The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases
Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a vari...
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ftdoajarticles:oai:doaj.org/article:3824161299714a6798113bcd3ebfee08 2024-02-04T09:58:51+01:00 The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases Matilde Mengkrog Holen Tina Rise Tuveng Matthew Peter Kent Gustav Vaaje‐Kolstad 2024-01-01T00:00:00Z https://doi.org/10.1002/2211-5463.13694 https://doaj.org/article/3824161299714a6798113bcd3ebfee08 EN eng Wiley https://doi.org/10.1002/2211-5463.13694 https://doaj.org/toc/2211-5463 2211-5463 doi:10.1002/2211-5463.13694 https://doaj.org/article/3824161299714a6798113bcd3ebfee08 FEBS Open Bio, Vol 14, Iss 1, Pp 23-36 (2024) Atlantic salmon chitin chitinase gastric mucosa Biology (General) QH301-705.5 article 2024 ftdoajarticles https://doi.org/10.1002/2211-5463.13694 2024-01-07T01:42:16Z Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a variety of non‐digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa. Chia.3 and Chia.4, exhibiting 95% sequence identity, proved inseparable using conventional chromatographic methods, necessitating their purification as a chitinase pair. Biochemical analysis revealed sustained chitinolytic activity against β‐chitin for up to 24 h, spanning a pH range of 2 to 6. Moreover, subsequent in vitro investigations established that this chitinase pair efficiently degrades diverse chitin‐containing substrates into chitobiose, highlighting the potential of Atlantic salmon to utilize novel chitin‐containing feed sources. Analysis of the gastric matrix proteome demonstrates that the chitinases are secreted and rank among the most abundant proteins in the gastric matrix. This finding correlates well with the previously observed high transcription of the corresponding chitinase genes in Atlantic salmon stomach tissue. By shedding light on the secreted chitinases in the Atlantic salmon's stomach mucosa and elucidating their functional characteristics, this study enhances our understanding of chitinase biology in this species. Moreover, the observed capacity to effectively degrade chitin‐containing materials implies the potential utilization of alternative feed sources rich in chitin, offering promising prospects for sustainable aquaculture practices. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles FEBS Open Bio 14 1 23 36 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
Atlantic salmon chitin chitinase gastric mucosa Biology (General) QH301-705.5 |
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Atlantic salmon chitin chitinase gastric mucosa Biology (General) QH301-705.5 Matilde Mengkrog Holen Tina Rise Tuveng Matthew Peter Kent Gustav Vaaje‐Kolstad The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases |
topic_facet |
Atlantic salmon chitin chitinase gastric mucosa Biology (General) QH301-705.5 |
description |
Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase‐encoding genes are transcribed in a variety of non‐digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa. Chia.3 and Chia.4, exhibiting 95% sequence identity, proved inseparable using conventional chromatographic methods, necessitating their purification as a chitinase pair. Biochemical analysis revealed sustained chitinolytic activity against β‐chitin for up to 24 h, spanning a pH range of 2 to 6. Moreover, subsequent in vitro investigations established that this chitinase pair efficiently degrades diverse chitin‐containing substrates into chitobiose, highlighting the potential of Atlantic salmon to utilize novel chitin‐containing feed sources. Analysis of the gastric matrix proteome demonstrates that the chitinases are secreted and rank among the most abundant proteins in the gastric matrix. This finding correlates well with the previously observed high transcription of the corresponding chitinase genes in Atlantic salmon stomach tissue. By shedding light on the secreted chitinases in the Atlantic salmon's stomach mucosa and elucidating their functional characteristics, this study enhances our understanding of chitinase biology in this species. Moreover, the observed capacity to effectively degrade chitin‐containing materials implies the potential utilization of alternative feed sources rich in chitin, offering promising prospects for sustainable aquaculture practices. |
format |
Article in Journal/Newspaper |
author |
Matilde Mengkrog Holen Tina Rise Tuveng Matthew Peter Kent Gustav Vaaje‐Kolstad |
author_facet |
Matilde Mengkrog Holen Tina Rise Tuveng Matthew Peter Kent Gustav Vaaje‐Kolstad |
author_sort |
Matilde Mengkrog Holen |
title |
The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases |
title_short |
The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases |
title_full |
The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases |
title_fullStr |
The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases |
title_full_unstemmed |
The gastric mucosa of Atlantic salmon (Salmo salar) is abundant in highly active chitinases |
title_sort |
gastric mucosa of atlantic salmon (salmo salar) is abundant in highly active chitinases |
publisher |
Wiley |
publishDate |
2024 |
url |
https://doi.org/10.1002/2211-5463.13694 https://doaj.org/article/3824161299714a6798113bcd3ebfee08 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
FEBS Open Bio, Vol 14, Iss 1, Pp 23-36 (2024) |
op_relation |
https://doi.org/10.1002/2211-5463.13694 https://doaj.org/toc/2211-5463 2211-5463 doi:10.1002/2211-5463.13694 https://doaj.org/article/3824161299714a6798113bcd3ebfee08 |
op_doi |
https://doi.org/10.1002/2211-5463.13694 |
container_title |
FEBS Open Bio |
container_volume |
14 |
container_issue |
1 |
container_start_page |
23 |
op_container_end_page |
36 |
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1789963439149416448 |