Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela
Introduction: The treatment of urinary tract infections has become more challenging due to the increasing frequency of multidrug-resistant Escherichia coli in human populations. Objective: To characterize multidrug-resistant E. coli isolates causing community-acquired urinary tract infections in Cum...
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ftdoajarticles:oai:doaj.org/article:375ff93a78a340eba1bacf2a80d21407 2023-05-15T15:12:10+02:00 Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela Militza Guzmán Elsa Salazar Vicmaris Cordero Ana Castro Andreína Villanueva Hectorina Rodulfo Marcos De Donato 2019-05-01T00:00:00Z https://doi.org/10.7705/biomedica.v39i2.4030 https://doaj.org/article/375ff93a78a340eba1bacf2a80d21407 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/4030 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v39i2.4030 https://doaj.org/article/375ff93a78a340eba1bacf2a80d21407 Biomédica: revista del Instituto Nacional de Salud, Vol 39, Pp 96-107 (2019) Escherichia coli urologic diseases beta-lactamases drug resistance bacterial community-acquired infections Medicine R Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.7705/biomedica.v39i2.4030 2022-12-31T00:47:17Z Introduction: The treatment of urinary tract infections has become more challenging due to the increasing frequency of multidrug-resistant Escherichia coli in human populations. Objective: To characterize multidrug-resistant E. coli isolates causing community-acquired urinary tract infections in Cumaná, Venezuela, and associate possible risk factors for infection by extended-spectrum beta-lactamases (ESBL)-producing isolates. Materials and methods: We included all the patients with urinary tract infections attending the urology outpatient consultation and emergency unit in the Hospital de Cumaná, Estado Sucre, Venezuela, from January through June, 2014. blaTEM, blaSHV and blaCTX-M genes detection was carried out by PCR. Results: We found a high prevalence of multidrug-resistant E. coli (25.2%) with 20.4% of the isolates producing ESBL. The ESBL-producing isolates showed a high frequency (66.7%) of simultaneous resistance to trimethoprim-sulphamethoxazole, fluoroquinolones and aminoglycosides compared to non-producing isolates (2.4%). Of the resistant isolates, 65.4% carried the blaTEM gene, 34.6% the blaCTX-M and 23.1% the blaSHV. The blaCTX-M genes detected belonged to the CTX-M-1 and CTX-M-2 groups. Plasmid transfer was demonstrated by in vitro conjugation in 17 of the 26 ESBL-producing isolates. All three genes detected were transferred to the transconjugants. Age over 60 years, complicated urinary tract infections and previous use of a catheter predisposed patients to infection by ESBL-producing E. coli. Conclusions: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 39 96 107 |
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ftdoajarticles |
language |
English Spanish |
topic |
Escherichia coli urologic diseases beta-lactamases drug resistance bacterial community-acquired infections Medicine R Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Escherichia coli urologic diseases beta-lactamases drug resistance bacterial community-acquired infections Medicine R Arctic medicine. Tropical medicine RC955-962 Militza Guzmán Elsa Salazar Vicmaris Cordero Ana Castro Andreína Villanueva Hectorina Rodulfo Marcos De Donato Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
topic_facet |
Escherichia coli urologic diseases beta-lactamases drug resistance bacterial community-acquired infections Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: The treatment of urinary tract infections has become more challenging due to the increasing frequency of multidrug-resistant Escherichia coli in human populations. Objective: To characterize multidrug-resistant E. coli isolates causing community-acquired urinary tract infections in Cumaná, Venezuela, and associate possible risk factors for infection by extended-spectrum beta-lactamases (ESBL)-producing isolates. Materials and methods: We included all the patients with urinary tract infections attending the urology outpatient consultation and emergency unit in the Hospital de Cumaná, Estado Sucre, Venezuela, from January through June, 2014. blaTEM, blaSHV and blaCTX-M genes detection was carried out by PCR. Results: We found a high prevalence of multidrug-resistant E. coli (25.2%) with 20.4% of the isolates producing ESBL. The ESBL-producing isolates showed a high frequency (66.7%) of simultaneous resistance to trimethoprim-sulphamethoxazole, fluoroquinolones and aminoglycosides compared to non-producing isolates (2.4%). Of the resistant isolates, 65.4% carried the blaTEM gene, 34.6% the blaCTX-M and 23.1% the blaSHV. The blaCTX-M genes detected belonged to the CTX-M-1 and CTX-M-2 groups. Plasmid transfer was demonstrated by in vitro conjugation in 17 of the 26 ESBL-producing isolates. All three genes detected were transferred to the transconjugants. Age over 60 years, complicated urinary tract infections and previous use of a catheter predisposed patients to infection by ESBL-producing E. coli. Conclusions: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. |
format |
Article in Journal/Newspaper |
author |
Militza Guzmán Elsa Salazar Vicmaris Cordero Ana Castro Andreína Villanueva Hectorina Rodulfo Marcos De Donato |
author_facet |
Militza Guzmán Elsa Salazar Vicmaris Cordero Ana Castro Andreína Villanueva Hectorina Rodulfo Marcos De Donato |
author_sort |
Militza Guzmán |
title |
Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
title_short |
Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
title_full |
Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
title_fullStr |
Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
title_full_unstemmed |
Multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by Escherichia coli in Venezuela |
title_sort |
multidrug resistance and risk factors associated with community-acquired urinary tract infections caused by escherichia coli in venezuela |
publisher |
Instituto Nacional de Salud |
publishDate |
2019 |
url |
https://doi.org/10.7705/biomedica.v39i2.4030 https://doaj.org/article/375ff93a78a340eba1bacf2a80d21407 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 39, Pp 96-107 (2019) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/4030 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v39i2.4030 https://doaj.org/article/375ff93a78a340eba1bacf2a80d21407 |
op_doi |
https://doi.org/10.7705/biomedica.v39i2.4030 |
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Biomédica |
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39 |
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96 |
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107 |
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