Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements
Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Four...
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ftdoajarticles:oai:doaj.org/article:379987ab256645e2ac19a812fd109294 2023-05-15T13:53:42+02:00 Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements Renaud Berlemont Delphine Pipers Maud Delsaute Federico Angiono Georges Feller Moreno Galleni Pablo Power 2011-06-01T00:00:00Z https://doaj.org/article/379987ab256645e2ac19a812fd109294 EN ES eng spa Elsevier España http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412011000200005 https://doaj.org/toc/0325-7541 https://doaj.org/toc/1851-7617 0325-7541 1851-7617 https://doaj.org/article/379987ab256645e2ac19a812fd109294 Revista Argentina de Microbiología, Vol 43, Iss 2, Pp 94-103 (2011) Metagenómica Celulasas Enzimas psicrofílicas Integrasas Metagenómica funcional Metagenomics Cellulases Psychrophilic enzymes Integrases Activity-driven metagenomics Infectious and parasitic diseases RC109-216 Microbiology QR1-502 article 2011 ftdoajarticles 2022-12-31T08:25:15Z Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Fourteen lipase/esterase-, 14 amylase-, 3 protease-, and 11 cellulase-producing clones were detected by activity-driven screening, with apparent maximum activities around 35 °C for both amylolytic and lipolytic enzymes, and 35-55 °C for cellulases, as observed for other cold-adapted enzymes. However, the behavior of at least one of the studied cellulases is more compatible to that observed for mesophilic enzymes. These enzymes are usually still active at temperatures above 60 °C, probably resulting in a psychrotolerant behavior in Antarctic soils. Metagenomics allows to access novel genes encoding for enzymatic and biophysic properties from almost every environment with potential benefits for biotechnological and industrial applications. Only intI- and tnp-like genes were detected by PCR, encoding for proteins with 58-86 %, and 58-73 % amino acid identity with known entries, respectively. Two clones, BAC 27A-9 and BAC 14A-5, seem to present unique syntenic organizations, suggesting the occurrence of gene rearrangements that were probably due to evolutionary divergences within the genus or facilitated by the association with transposable elements. The evidence for genetic elements related to recruitment and mobilization of genes (transposons/integrons) in an extreme environment like Antarctica reinforces the hypothesis of the origin of some of the genes disseminated by mobile elements among "human-associated" microorganisms. A partir de muestras de suelo antártico se obtuvo la metagenoteca PP1. Esta fue sometida a análisis funcionales y genotípicos para el aislamiento de nuevas enzimas adaptadas al frío con potenciales aplicaciones, y para la detección de elementos génicos asociados a la movilización de genes, ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Metagenómica Celulasas Enzimas psicrofílicas Integrasas Metagenómica funcional Metagenomics Cellulases Psychrophilic enzymes Integrases Activity-driven metagenomics Infectious and parasitic diseases RC109-216 Microbiology QR1-502 |
spellingShingle |
Metagenómica Celulasas Enzimas psicrofílicas Integrasas Metagenómica funcional Metagenomics Cellulases Psychrophilic enzymes Integrases Activity-driven metagenomics Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Renaud Berlemont Delphine Pipers Maud Delsaute Federico Angiono Georges Feller Moreno Galleni Pablo Power Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
topic_facet |
Metagenómica Celulasas Enzimas psicrofílicas Integrasas Metagenómica funcional Metagenomics Cellulases Psychrophilic enzymes Integrases Activity-driven metagenomics Infectious and parasitic diseases RC109-216 Microbiology QR1-502 |
description |
Metagenomic library PP1 was obtained from Antarctic soil samples. Both functional and genotypic metagenomic screening were used for the isolation of novel cold-adapted enzymes with potential applications, and for the detection of genetic elements associated with gene mobilization, respectively. Fourteen lipase/esterase-, 14 amylase-, 3 protease-, and 11 cellulase-producing clones were detected by activity-driven screening, with apparent maximum activities around 35 °C for both amylolytic and lipolytic enzymes, and 35-55 °C for cellulases, as observed for other cold-adapted enzymes. However, the behavior of at least one of the studied cellulases is more compatible to that observed for mesophilic enzymes. These enzymes are usually still active at temperatures above 60 °C, probably resulting in a psychrotolerant behavior in Antarctic soils. Metagenomics allows to access novel genes encoding for enzymatic and biophysic properties from almost every environment with potential benefits for biotechnological and industrial applications. Only intI- and tnp-like genes were detected by PCR, encoding for proteins with 58-86 %, and 58-73 % amino acid identity with known entries, respectively. Two clones, BAC 27A-9 and BAC 14A-5, seem to present unique syntenic organizations, suggesting the occurrence of gene rearrangements that were probably due to evolutionary divergences within the genus or facilitated by the association with transposable elements. The evidence for genetic elements related to recruitment and mobilization of genes (transposons/integrons) in an extreme environment like Antarctica reinforces the hypothesis of the origin of some of the genes disseminated by mobile elements among "human-associated" microorganisms. A partir de muestras de suelo antártico se obtuvo la metagenoteca PP1. Esta fue sometida a análisis funcionales y genotípicos para el aislamiento de nuevas enzimas adaptadas al frío con potenciales aplicaciones, y para la detección de elementos génicos asociados a la movilización de genes, ... |
format |
Article in Journal/Newspaper |
author |
Renaud Berlemont Delphine Pipers Maud Delsaute Federico Angiono Georges Feller Moreno Galleni Pablo Power |
author_facet |
Renaud Berlemont Delphine Pipers Maud Delsaute Federico Angiono Georges Feller Moreno Galleni Pablo Power |
author_sort |
Renaud Berlemont |
title |
Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
title_short |
Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
title_full |
Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
title_fullStr |
Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
title_full_unstemmed |
Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
title_sort |
exploring the antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements |
publisher |
Elsevier España |
publishDate |
2011 |
url |
https://doaj.org/article/379987ab256645e2ac19a812fd109294 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Revista Argentina de Microbiología, Vol 43, Iss 2, Pp 94-103 (2011) |
op_relation |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412011000200005 https://doaj.org/toc/0325-7541 https://doaj.org/toc/1851-7617 0325-7541 1851-7617 https://doaj.org/article/379987ab256645e2ac19a812fd109294 |
_version_ |
1766259083173691392 |