Flavonoid chemical composition and antidiabetic potential of Brachychiton acerifolius leaves extract

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Aisha Hussein Abou Zeid, Mohamed Ali Farag, Manal Abdel Aziz Hamed, Zeinab Abdel Aziz Kandil, Radwa Hassan El-Akad, Hanaa Mohamed El-Rafie
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: STZ-induced diabetes; Diaion fractionation; Flavonoid glucuronides; Brachychiton acerifolius; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2017.01.009; https://doaj.org/article/37ef76358c294618a7e34fd6ecef4fa7

Objective: To evaluate Brachychiton acerifolius leaf extracts as antidiabetic potential agent and to identify the main active constituents using bioactivity guided fractionation. Methods: In vitro antioxidant activity was evaluated for B. acerifolius different extracts using DPPH assay and vitamin C as control. Antidiabetic activity was then determined using STZ-induced rats treated daily with ethyl acetate and 70% ethanol leaf extracts for 4 weeks at a dose of 200 g/kg body weight against gliclazide reference drug. Blood glucose, α-amylase, lipid profile, liver function enzymes and oxidative stress markers were assessed along with histopathological study for liver and pancreatic tissues. Isolation and structural elucidation of active compounds were made using Diaion and Sephadex followed by spectral analyses. Results: The results indicated that ethyl acetate and ethanol leaf extracts exhibited the strongest antioxidant activity compared to that of vitamin C (IC50 0.05, 0.03 and 12 mg/mL, respectively). Both extracts showed potent anti-hyperglycemic activity evidenced by a significant decrease in serum glucose levels by 82.5% and 80.9% and α-amylase by 45.2% and 53.6%, as compared with gliclazide 68% and 59.4%, respectively. Fractionation of ethanol extract resulted in the isolation of 9 flavonoids including apigenin-7-O-α-rhamnosyl(1→2)-β-d-glucuronide, apigenin-7-O-β-d-glucuronide, apigenin-7-O-β-d-glucoside and luteolin-7-O-β-d-glucuronide. Conclusions: This study highlights the potential use of B. acerifolius leaf extract enriched in flavones for the treatment of diabetes that would warrant further clinical trials investigation.