Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia.
Urban Norway rats (Rattus norvegicus) can carry various human pathogens, and may be involved in pathogen propagation and transmission to humans. From January 31-August 14, 2021, a community outbreak of Shigella flexneri serotype 2a occurred among unhoused or poorly housed people in the Downtown East...
Published in: | PLOS Neglected Tropical Diseases |
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2023
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Online Access: | https://doi.org/10.1371/journal.pntd.0011669 https://doaj.org/article/37a7b8dfeded44b183aa908fdf04a918 |
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ftdoajarticles:oai:doaj.org/article:37a7b8dfeded44b183aa908fdf04a918 2023-12-03T10:18:20+01:00 Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. Lisa K F Lee Chelsea G Himsworth Kaylee A Byers Harveen K Atwal Gus Gabaldon Gordon Ritchie Christopher F Lowe Nancy Matic Samuel Chorlton Linda Hoang Bruce K Wobeser Victor Leung 2023-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011669 https://doaj.org/article/37a7b8dfeded44b183aa908fdf04a918 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011669&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011669 https://doaj.org/article/37a7b8dfeded44b183aa908fdf04a918 PLoS Neglected Tropical Diseases, Vol 17, Iss 10, p e0011669 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011669 2023-11-05T01:40:11Z Urban Norway rats (Rattus norvegicus) can carry various human pathogens, and may be involved in pathogen propagation and transmission to humans. From January 31-August 14, 2021, a community outbreak of Shigella flexneri serotype 2a occurred among unhoused or poorly housed people in the Downtown Eastside neighborhood of Vancouver, British Columbia, Canada. The source could not be identified; however, patients reported contact with rats, and previous studies indicated transmission of rat-associated zoonotic pathogens among the unhoused or poorly housed residents of this neighborhood. The study objective was to determine if rats trapped in the outbreak area were carriers of Shigella spp. and other zoonotic enteric pathogens. From March 23-April 9, 2021, 22 rats were lethally trapped within the outbreak area. Colonic content was analyzed using the BioFire FilmArray Gastrointestinal (multiplex PCR) panel for human enteropathogens, which detected: Campylobacter spp. (9/22), Clostridioides difficile (3/22), Yersinia enterocolitica (5/22), Cryptosporidium spp. (8/22), Giardia duodenalis (5/22), Rotavirus A (1/22), enteroaggressive Escherichia coli (2/22), enteropathogenic E. coli (10/22), and Shigella spp. or enteroinvasive E. coli (EIEC) (3/22). An ipaH PCR assay was used for targeted detection of Shigella spp./EIEC, with five rats positive. Two samples contained insertion sites unique to S. flexneri isolated from the human outbreak. This study highlights the potential for rats to carry a broad range of human pathogens, and their possible role in pathogen maintenance and/or transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) Canada Norway PLOS Neglected Tropical Diseases 17 10 e0011669 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lisa K F Lee Chelsea G Himsworth Kaylee A Byers Harveen K Atwal Gus Gabaldon Gordon Ritchie Christopher F Lowe Nancy Matic Samuel Chorlton Linda Hoang Bruce K Wobeser Victor Leung Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Urban Norway rats (Rattus norvegicus) can carry various human pathogens, and may be involved in pathogen propagation and transmission to humans. From January 31-August 14, 2021, a community outbreak of Shigella flexneri serotype 2a occurred among unhoused or poorly housed people in the Downtown Eastside neighborhood of Vancouver, British Columbia, Canada. The source could not be identified; however, patients reported contact with rats, and previous studies indicated transmission of rat-associated zoonotic pathogens among the unhoused or poorly housed residents of this neighborhood. The study objective was to determine if rats trapped in the outbreak area were carriers of Shigella spp. and other zoonotic enteric pathogens. From March 23-April 9, 2021, 22 rats were lethally trapped within the outbreak area. Colonic content was analyzed using the BioFire FilmArray Gastrointestinal (multiplex PCR) panel for human enteropathogens, which detected: Campylobacter spp. (9/22), Clostridioides difficile (3/22), Yersinia enterocolitica (5/22), Cryptosporidium spp. (8/22), Giardia duodenalis (5/22), Rotavirus A (1/22), enteroaggressive Escherichia coli (2/22), enteropathogenic E. coli (10/22), and Shigella spp. or enteroinvasive E. coli (EIEC) (3/22). An ipaH PCR assay was used for targeted detection of Shigella spp./EIEC, with five rats positive. Two samples contained insertion sites unique to S. flexneri isolated from the human outbreak. This study highlights the potential for rats to carry a broad range of human pathogens, and their possible role in pathogen maintenance and/or transmission. |
format |
Article in Journal/Newspaper |
author |
Lisa K F Lee Chelsea G Himsworth Kaylee A Byers Harveen K Atwal Gus Gabaldon Gordon Ritchie Christopher F Lowe Nancy Matic Samuel Chorlton Linda Hoang Bruce K Wobeser Victor Leung |
author_facet |
Lisa K F Lee Chelsea G Himsworth Kaylee A Byers Harveen K Atwal Gus Gabaldon Gordon Ritchie Christopher F Lowe Nancy Matic Samuel Chorlton Linda Hoang Bruce K Wobeser Victor Leung |
author_sort |
Lisa K F Lee |
title |
Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. |
title_short |
Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. |
title_full |
Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. |
title_fullStr |
Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. |
title_full_unstemmed |
Detection of multiple human enteropathogens in Norway rats (Rattus norvegicus) from an under-resourced neighborhood of Vancouver, British Columbia. |
title_sort |
detection of multiple human enteropathogens in norway rats (rattus norvegicus) from an under-resourced neighborhood of vancouver, british columbia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011669 https://doaj.org/article/37a7b8dfeded44b183aa908fdf04a918 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) |
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Arctic British Columbia Canada Norway |
geographic_facet |
Arctic British Columbia Canada Norway |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 10, p e0011669 (2023) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011669&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011669 https://doaj.org/article/37a7b8dfeded44b183aa908fdf04a918 |
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https://doi.org/10.1371/journal.pntd.0011669 |
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PLOS Neglected Tropical Diseases |
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