Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia
Objective: To determine the cytotoxicity, reduction in nitric oxide production and anti-oxidative activity of the aqueous leaf extract from Tithonia diversifolia (T. diversifolia) in an in vitro model. Methods: Leaves of T. diversifolia were collected from natural habitats and extracted with distill...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2016
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Online Access: | https://doi.org/10.1016/j.apjtb.2016.02.002 https://doaj.org/article/37fe819d5f4f4f4e8378114bc65c132e |
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ftdoajarticles:oai:doaj.org/article:37fe819d5f4f4f4e8378114bc65c132e 2023-05-15T15:15:32+02:00 Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia Poonsit Hiransai Jitbanjong Tangpong Chuthamat Kumbuar Namon Hoonheang Onrunee Rodpech Padchara Sangsuk Urairat Kajklangdon Waraphorn Inkaow 2016-11-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.02.002 https://doaj.org/article/37fe819d5f4f4f4e8378114bc65c132e EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116307900 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.02.002 https://doaj.org/article/37fe819d5f4f4f4e8378114bc65c132e Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 11, Pp 950-956 (2016) Tithonia diversifolia Anti-nitric oxide production Anti-proliferation Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.02.002 2022-12-31T15:11:00Z Objective: To determine the cytotoxicity, reduction in nitric oxide production and anti-oxidative activity of the aqueous leaf extract from Tithonia diversifolia (T. diversifolia) in an in vitro model. Methods: Leaves of T. diversifolia were collected from natural habitats and extracted with distilled water using the decoction method. The cytotoxic effect of the extract in terms of cell viability was determined using RAW264.7 cells and human peripheral blood mononuclear cells (PBMCs) via the mitochondrial respiration method using the MTT reagent. The effect of the extract on lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells was measured using the Griess reagent. The chemical antioxidant was evaluated by ABTS- and DPPH-radical scavenging assays. Results: The half-maximal cytotoxic concentration values were 145.87 μg/mL and 73.67 μg/mL for human PBMCs and RAW264.7 cells, respectively. In the presence of phytohemagglutinin-M, the IC50 on PBMCs proliferation was 4.42 μg/mL. The non-cytotoxic range of the extracts inhibited LPS-induced nitrite production in RAW264.7 cells with an IC50 value of 11.63 μg/mL. To determine the anti-oxidative properties, the N-acetyl cysteine equivalent antioxidant capacity of the extract was (32.62 ± 1.87) and (20.99 ± 2.79) mg N-acetyl cysteine/g extract, respectively determined by the ABTS-radical and DPPH-radical assay. However, the extract did not confer death protection in a hydrogen peroxide-induced RAW264.7 co-culturing model. Conclusions: Our study demonstrated the immunomodulation caused by the aqueous leaf extract of T. diversifolia, resulting from the inhibition of phytohemagglutinin-M-induced PBMCs proliferation and LPS-induced nitric oxide production in RAW264.7 macrophages. Although the anti-oxidative activity was presented in the chemical-based anti-oxidant assay, the extract cannot protect cell death from stress conditions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 11 950 956 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tithonia diversifolia Anti-nitric oxide production Anti-proliferation Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Tithonia diversifolia Anti-nitric oxide production Anti-proliferation Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Poonsit Hiransai Jitbanjong Tangpong Chuthamat Kumbuar Namon Hoonheang Onrunee Rodpech Padchara Sangsuk Urairat Kajklangdon Waraphorn Inkaow Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia |
topic_facet |
Tithonia diversifolia Anti-nitric oxide production Anti-proliferation Antioxidant Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the cytotoxicity, reduction in nitric oxide production and anti-oxidative activity of the aqueous leaf extract from Tithonia diversifolia (T. diversifolia) in an in vitro model. Methods: Leaves of T. diversifolia were collected from natural habitats and extracted with distilled water using the decoction method. The cytotoxic effect of the extract in terms of cell viability was determined using RAW264.7 cells and human peripheral blood mononuclear cells (PBMCs) via the mitochondrial respiration method using the MTT reagent. The effect of the extract on lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells was measured using the Griess reagent. The chemical antioxidant was evaluated by ABTS- and DPPH-radical scavenging assays. Results: The half-maximal cytotoxic concentration values were 145.87 μg/mL and 73.67 μg/mL for human PBMCs and RAW264.7 cells, respectively. In the presence of phytohemagglutinin-M, the IC50 on PBMCs proliferation was 4.42 μg/mL. The non-cytotoxic range of the extracts inhibited LPS-induced nitrite production in RAW264.7 cells with an IC50 value of 11.63 μg/mL. To determine the anti-oxidative properties, the N-acetyl cysteine equivalent antioxidant capacity of the extract was (32.62 ± 1.87) and (20.99 ± 2.79) mg N-acetyl cysteine/g extract, respectively determined by the ABTS-radical and DPPH-radical assay. However, the extract did not confer death protection in a hydrogen peroxide-induced RAW264.7 co-culturing model. Conclusions: Our study demonstrated the immunomodulation caused by the aqueous leaf extract of T. diversifolia, resulting from the inhibition of phytohemagglutinin-M-induced PBMCs proliferation and LPS-induced nitric oxide production in RAW264.7 macrophages. Although the anti-oxidative activity was presented in the chemical-based anti-oxidant assay, the extract cannot protect cell death from stress conditions. |
format |
Article in Journal/Newspaper |
author |
Poonsit Hiransai Jitbanjong Tangpong Chuthamat Kumbuar Namon Hoonheang Onrunee Rodpech Padchara Sangsuk Urairat Kajklangdon Waraphorn Inkaow |
author_facet |
Poonsit Hiransai Jitbanjong Tangpong Chuthamat Kumbuar Namon Hoonheang Onrunee Rodpech Padchara Sangsuk Urairat Kajklangdon Waraphorn Inkaow |
author_sort |
Poonsit Hiransai |
title |
Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia |
title_short |
Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia |
title_full |
Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia |
title_fullStr |
Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia |
title_full_unstemmed |
Anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from Tithonia diversifolia |
title_sort |
anti-nitric oxide production, anti-proliferation and antioxidant effects of the aqueous extract from tithonia diversifolia |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.02.002 https://doaj.org/article/37fe819d5f4f4f4e8378114bc65c132e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 11, Pp 950-956 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116307900 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.02.002 https://doaj.org/article/37fe819d5f4f4f4e8378114bc65c132e |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.02.002 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
11 |
container_start_page |
950 |
op_container_end_page |
956 |
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1766345900185092096 |