Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin
The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure−activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and...
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ftdoajarticles:oai:doaj.org/article:379797848ab94a6698cacf0433f86f91 2023-05-15T13:09:21+02:00 Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin Wenkui Song Qianru Chen Ying Wang Yan Han Hongwei Zhang Bo Li Guangli Yu 2019-07-01T00:00:00Z https://doi.org/10.3390/md17080450 https://doaj.org/article/379797848ab94a6698cacf0433f86f91 EN eng MDPI AG https://www.mdpi.com/1660-3397/17/8/450 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md17080450 https://doaj.org/article/379797848ab94a6698cacf0433f86f91 Marine Drugs, Vol 17, Iss 8, p 450 (2019) collagen peptide intestinal epithelium barrier function intestinal health structure–activity relationship Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.3390/md17080450 2022-12-30T23:40:19Z The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure−activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and pharmaceuticals development. In this work, size exclusion chromatography, cation-exchange chromatography, and RP-HPLC were used to separate Alaska pollock skin-derived collagen hydrolysates based on their molecular weight, charge property, and hydrophobicity. The intestinal epithelial barrier function (IEBF) protective effect of separated peptide fractions were evaluated by tumor necrosis factor (TNF)-α-induced Caco-2 cell model. Results indicated that lower molecular weight (500−1000 Da) and higher hydrophilicity of CPs were related to better IEBF protective effect. Two high-efficiency IEBF protective peptide sequences, GPSGPQGSR and GPSGLLGPK with the corresponding molecular weights of 841.41 Da and 824.38 Da, were subsequently identified by UPLC-QToF-MS/MS. Their IEBF protective ability are comparable or even better than the currently used intestinal health supplements glutamine and arginine. The present findings suggested that the hydrophilic CPs, with molecular weight between 500 Da to 1000 Da, should be preferred in IEBF protective peptides preparation. GPSGPQGSR and GPSGLLGPK might have the potential of being IEBF protective ingredients used in intestinal health supplements and drugs. Article in Journal/Newspaper alaska pollock Alaska Directory of Open Access Journals: DOAJ Articles Marine Drugs 17 8 450 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
collagen peptide intestinal epithelium barrier function intestinal health structure–activity relationship Biology (General) QH301-705.5 |
spellingShingle |
collagen peptide intestinal epithelium barrier function intestinal health structure–activity relationship Biology (General) QH301-705.5 Wenkui Song Qianru Chen Ying Wang Yan Han Hongwei Zhang Bo Li Guangli Yu Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
topic_facet |
collagen peptide intestinal epithelium barrier function intestinal health structure–activity relationship Biology (General) QH301-705.5 |
description |
The effect of collagen peptides (CPs) in intestinal mucosal protection has been approved in both cell and animal models. However, its structure−activity relationship and efficient peptide sequences are unclear, which hinders the in-depth study of its action mechanism and relative nutraceuticals and pharmaceuticals development. In this work, size exclusion chromatography, cation-exchange chromatography, and RP-HPLC were used to separate Alaska pollock skin-derived collagen hydrolysates based on their molecular weight, charge property, and hydrophobicity. The intestinal epithelial barrier function (IEBF) protective effect of separated peptide fractions were evaluated by tumor necrosis factor (TNF)-α-induced Caco-2 cell model. Results indicated that lower molecular weight (500−1000 Da) and higher hydrophilicity of CPs were related to better IEBF protective effect. Two high-efficiency IEBF protective peptide sequences, GPSGPQGSR and GPSGLLGPK with the corresponding molecular weights of 841.41 Da and 824.38 Da, were subsequently identified by UPLC-QToF-MS/MS. Their IEBF protective ability are comparable or even better than the currently used intestinal health supplements glutamine and arginine. The present findings suggested that the hydrophilic CPs, with molecular weight between 500 Da to 1000 Da, should be preferred in IEBF protective peptides preparation. GPSGPQGSR and GPSGLLGPK might have the potential of being IEBF protective ingredients used in intestinal health supplements and drugs. |
format |
Article in Journal/Newspaper |
author |
Wenkui Song Qianru Chen Ying Wang Yan Han Hongwei Zhang Bo Li Guangli Yu |
author_facet |
Wenkui Song Qianru Chen Ying Wang Yan Han Hongwei Zhang Bo Li Guangli Yu |
author_sort |
Wenkui Song |
title |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_short |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_full |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_fullStr |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_full_unstemmed |
Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin |
title_sort |
identification and structure–activity relationship of intestinal epithelial barrier function protective collagen peptides from alaska pollock skin |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/md17080450 https://doaj.org/article/379797848ab94a6698cacf0433f86f91 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_source |
Marine Drugs, Vol 17, Iss 8, p 450 (2019) |
op_relation |
https://www.mdpi.com/1660-3397/17/8/450 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md17080450 https://doaj.org/article/379797848ab94a6698cacf0433f86f91 |
op_doi |
https://doi.org/10.3390/md17080450 |
container_title |
Marine Drugs |
container_volume |
17 |
container_issue |
8 |
container_start_page |
450 |
_version_ |
1766173764859461632 |