The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata )
The gilthead seabream larval rearing in continuous light is common in most Mediterranean hatcheries to stimulate larval length growth and increase food consumption. Several studies have shown that continuous light affects larval development and increases the prevalence of skeletal deformities. Melat...
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ftdoajarticles:oai:doaj.org/article:37ed66b851bb4482ad4516af9666203b 2023-05-15T15:32:35+02:00 The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) Kamel Mhalhel Antonino Germanà Francesco Abbate Maria Cristina Guerrera Maria Levanti Rosaria Laurà Giuseppe Montalbano 2020-12-01T00:00:00Z https://doi.org/10.3390/ijms21249597 https://doaj.org/article/37ed66b851bb4482ad4516af9666203b EN eng MDPI AG https://www.mdpi.com/1422-0067/21/24/9597 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21249597 1422-0067 1661-6596 https://doaj.org/article/37ed66b851bb4482ad4516af9666203b International Journal of Molecular Sciences, Vol 21, Iss 9597, p 9597 (2020) melatonin opercular complex bone deformity growth Sparus aurata PTHrP Biology (General) QH301-705.5 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/ijms21249597 2022-12-31T04:48:48Z The gilthead seabream larval rearing in continuous light is common in most Mediterranean hatcheries to stimulate larval length growth and increase food consumption. Several studies have shown that continuous light affects larval development and increases the prevalence of skeletal deformities. Melatonin is a crucial pineal neurohormone that displays daily secretion patterns, stimulates cell proliferation and embryonic development in Atlantic salmon and zebrafish, and improves osseointegration in mice and humans. However, no studies have examined the effects of orally supplemented melatonin on skeletal deformities in Sparus aurata larvae. We administered exogenous melatonin to gilthead seabream larvae via enriched rotifers and nauplii of Artemia. Exogenous melatonin induced bone deformities and stimulated parathyroid hormone-related protein-coding gene ( PTHrP ) mRNA expression. In addition to the melatonin-induced PTHrP high expression level, the recorded non coordinated function of skeletal muscle and bone during growth can be the fountainhead of bone deformities. Both myosin light chain 2 ( mlc2 ) and bone gamma-carboxyglutamate protein-coding gene ( bglap ) expression levels were significantly affected by melatonin administration in an inverse dose–response manner during the exogenous melatonin administration. This is the first study to report the effect of inducing melatonin bone deformities on Sparus aurata larvae reared under ordinary hatchery conditions. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 21 24 9597 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
melatonin opercular complex bone deformity growth Sparus aurata PTHrP Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
melatonin opercular complex bone deformity growth Sparus aurata PTHrP Biology (General) QH301-705.5 Chemistry QD1-999 Kamel Mhalhel Antonino Germanà Francesco Abbate Maria Cristina Guerrera Maria Levanti Rosaria Laurà Giuseppe Montalbano The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) |
topic_facet |
melatonin opercular complex bone deformity growth Sparus aurata PTHrP Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The gilthead seabream larval rearing in continuous light is common in most Mediterranean hatcheries to stimulate larval length growth and increase food consumption. Several studies have shown that continuous light affects larval development and increases the prevalence of skeletal deformities. Melatonin is a crucial pineal neurohormone that displays daily secretion patterns, stimulates cell proliferation and embryonic development in Atlantic salmon and zebrafish, and improves osseointegration in mice and humans. However, no studies have examined the effects of orally supplemented melatonin on skeletal deformities in Sparus aurata larvae. We administered exogenous melatonin to gilthead seabream larvae via enriched rotifers and nauplii of Artemia. Exogenous melatonin induced bone deformities and stimulated parathyroid hormone-related protein-coding gene ( PTHrP ) mRNA expression. In addition to the melatonin-induced PTHrP high expression level, the recorded non coordinated function of skeletal muscle and bone during growth can be the fountainhead of bone deformities. Both myosin light chain 2 ( mlc2 ) and bone gamma-carboxyglutamate protein-coding gene ( bglap ) expression levels were significantly affected by melatonin administration in an inverse dose–response manner during the exogenous melatonin administration. This is the first study to report the effect of inducing melatonin bone deformities on Sparus aurata larvae reared under ordinary hatchery conditions. |
format |
Article in Journal/Newspaper |
author |
Kamel Mhalhel Antonino Germanà Francesco Abbate Maria Cristina Guerrera Maria Levanti Rosaria Laurà Giuseppe Montalbano |
author_facet |
Kamel Mhalhel Antonino Germanà Francesco Abbate Maria Cristina Guerrera Maria Levanti Rosaria Laurà Giuseppe Montalbano |
author_sort |
Kamel Mhalhel |
title |
The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) |
title_short |
The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) |
title_full |
The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) |
title_fullStr |
The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) |
title_full_unstemmed |
The Effect of Orally Supplemented Melatonin on Larval Performance and Skeletal Deformities in Farmed Gilthead Seabream ( Sparus aurata ) |
title_sort |
effect of orally supplemented melatonin on larval performance and skeletal deformities in farmed gilthead seabream ( sparus aurata ) |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21249597 https://doaj.org/article/37ed66b851bb4482ad4516af9666203b |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
International Journal of Molecular Sciences, Vol 21, Iss 9597, p 9597 (2020) |
op_relation |
https://www.mdpi.com/1422-0067/21/24/9597 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21249597 1422-0067 1661-6596 https://doaj.org/article/37ed66b851bb4482ad4516af9666203b |
op_doi |
https://doi.org/10.3390/ijms21249597 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
24 |
container_start_page |
9597 |
_version_ |
1766363083970707456 |