allowed us to develop a diagnostic assay with high sensitivity and specificity with no false-negative results. Interestingly, the "crude antigen" worked as a good marker for control of cure after praziquantel treatment.Our new diagnostic method was superior to enzyme-linked immunosorbent assay in diagnosing low endemicity patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 2 e2054 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rafaella Grenfell Donald A Harn Smanla Tundup Akram Da'dara Liliane Siqueira Paulo Marcos Zech Coelho New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our previous investigations, the use of crude antigens led to false-positive results. Recently, focus has been given to highly purified Schistosoma mansoni antigens, especially to circulating antigens.Thus, our main goal was to test different types of circulating cathodic antigen glycoprotein (CCA), as "crude antigen," the protein chain of recombinant CCA and two individual peptides. These schistosome proteins/peptides were tested in a new diagnostic method employing immunomagnetic separation based on the improvement of antigen-antibody binding.Use of recombinant CCA as a diagnostic antigen allowed us to develop a diagnostic assay with high sensitivity and specificity with no false-negative results. Interestingly, the "crude antigen" worked as a good marker for control of cure after praziquantel treatment.Our new diagnostic method was superior to enzyme-linked immunosorbent assay in diagnosing low endemicity patients. |
format |
Article in Journal/Newspaper |
author |
Rafaella Grenfell Donald A Harn Smanla Tundup Akram Da'dara Liliane Siqueira Paulo Marcos Zech Coelho |
author_facet |
Rafaella Grenfell Donald A Harn Smanla Tundup Akram Da'dara Liliane Siqueira Paulo Marcos Zech Coelho |
author_sort |
Rafaella Grenfell |
title |
New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. |
title_short |
New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. |
title_full |
New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. |
title_fullStr |
New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. |
title_full_unstemmed |
New approaches with different types of circulating cathodic antigen for the diagnosis of patients with low Schistosoma mansoni load. |
title_sort |
new approaches with different types of circulating cathodic antigen for the diagnosis of patients with low schistosoma mansoni load. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002054 https://doaj.org/article/385f0d36da8b450cb06c020476760606 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 2, p e2054 (2013) |
op_relation |
http://europepmc.org/articles/PMC3585039?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002054 https://doaj.org/article/385f0d36da8b450cb06c020476760606 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002054 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
2 |
container_start_page |
e2054 |
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1766338757912428544 |